Lucigenin-derived chemiluminescence in intact isolated mitochondria

There are many data both in favor and against the use of lucigenin as a probe for superoxide anion (SA) in mitochondria, cells, and simple enzymatic systems. In the present work high concentrations (50-400 micro M) of lucigenin were used for continuous recording of rapid and reversible changes in th...

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Veröffentlicht in:	Biochemistry (Moscow) 2002-11, Vol.67 (11), p.1262-1270
Hauptverfasser:	Kruglov, A G, Yurkov, I S, Teplova, V V, Evtodienko, Yu V
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines - chemistry Adenosine diphosphate Adenosine Diphosphate - metabolism Adenosine Diphosphate - pharmacology Adenosine Triphosphate - chemistry Adenosine Triphosphate - metabolism Animals Calcium - chemistry Calcium - metabolism Calcium - pharmacology Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology Chemiluminescence Electric potential Electron Transport Electron Transport Complex III - antagonists & inhibitors Electron Transport Complex III - metabolism Enzyme Inhibitors - pharmacology Intracellular Membranes - drug effects Intracellular Membranes - metabolism Intracellular Membranes - physiology Luminescent Measurements Magnesium - chemistry Magnesium - metabolism Membrane Potentials - drug effects Membrane Potentials - physiology Mitochondria Mitochondria, Liver - metabolism Oxidation Pyruvic Acid - metabolism Rats Rats, Wistar Succinic Acid - metabolism Superoxides - metabolism
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