DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study
Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH‐dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues...
Gespeichert in:
| Veröffentlicht in: | Journal of Raman spectroscopy 2005-11, Vol.36 (11), p.1047-1051 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1051 |
|---|---|
| container_issue | 11 |
| container_start_page | 1047 |
| container_title | Journal of Raman spectroscopy |
| container_volume | 36 |
| creator | Muntean, Cristina M. Dostál, Lubomir Misselwitz, Rolf Welfle, Heinz |
| description | Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH‐dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues upon lowering the pH to 3.45. Adenine protonation is connected with the disruption of AT base pairs. Protonation of GC base pairs is indicated at somewhat lower pH than that of AT base pairs, namely at pH 3.8, and continues upon lowering the pH to 3.45. At pH 3.8 unstacking of thymine residues is indicated, and spectral markers for the unstacking of adenine and cytosine were found at pH 3.45. Changes of the DNA backbone are indicated by spectral changes of conformational marker bands at 898 and 1423 cm−1. Copyright © 2005 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jrs.1404 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754879255</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>754879255</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2654-c7516cb6cc7666cdf0cfeadda43d426cd95eeca69fdde233730a8139998479853</originalsourceid><addsrcrecordid>eNp10F9LwzAUBfAgCs4p-BHypqCdafOv8W2buqlzwlT2GGJ6i51dW5PWuW9vx0TwwacLlx-Hw0HoOCS9kJDoYuF8L2SE7aBOSJQMGOd8F3UIlTIgLBb76MD7BSFEKRF20P3VtI997RpbNw6wqXFernA1xp8mb8Cf46zA9RvgyoGHwgIuU_xQRGc4Kwt_iQ2emaUp2oQmWR-ivdTkHo5-bhe93Fw_D8fB5HF0O-xPAhsJzgIreSjsq7BWCiFskhKbgkkSw2jCovahOIA1QqVJAhGlkhITh1QpFTOpYk676GSbW7nyoy1Z62XmLeS5KaBsvJacxVJFfCNPt9K60nsHqa5ctjRurUOiN3Ppdi69maulwZaushzW_zp9N3v66zNfw9evN-5dC0kl1_PpSA9iPiB0PNcD-g3n9HkP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>754879255</pqid></control><display><type>article</type><title>DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Muntean, Cristina M. ; Dostál, Lubomir ; Misselwitz, Rolf ; Welfle, Heinz</creator><creatorcontrib>Muntean, Cristina M. ; Dostál, Lubomir ; Misselwitz, Rolf ; Welfle, Heinz</creatorcontrib><description>Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH‐dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues upon lowering the pH to 3.45. Adenine protonation is connected with the disruption of AT base pairs. Protonation of GC base pairs is indicated at somewhat lower pH than that of AT base pairs, namely at pH 3.8, and continues upon lowering the pH to 3.45. At pH 3.8 unstacking of thymine residues is indicated, and spectral markers for the unstacking of adenine and cytosine were found at pH 3.45. Changes of the DNA backbone are indicated by spectral changes of conformational marker bands at 898 and 1423 cm−1. Copyright © 2005 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0377-0486</identifier><identifier>EISSN: 1097-4555</identifier><identifier>DOI: 10.1002/jrs.1404</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>calf thymus DNA ; difference Raman spectroscopy ; difference spectra ; DNA protonation</subject><ispartof>Journal of Raman spectroscopy, 2005-11, Vol.36 (11), p.1047-1051</ispartof><rights>Copyright © 2005 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2654-c7516cb6cc7666cdf0cfeadda43d426cd95eeca69fdde233730a8139998479853</citedby><cites>FETCH-LOGICAL-c2654-c7516cb6cc7666cdf0cfeadda43d426cd95eeca69fdde233730a8139998479853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjrs.1404$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjrs.1404$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids></links><search><creatorcontrib>Muntean, Cristina M.</creatorcontrib><creatorcontrib>Dostál, Lubomir</creatorcontrib><creatorcontrib>Misselwitz, Rolf</creatorcontrib><creatorcontrib>Welfle, Heinz</creatorcontrib><title>DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study</title><title>Journal of Raman spectroscopy</title><addtitle>J. Raman Spectrosc</addtitle><description>Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH‐dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues upon lowering the pH to 3.45. Adenine protonation is connected with the disruption of AT base pairs. Protonation of GC base pairs is indicated at somewhat lower pH than that of AT base pairs, namely at pH 3.8, and continues upon lowering the pH to 3.45. At pH 3.8 unstacking of thymine residues is indicated, and spectral markers for the unstacking of adenine and cytosine were found at pH 3.45. Changes of the DNA backbone are indicated by spectral changes of conformational marker bands at 898 and 1423 cm−1. Copyright © 2005 John Wiley & Sons, Ltd.</description><subject>calf thymus DNA</subject><subject>difference Raman spectroscopy</subject><subject>difference spectra</subject><subject>DNA protonation</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp10F9LwzAUBfAgCs4p-BHypqCdafOv8W2buqlzwlT2GGJ6i51dW5PWuW9vx0TwwacLlx-Hw0HoOCS9kJDoYuF8L2SE7aBOSJQMGOd8F3UIlTIgLBb76MD7BSFEKRF20P3VtI997RpbNw6wqXFernA1xp8mb8Cf46zA9RvgyoGHwgIuU_xQRGc4Kwt_iQ2emaUp2oQmWR-ivdTkHo5-bhe93Fw_D8fB5HF0O-xPAhsJzgIreSjsq7BWCiFskhKbgkkSw2jCovahOIA1QqVJAhGlkhITh1QpFTOpYk676GSbW7nyoy1Z62XmLeS5KaBsvJacxVJFfCNPt9K60nsHqa5ctjRurUOiN3Ppdi69maulwZaushzW_zp9N3v66zNfw9evN-5dC0kl1_PpSA9iPiB0PNcD-g3n9HkP</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Muntean, Cristina M.</creator><creator>Dostál, Lubomir</creator><creator>Misselwitz, Rolf</creator><creator>Welfle, Heinz</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>200511</creationdate><title>DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study</title><author>Muntean, Cristina M. ; Dostál, Lubomir ; Misselwitz, Rolf ; Welfle, Heinz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2654-c7516cb6cc7666cdf0cfeadda43d426cd95eeca69fdde233730a8139998479853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>calf thymus DNA</topic><topic>difference Raman spectroscopy</topic><topic>difference spectra</topic><topic>DNA protonation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muntean, Cristina M.</creatorcontrib><creatorcontrib>Dostál, Lubomir</creatorcontrib><creatorcontrib>Misselwitz, Rolf</creatorcontrib><creatorcontrib>Welfle, Heinz</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of Raman spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muntean, Cristina M.</au><au>Dostál, Lubomir</au><au>Misselwitz, Rolf</au><au>Welfle, Heinz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study</atitle><jtitle>Journal of Raman spectroscopy</jtitle><addtitle>J. Raman Spectrosc</addtitle><date>2005-11</date><risdate>2005</risdate><volume>36</volume><issue>11</issue><spage>1047</spage><epage>1051</epage><pages>1047-1051</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><abstract>Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH‐dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues upon lowering the pH to 3.45. Adenine protonation is connected with the disruption of AT base pairs. Protonation of GC base pairs is indicated at somewhat lower pH than that of AT base pairs, namely at pH 3.8, and continues upon lowering the pH to 3.45. At pH 3.8 unstacking of thymine residues is indicated, and spectral markers for the unstacking of adenine and cytosine were found at pH 3.45. Changes of the DNA backbone are indicated by spectral changes of conformational marker bands at 898 and 1423 cm−1. Copyright © 2005 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jrs.1404</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0377-0486 |
| ispartof | Journal of Raman spectroscopy, 2005-11, Vol.36 (11), p.1047-1051 |
| issn | 0377-0486 1097-4555 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_754879255 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | calf thymus DNA difference Raman spectroscopy difference spectra DNA protonation |
| title | DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T11%3A39%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20structure%20at%20low%20pH%20values,%20in%20the%20presence%20of%20Mn2+%20ions:%20a%20Raman%20study&rft.jtitle=Journal%20of%20Raman%20spectroscopy&rft.au=Muntean,%20Cristina%20M.&rft.date=2005-11&rft.volume=36&rft.issue=11&rft.spage=1047&rft.epage=1051&rft.pages=1047-1051&rft.issn=0377-0486&rft.eissn=1097-4555&rft_id=info:doi/10.1002/jrs.1404&rft_dat=%3Cproquest_cross%3E754879255%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=754879255&rft_id=info:pmid/&rfr_iscdi=true |