On-line control of cellular adhesion with impedance measurements using interdigitated electrode structures
Critical parameters to be assessed in cell culture are the number of viable cells and cell viability. Growth, product formation, toxicity effects and the overall success of cell culture can depend largely on these. With interdigitated electrode structures (IDES) adherent cells are cultured directly...
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| Veröffentlicht in: | Medical & biological engineering & computing 1998-05, Vol.36 (3), p.365-370 |
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| creator | EHRET, R BAUMANN, W BRISCHWEIN, M SCHWINDE, A WOLF, B |
| description | Critical parameters to be assessed in cell culture are the number of viable cells and cell viability. Growth, product formation, toxicity effects and the overall success of cell culture can depend largely on these. With interdigitated electrode structures (IDES) adherent cells are cultured directly on a pair of interdigitated electrodes, and the impedance of the system gives insight into the adhesive behaviour of the cells. The signal is influenced by the changes in number, growth and morphological behaviour of adherently growing cells, mainly owing to the insulating effects of the cell membranes. Five different cell lines are used, and their divergent behaviour is monitored over a period of four days, from inoculation of the cells to killing of the cells at the end of the experiments. Even when the cells from close monolayers, great fluctuations in the impedance signal can be observed. Nevertheless, for a more complete description of cellular systems, other parameters, such as acidification and respiration, have to be included in the measuring system. |
| doi_str_mv | 10.1007/BF02522485 |
| format | Article |
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Growth, product formation, toxicity effects and the overall success of cell culture can depend largely on these. With interdigitated electrode structures (IDES) adherent cells are cultured directly on a pair of interdigitated electrodes, and the impedance of the system gives insight into the adhesive behaviour of the cells. The signal is influenced by the changes in number, growth and morphological behaviour of adherently growing cells, mainly owing to the insulating effects of the cell membranes. Five different cell lines are used, and their divergent behaviour is monitored over a period of four days, from inoculation of the cells to killing of the cells at the end of the experiments. Even when the cells from close monolayers, great fluctuations in the impedance signal can be observed. 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Growth, product formation, toxicity effects and the overall success of cell culture can depend largely on these. With interdigitated electrode structures (IDES) adherent cells are cultured directly on a pair of interdigitated electrodes, and the impedance of the system gives insight into the adhesive behaviour of the cells. The signal is influenced by the changes in number, growth and morphological behaviour of adherently growing cells, mainly owing to the insulating effects of the cell membranes. Five different cell lines are used, and their divergent behaviour is monitored over a period of four days, from inoculation of the cells to killing of the cells at the end of the experiments. Even when the cells from close monolayers, great fluctuations in the impedance signal can be observed. Nevertheless, for a more complete description of cellular systems, other parameters, such as acidification and respiration, have to be included in the measuring system.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cell Adhesion</subject><subject>Cell culture</subject><subject>Cell Physiological Phenomena</subject><subject>Cells, Cultured</subject><subject>Electric Impedance</subject><subject>Electrodes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Methods. Procedures. 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| ispartof | Medical & biological engineering & computing, 1998-05, Vol.36 (3), p.365-370 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Cell Adhesion Cell culture Cell Physiological Phenomena Cells, Cultured Electric Impedance Electrodes Fundamental and applied biological sciences. Psychology Humans Methods. Procedures. Technologies Microscopy, Electron, Scanning Tumor Cells, Cultured Various methods and equipments |
| title | On-line control of cellular adhesion with impedance measurements using interdigitated electrode structures |
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