Development of antibody to human GM3 synthase and immunodetection of the enzyme in human tissues

Polyclonal antibody was raised to a cloned fragment of human GM3 synthase. Affinity purified R27C1 antibody to the tagged recombinant protein inhibited GM3 synthase activity in human liver and HL-60 cells in a dose-dependent manner. However, the R27C1 antibody did not affect liver sialyltransferase...

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Veröffentlicht in:	Biochemistry (Moscow) 2004-03, Vol.69 (3), p.275-280
Hauptverfasser:	Golovanova, N K, Samovilova, N N, Gracheva, E V, Peklo, M M, Vlasik, T N, Sobolev, A Yu, Jurchenko, Yu V, Prokazova, N V
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Fetoproteins - chemistry Animals Antibodies Antibodies - immunology Antibodies - pharmacology Aorta - enzymology Aorta - immunology Aorta - pathology Arteriosclerosis - enzymology Arteriosclerosis - immunology Arteriosclerosis - pathology Asialoglycoproteins - chemistry Brain - enzymology Brain - immunology Fetuins HL-60 Cells Humans Kinetics Liver Liver - enzymology Liver - immunology Molecular Weight Organ Specificity - immunology Proteins Rabbits Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism Rodents Sialyltransferases - antagonists & inhibitors Sialyltransferases - chemistry Sialyltransferases - genetics Sialyltransferases - immunology Sialyltransferases - metabolism Tunica Intima - enzymology Tunica Intima - immunology Tunica Intima - pathology Viral antibodies
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