Microbial Biosynthesis of Alkanes
Alkanes, the major constituents of gasoline, diesel, and jet fuel, are naturally produced by diverse species; however, the genetics and biochemistry behind this biology have remained elusive. Here we describe the discovery of an alkane biosynthesis pathway from cyanobacteria. The pathway consists of...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2010-07, Vol.329 (5991), p.559-562 |
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| creator | Schirmer, Andreas Rude, Mathew A Li, Xuezhi Popova, Emanuela del Cardayre, Stephen B |
| description | Alkanes, the major constituents of gasoline, diesel, and jet fuel, are naturally produced by diverse species; however, the genetics and biochemistry behind this biology have remained elusive. Here we describe the discovery of an alkane biosynthesis pathway from cyanobacteria. The pathway consists of an acyl-acyl carrier protein reductase and an aldehyde decarbonylase, which together convert intermediates of fatty acid metabolism to alkanes and alkenes. The aldehyde decarbonylase is related to the broadly functional nonheme diiron enzymes. Heterologous expression of the alkane operon in Escherichia coli leads to the production and secretion of C13 to C17 mixtures of alkanes and alkenes. These genes and enzymes can now be leveraged for the simple and direct conversion of renewable raw materials to fungible hydrocarbon fuels. |
| doi_str_mv | 10.1126/science.1187936 |
| format | Article |
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Here we describe the discovery of an alkane biosynthesis pathway from cyanobacteria. The pathway consists of an acyl-acyl carrier protein reductase and an aldehyde decarbonylase, which together convert intermediates of fatty acid metabolism to alkanes and alkenes. The aldehyde decarbonylase is related to the broadly functional nonheme diiron enzymes. Heterologous expression of the alkane operon in Escherichia coli leads to the production and secretion of C13 to C17 mixtures of alkanes and alkenes. These genes and enzymes can now be leveraged for the simple and direct conversion of renewable raw materials to fungible hydrocarbon fuels.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1187936</identifier><identifier>PMID: 20671186</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Acyl Carrier Protein - metabolism ; Aldehyde-Lyases - chemistry ; Aldehyde-Lyases - genetics ; Aldehyde-Lyases - metabolism ; Aldehydes ; Aldehydes - metabolism ; Alkanes ; Alkanes - metabolism ; Alkenes ; Alkenes - metabolism ; Bacteria ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biochemistry ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biosynthesis ; Biosynthetic Pathways ; Biotechnology ; Chemical compounds ; Cyanobacteria ; Cyanobacteria - enzymology ; Cyanobacteria - genetics ; Cyanobacteria - metabolism ; Enzymes ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Fatty Acids - metabolism ; Fatty alcohols ; Fatty Alcohols - metabolism ; Ferredoxins ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Hydrocarbons ; Microbiology ; Mission oriented research ; Operon ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Physiology and metabolism ; Protein Conformation ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Ribonucleotides ; Substrate Specificity ; Synechococcus - enzymology ; Synechococcus - genetics ; Synechococcus - metabolism</subject><ispartof>Science (American Association for the Advancement of Science), 2010-07, Vol.329 (5991), p.559-562</ispartof><rights>2010 American Association for the Advancement of Science</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010, American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-e8ccd1fd6f792e230041bd5e8c916205769615b1e6f9f6c4df80ce60d075b21b3</citedby><cites>FETCH-LOGICAL-c497t-e8ccd1fd6f792e230041bd5e8c916205769615b1e6f9f6c4df80ce60d075b21b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40799644$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40799644$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23083699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20671186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schirmer, Andreas</creatorcontrib><creatorcontrib>Rude, Mathew A</creatorcontrib><creatorcontrib>Li, Xuezhi</creatorcontrib><creatorcontrib>Popova, Emanuela</creatorcontrib><creatorcontrib>del Cardayre, Stephen B</creatorcontrib><title>Microbial Biosynthesis of Alkanes</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Alkanes, the major constituents of gasoline, diesel, and jet fuel, are naturally produced by diverse species; however, the genetics and biochemistry behind this biology have remained elusive. 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These genes and enzymes can now be leveraged for the simple and direct conversion of renewable raw materials to fungible hydrocarbon fuels.</description><subject>Acyl Carrier Protein - metabolism</subject><subject>Aldehyde-Lyases - chemistry</subject><subject>Aldehyde-Lyases - genetics</subject><subject>Aldehyde-Lyases - metabolism</subject><subject>Aldehydes</subject><subject>Aldehydes - metabolism</subject><subject>Alkanes</subject><subject>Alkanes - metabolism</subject><subject>Alkenes</subject><subject>Alkenes - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways</subject><subject>Biotechnology</subject><subject>Chemical compounds</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - enzymology</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - metabolism</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Fatty Acids - metabolism</subject><subject>Fatty alcohols</subject><subject>Fatty Alcohols - metabolism</subject><subject>Ferredoxins</subject><subject>Fundamental and applied biological sciences. 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Here we describe the discovery of an alkane biosynthesis pathway from cyanobacteria. The pathway consists of an acyl-acyl carrier protein reductase and an aldehyde decarbonylase, which together convert intermediates of fatty acid metabolism to alkanes and alkenes. The aldehyde decarbonylase is related to the broadly functional nonheme diiron enzymes. Heterologous expression of the alkane operon in Escherichia coli leads to the production and secretion of C13 to C17 mixtures of alkanes and alkenes. These genes and enzymes can now be leveraged for the simple and direct conversion of renewable raw materials to fungible hydrocarbon fuels.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>20671186</pmid><doi>10.1126/science.1187936</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2010-07, Vol.329 (5991), p.559-562 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Acyl Carrier Protein - metabolism Aldehyde-Lyases - chemistry Aldehyde-Lyases - genetics Aldehyde-Lyases - metabolism Aldehydes Aldehydes - metabolism Alkanes Alkanes - metabolism Alkenes Alkenes - metabolism Bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biochemistry Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biosynthesis Biosynthetic Pathways Biotechnology Chemical compounds Cyanobacteria Cyanobacteria - enzymology Cyanobacteria - genetics Cyanobacteria - metabolism Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fatty Acids - metabolism Fatty alcohols Fatty Alcohols - metabolism Ferredoxins Fundamental and applied biological sciences. Psychology Genes, Bacterial Hydrocarbons Microbiology Mission oriented research Operon Oxidoreductases - genetics Oxidoreductases - metabolism Physiology and metabolism Protein Conformation Recombinant Proteins - genetics Recombinant Proteins - metabolism Ribonucleotides Substrate Specificity Synechococcus - enzymology Synechococcus - genetics Synechococcus - metabolism |
| title | Microbial Biosynthesis of Alkanes |
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