DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations
The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated fr...
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| Veröffentlicht in: | European journal of human genetics : EJHG 2001-10, Vol.9 (10), p.765-772 |
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| creator | Dard, P Lefranc, M P Osipova, L Sanchez-Mazas, A |
| description | The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m(5,10,11,13,14) haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas. |
| doi_str_mv | 10.1038/sj.ejhg.5200700 |
| format | Article |
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By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m(5,10,11,13,14) haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas.</description><identifier>ISSN: 1018-4813</identifier><identifier>EISSN: 1476-5438</identifier><identifier>DOI: 10.1038/sj.ejhg.5200700</identifier><identifier>PMID: 11781688</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Alleles ; Allotypes ; Asia, Western ; Base Sequence ; Cloning ; Deoxyribonucleic acid ; DNA ; Ethnic Groups - genetics ; Europe ; Female ; Gene Frequency ; Gene polymorphism ; Genes ; Genetics ; Haplotypes ; Haplotypes - genetics ; Humans ; Immunoglobulin gamma-Chains - genetics ; Immunoglobulins ; Male ; Molecular evolution ; Multiple myeloma ; Nucleotide sequence ; Phylogeny ; Polymorphism, Genetic - genetics ; Polymorphism, Restriction Fragment Length ; Population genetics ; Population studies ; Senegal ; Serology ; Siberia ; Studies</subject><ispartof>European journal of human genetics : EJHG, 2001-10, Vol.9 (10), p.765-772</ispartof><rights>Copyright Nature Publishing Group Oct 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-be9d3fdd18fae4299f73bdf8a0a514a7b31440e7a6fe068870ec9de496b7fa53</citedby><cites>FETCH-LOGICAL-c393t-be9d3fdd18fae4299f73bdf8a0a514a7b31440e7a6fe068870ec9de496b7fa53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11781688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dard, P</creatorcontrib><creatorcontrib>Lefranc, M P</creatorcontrib><creatorcontrib>Osipova, L</creatorcontrib><creatorcontrib>Sanchez-Mazas, A</creatorcontrib><title>DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations</title><title>European journal of human genetics : EJHG</title><addtitle>Eur J Hum Genet</addtitle><description>The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m(5,10,11,13,14) haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas.</description><subject>Alleles</subject><subject>Allotypes</subject><subject>Asia, Western</subject><subject>Base Sequence</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ethnic Groups - genetics</subject><subject>Europe</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Gene polymorphism</subject><subject>Genes</subject><subject>Genetics</subject><subject>Haplotypes</subject><subject>Haplotypes - genetics</subject><subject>Humans</subject><subject>Immunoglobulin gamma-Chains - genetics</subject><subject>Immunoglobulins</subject><subject>Male</subject><subject>Molecular evolution</subject><subject>Multiple myeloma</subject><subject>Nucleotide sequence</subject><subject>Phylogeny</subject><subject>Polymorphism, Genetic - genetics</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population genetics</subject><subject>Population studies</subject><subject>Senegal</subject><subject>Serology</subject><subject>Siberia</subject><subject>Studies</subject><issn>1018-4813</issn><issn>1476-5438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1PwzAMhiME4vvMDUUc4NSRLGmTHtGAMWmCC_fIbR3WKm1K0yLt35OJSUgcONmyH1t-_RJyxdmMM6HvQzPDZvMxS-eMKcYOyCmXKktSKfRhzBnXidRcnJCzEBrGYlPxY3LCudI80_qUVI-vDzTg54RdifQLhhqK2tXjlnpLV8uXpaDgHDoMFELwZQ0jVnT0dNwgbaHu6FK0dAO98-O2j1SsbKYWOtr7fnIw1r4LF-TIggt4uY_n5P356X3xkqzflqvFwzopRS7GpMC8EraquLaAcp7nVomishoYpFyCKgSXkqGCzCKL1yuGZV6hzLNCWUjFObn7WdsPPgoKo2nrUKJz0KGfglGp1HkqFYvk7f_kXOSpljvw5g_Y-Gnooggzjz8UnOksQvc_UDn4EAa0ph_qFoat4czsbDKhMTubzN6mOHG9XzsVLVa__N4X8Q2c-o8M</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Dard, P</creator><creator>Lefranc, M P</creator><creator>Osipova, L</creator><creator>Sanchez-Mazas, A</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20011001</creationdate><title>DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations</title><author>Dard, P ; Lefranc, M P ; Osipova, L ; Sanchez-Mazas, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-be9d3fdd18fae4299f73bdf8a0a514a7b31440e7a6fe068870ec9de496b7fa53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alleles</topic><topic>Allotypes</topic><topic>Asia, Western</topic><topic>Base Sequence</topic><topic>Cloning</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ethnic Groups - genetics</topic><topic>Europe</topic><topic>Female</topic><topic>Gene Frequency</topic><topic>Gene polymorphism</topic><topic>Genes</topic><topic>Genetics</topic><topic>Haplotypes</topic><topic>Haplotypes - genetics</topic><topic>Humans</topic><topic>Immunoglobulin gamma-Chains - genetics</topic><topic>Immunoglobulins</topic><topic>Male</topic><topic>Molecular evolution</topic><topic>Multiple myeloma</topic><topic>Nucleotide sequence</topic><topic>Phylogeny</topic><topic>Polymorphism, Genetic - genetics</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Senegal</topic><topic>Serology</topic><topic>Siberia</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dard, P</creatorcontrib><creatorcontrib>Lefranc, M P</creatorcontrib><creatorcontrib>Osipova, L</creatorcontrib><creatorcontrib>Sanchez-Mazas, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>European journal of human genetics : EJHG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dard, P</au><au>Lefranc, M P</au><au>Osipova, L</au><au>Sanchez-Mazas, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations</atitle><jtitle>European journal of human genetics : EJHG</jtitle><addtitle>Eur J Hum Genet</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>9</volume><issue>10</issue><spage>765</spage><epage>772</epage><pages>765-772</pages><issn>1018-4813</issn><eissn>1476-5438</eissn><abstract>The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m(5,10,11,13,14) haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>11781688</pmid><doi>10.1038/sj.ejhg.5200700</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alleles Allotypes Asia, Western Base Sequence Cloning Deoxyribonucleic acid DNA Ethnic Groups - genetics Europe Female Gene Frequency Gene polymorphism Genes Genetics Haplotypes Haplotypes - genetics Humans Immunoglobulin gamma-Chains - genetics Immunoglobulins Male Molecular evolution Multiple myeloma Nucleotide sequence Phylogeny Polymorphism, Genetic - genetics Polymorphism, Restriction Fragment Length Population genetics Population studies Senegal Serology Siberia Studies |
| title | DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations |
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