Cell labeling with the positive MR contrast agent Gadofluorine M

The purpose of this study was to label human monocytes with Gadofluorine M by simple incubation for subsequent cell depiction at 1.5 and 3 T. Gadofluorine M displays a high r(1) relaxivity and is spontaneously phagocytosed by macrophages. Human monocytes were incubated with Gadofluorine M-Cy at vary...

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Veröffentlicht in:	European radiology 2007-05, Vol.17 (5), p.1226-1234
Hauptverfasser:	Henning, Tobias D, Saborowski, Olaf, Golovko, Daniel, Boddington, Sophie, Bauer, Jan S, Fu, Yanjun, Meier, Reinhard, Pietsch, Hubertus, Sennino, Barbara, McDonald, Donald M, Daldrup-Link, Heike E
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell therapy Cell viability Cells, Cultured Contrast agents Contrast media Contrast Media - pharmacokinetics Fluorescence Fluorescence microscopy Gadolinium Humans In vivo methods and tests Internalization Labeling Labels Leukocytes Macrophages Magnetic resonance imaging Magnetic Resonance Imaging - methods Microscopy Microscopy, Confocal Monocytes Monocytes - metabolism Organometallic Compounds - pharmacokinetics Spectrophotometry, Atomic Staining and Labeling Stem cells
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