Affinity labeling of flap-endonuclease FEN-1 by photoreactive DNAs

Eukaryotic flap-endonuclease (FEN-1) is 42-kD single-subunit structure-specific nuclease that cleaves 5'-flap strands of the branched DNA structure and possesses 5'-exonuclease activity. FEN-1 participates in DNA replication, repair, and recombination. The interaction of FEN-1 with DNA str...

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Veröffentlicht in:	Biochemistry (Moscow) 2001-07, Vol.66 (7), p.733-739
Hauptverfasser:	Khlimankov, D Y, Rechkunova, N I, Kolpashchikov, D M, Khodyreva, S N, Lavrik, O I
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Deoxyribonucleic acid DNA DNA Polymerase beta - metabolism DNA Primers - genetics DNA Primers - metabolism DNA Repair DNA Replication Endodeoxyribonucleases - chemistry Endodeoxyribonucleases - metabolism Fibroblasts - metabolism Flap Endonucleases Labeling Mice Oligonucleotides - chemistry Oligonucleotides - metabolism Photoaffinity Labels - chemistry Photoaffinity Labels - metabolism Substrate Specificity
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