Isolation, purification, and characterization of catalase from the methylotrophic yeast Pichia pastoris

Catalase (CATpp) with molecular weight 223 kD was isolated from the methylotrophic yeast Pichia pastoris and purified 90-fold by ion-exchange chromatography and gel filtration. Quantitative parameters of absorption and CD spectra of CATpp solutions and of its membrane-concentrated form (CATpp-conc)...

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Veröffentlicht in:	Biochemistry (Moscow) 2001-06, Vol.66 (6), p.646-657
Hauptverfasser:	Potapovich, M V, Eryomin, A N, Artzukevich, I M, Chernikevich, I P, Metelitza, D I
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Buffers Candida boidinii Catalase - chemistry Catalase - isolation & purification Catalase - metabolism Catalysis Cattle Cavitation Chromatography Circular Dichroism Enzyme Stability Enzymes Hydrogen Peroxide - metabolism Hydrogen-Ion Concentration Inactivation Kinetics Molecular Weight Peroxisomes - chemistry Pichia - enzymology Pichia pastoris Temperature Yeasts
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