Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice
Please cite this paper as: Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Experimental Dermatology 2010; 19: e37–e43. : The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively i...
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| creator | Choi, Sun Eun Jeong, Mi Sook Kang, Myung Joo Lee, Do Ik Joo, Seong Soo Lee, Chung Soo Bang, Hyoweon Lee, Mi-Kyung Myung, Soon-Chul Choi, Young Wook Lee, Kap-sok Seo, Seong Jun Lee, Min Won |
| description | Please cite this paper as: Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Experimental Dermatology 2010; 19: e37–e43.
: The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively in Korea, has been known to exert antioxidative, anti‐inflammatory, anti‐cancer and immune response inhibitory effects. The antioxidative effect of ORE was observed on the superoxide and 1,1‐diphenyl‐2‐picrylhydrazyl radical, as well as on the expression of inducible nitric oxide synthase and cyclooxygenase‐2 in lipopolysaccharide‐treated RAW264.7 macrophages. The statistically significant inhibitory action of ORE against production of cytokines induced by bacterial products or by interleukin (IL)‐1β, free radicals and nitrogen species, and a corresponding increase in cellular calcium concentration because of ORE were confirmed in bone marrow and spleen dendritic cells that are known to play important functions in the development and advancement of atopic dermatitis (AD). It was thus expected that ORE would exert a beneficial effect in the treatment of AD. A study on the pharmaceutical benefits of ORE against AD has not yet been conducted in vivo. We therefore used an in vivo AD animal model, namely the NC/Nga mice, and by applying ORE onto the animals through skin application as well as intraperitoneal injection, we attempted to evaluate the benefits of ORE in this system. Evaluation of ORE was conducted by following the SCORE method to score the effect, as well as by measuring the Th2 cytokines IL‐4, IL‐5 and IL‐13 levels from serum and lymphocytes, and IgE and eosinophil levels from serum. Additionally, the expression of mRNA and protein levels was estimated using real‐time polymerase chain reaction and Western blotting analysis. The following categories of clinical evaluation, Th2 cytokines IL‐4, IL‐5 and IL‐13 values, serum IgE levels, serum eosinophil levels, and mRNA and protein expression levels of iNOS and COX‐2, were evaluated from topical application and intraperitoneal injection groups of ORE. The effects of ORE on AD in NC/Nga mice were confirmed as being similar to the positive control group, while a significant difference with the negative control group was observed. The results presented in this report suggest that ORE might be beneficial in the treatment of AD. |
| doi_str_mv | 10.1111/j.1600-0625.2009.00961.x |
| format | Article |
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: The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively in Korea, has been known to exert antioxidative, anti‐inflammatory, anti‐cancer and immune response inhibitory effects. The antioxidative effect of ORE was observed on the superoxide and 1,1‐diphenyl‐2‐picrylhydrazyl radical, as well as on the expression of inducible nitric oxide synthase and cyclooxygenase‐2 in lipopolysaccharide‐treated RAW264.7 macrophages. The statistically significant inhibitory action of ORE against production of cytokines induced by bacterial products or by interleukin (IL)‐1β, free radicals and nitrogen species, and a corresponding increase in cellular calcium concentration because of ORE were confirmed in bone marrow and spleen dendritic cells that are known to play important functions in the development and advancement of atopic dermatitis (AD). It was thus expected that ORE would exert a beneficial effect in the treatment of AD. A study on the pharmaceutical benefits of ORE against AD has not yet been conducted in vivo. We therefore used an in vivo AD animal model, namely the NC/Nga mice, and by applying ORE onto the animals through skin application as well as intraperitoneal injection, we attempted to evaluate the benefits of ORE in this system. Evaluation of ORE was conducted by following the SCORE method to score the effect, as well as by measuring the Th2 cytokines IL‐4, IL‐5 and IL‐13 levels from serum and lymphocytes, and IgE and eosinophil levels from serum. Additionally, the expression of mRNA and protein levels was estimated using real‐time polymerase chain reaction and Western blotting analysis. The following categories of clinical evaluation, Th2 cytokines IL‐4, IL‐5 and IL‐13 values, serum IgE levels, serum eosinophil levels, and mRNA and protein expression levels of iNOS and COX‐2, were evaluated from topical application and intraperitoneal injection groups of ORE. The effects of ORE on AD in NC/Nga mice were confirmed as being similar to the positive control group, while a significant difference with the negative control group was observed. The results presented in this report suggest that ORE might be beneficial in the treatment of AD.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/j.1600-0625.2009.00961.x</identifier><identifier>PMID: 19849716</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Administration, Topical ; Alnus ; Alnus japonica ; Animal models ; Animals ; Atopic dermatitis ; Bark ; Bone marrow ; Calcium ; Cyclooxygenase 2 - metabolism ; Cyclooxygenase-2 ; Cytokines - metabolism ; Dendritic cells ; Dermatitis, Atopic - drug therapy ; Dermatitis, Atopic - metabolism ; Dermatology ; diarylheptanoid ; Diarylheptanoids - administration & dosage ; Diarylheptanoids - therapeutic use ; Disease Models, Animal ; Female ; Free radicals ; Gene expression ; Helper cells ; Immune response ; Immunoglobulin E ; Immunoglobulin E - blood ; Inflammation ; Injections, Intraperitoneal ; Interleukin 13 ; Interleukin 4 ; Interleukin 5 ; Leukocytes (eosinophilic) ; Lymphocytes T ; Macrophages ; Mice ; Mice, Inbred Strains ; mRNA ; NC/Nga mice ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Nitrogen ; oregonin ; Pharmaceuticals ; Plant Extracts - administration & dosage ; Plant Extracts - therapeutic use ; Polymerase chain reaction ; Reactive Oxygen Species - metabolism ; Skin ; Spleen ; Statistical analysis ; Topical application ; Treatment Outcome ; Western blotting</subject><ispartof>Experimental dermatology, 2010-08, Vol.19 (8), p.e37-e43</ispartof><rights>2009 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5531-3afd89b9b963722191c80d75a9f24a6e5549f410cd12533eaca70c393019650a3</citedby><cites>FETCH-LOGICAL-c5531-3afd89b9b963722191c80d75a9f24a6e5549f410cd12533eaca70c393019650a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0625.2009.00961.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0625.2009.00961.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19849716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Sun Eun</creatorcontrib><creatorcontrib>Jeong, Mi Sook</creatorcontrib><creatorcontrib>Kang, Myung Joo</creatorcontrib><creatorcontrib>Lee, Do Ik</creatorcontrib><creatorcontrib>Joo, Seong Soo</creatorcontrib><creatorcontrib>Lee, Chung Soo</creatorcontrib><creatorcontrib>Bang, Hyoweon</creatorcontrib><creatorcontrib>Lee, Mi-Kyung</creatorcontrib><creatorcontrib>Myung, Soon-Chul</creatorcontrib><creatorcontrib>Choi, Young Wook</creatorcontrib><creatorcontrib>Lee, Kap-sok</creatorcontrib><creatorcontrib>Seo, Seong Jun</creatorcontrib><creatorcontrib>Lee, Min Won</creatorcontrib><title>Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>Please cite this paper as: Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Experimental Dermatology 2010; 19: e37–e43.
: The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively in Korea, has been known to exert antioxidative, anti‐inflammatory, anti‐cancer and immune response inhibitory effects. The antioxidative effect of ORE was observed on the superoxide and 1,1‐diphenyl‐2‐picrylhydrazyl radical, as well as on the expression of inducible nitric oxide synthase and cyclooxygenase‐2 in lipopolysaccharide‐treated RAW264.7 macrophages. The statistically significant inhibitory action of ORE against production of cytokines induced by bacterial products or by interleukin (IL)‐1β, free radicals and nitrogen species, and a corresponding increase in cellular calcium concentration because of ORE were confirmed in bone marrow and spleen dendritic cells that are known to play important functions in the development and advancement of atopic dermatitis (AD). It was thus expected that ORE would exert a beneficial effect in the treatment of AD. A study on the pharmaceutical benefits of ORE against AD has not yet been conducted in vivo. We therefore used an in vivo AD animal model, namely the NC/Nga mice, and by applying ORE onto the animals through skin application as well as intraperitoneal injection, we attempted to evaluate the benefits of ORE in this system. Evaluation of ORE was conducted by following the SCORE method to score the effect, as well as by measuring the Th2 cytokines IL‐4, IL‐5 and IL‐13 levels from serum and lymphocytes, and IgE and eosinophil levels from serum. Additionally, the expression of mRNA and protein levels was estimated using real‐time polymerase chain reaction and Western blotting analysis. The following categories of clinical evaluation, Th2 cytokines IL‐4, IL‐5 and IL‐13 values, serum IgE levels, serum eosinophil levels, and mRNA and protein expression levels of iNOS and COX‐2, were evaluated from topical application and intraperitoneal injection groups of ORE. The effects of ORE on AD in NC/Nga mice were confirmed as being similar to the positive control group, while a significant difference with the negative control group was observed. The results presented in this report suggest that ORE might be beneficial in the treatment of AD.</description><subject>Administration, Topical</subject><subject>Alnus</subject><subject>Alnus japonica</subject><subject>Animal models</subject><subject>Animals</subject><subject>Atopic dermatitis</subject><subject>Bark</subject><subject>Bone marrow</subject><subject>Calcium</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cyclooxygenase-2</subject><subject>Cytokines - metabolism</subject><subject>Dendritic cells</subject><subject>Dermatitis, Atopic - drug therapy</subject><subject>Dermatitis, Atopic - metabolism</subject><subject>Dermatology</subject><subject>diarylheptanoid</subject><subject>Diarylheptanoids - administration & dosage</subject><subject>Diarylheptanoids - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Free radicals</subject><subject>Gene expression</subject><subject>Helper cells</subject><subject>Immune response</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin E - blood</subject><subject>Inflammation</subject><subject>Injections, Intraperitoneal</subject><subject>Interleukin 13</subject><subject>Interleukin 4</subject><subject>Interleukin 5</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>mRNA</subject><subject>NC/Nga mice</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Nitrogen</subject><subject>oregonin</subject><subject>Pharmaceuticals</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - therapeutic use</subject><subject>Polymerase chain reaction</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Skin</subject><subject>Spleen</subject><subject>Statistical analysis</subject><subject>Topical application</subject><subject>Treatment Outcome</subject><subject>Western blotting</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFu2yAUhlHVqc26vkLF3a7sHowBI_VmzbJmU5Zp0rb2DlGMK1LbuOBo6dsPN1F3uYEQRzrfd5D4EcIEcpLW5SYnHCADXrC8AJB5OpzkuyM0e20coxlI4BkXwE7R2xg3AERQwU7QKZFVKQXhM_S4aBprRuwbPPrBGd1iPQxtKkbne6z7Grt-DHqwwY2-t6nv-k0ypm6SfLAPvnepTvDLBFzb0CV7dDGheD2_XD9o3Dlj36E3jW6jPT_cZ-jnp8WP-TJbfbv5PP-wygxjlGRUN3Ul79PmVBQFkcRUUAumZVOUmlvGStmUBExNCkap1UYLMFRSIJIz0PQMvd_PHYJ_2to4qs5FY9tW99ZvoxKsrISQAP8maQkEoKwSWe1JE3yMwTZqCK7T4VkRUFMmaqOmr1fT16spE_WSidol9eLwyPa-s_Vf8RBCAq72wG_X2uf_HqwWdx9TkfRsr7s42t2rrsOj4lPe6nZ9o1Zfrn8tl19v1Xf6B-7zqSI</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Choi, Sun Eun</creator><creator>Jeong, Mi Sook</creator><creator>Kang, Myung Joo</creator><creator>Lee, Do Ik</creator><creator>Joo, Seong Soo</creator><creator>Lee, Chung Soo</creator><creator>Bang, Hyoweon</creator><creator>Lee, Mi-Kyung</creator><creator>Myung, Soon-Chul</creator><creator>Choi, Young Wook</creator><creator>Lee, Kap-sok</creator><creator>Seo, Seong Jun</creator><creator>Lee, Min Won</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>201008</creationdate><title>Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice</title><author>Choi, Sun Eun ; Jeong, Mi Sook ; Kang, Myung Joo ; Lee, Do Ik ; Joo, Seong Soo ; Lee, Chung Soo ; Bang, Hyoweon ; Lee, Mi-Kyung ; Myung, Soon-Chul ; Choi, Young Wook ; Lee, Kap-sok ; Seo, Seong Jun ; Lee, Min Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5531-3afd89b9b963722191c80d75a9f24a6e5549f410cd12533eaca70c393019650a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Administration, Topical</topic><topic>Alnus</topic><topic>Alnus japonica</topic><topic>Animal models</topic><topic>Animals</topic><topic>Atopic dermatitis</topic><topic>Bark</topic><topic>Bone marrow</topic><topic>Calcium</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Cyclooxygenase-2</topic><topic>Cytokines - metabolism</topic><topic>Dendritic cells</topic><topic>Dermatitis, Atopic - drug therapy</topic><topic>Dermatitis, Atopic - metabolism</topic><topic>Dermatology</topic><topic>diarylheptanoid</topic><topic>Diarylheptanoids - administration & dosage</topic><topic>Diarylheptanoids - therapeutic use</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Free radicals</topic><topic>Gene expression</topic><topic>Helper cells</topic><topic>Immune response</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin E - blood</topic><topic>Inflammation</topic><topic>Injections, Intraperitoneal</topic><topic>Interleukin 13</topic><topic>Interleukin 4</topic><topic>Interleukin 5</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>mRNA</topic><topic>NC/Nga mice</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>Nitric-oxide synthase</topic><topic>Nitrogen</topic><topic>oregonin</topic><topic>Pharmaceuticals</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - therapeutic use</topic><topic>Polymerase chain reaction</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Skin</topic><topic>Spleen</topic><topic>Statistical analysis</topic><topic>Topical application</topic><topic>Treatment Outcome</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Sun Eun</creatorcontrib><creatorcontrib>Jeong, Mi Sook</creatorcontrib><creatorcontrib>Kang, Myung Joo</creatorcontrib><creatorcontrib>Lee, Do Ik</creatorcontrib><creatorcontrib>Joo, Seong Soo</creatorcontrib><creatorcontrib>Lee, Chung Soo</creatorcontrib><creatorcontrib>Bang, Hyoweon</creatorcontrib><creatorcontrib>Lee, Mi-Kyung</creatorcontrib><creatorcontrib>Myung, Soon-Chul</creatorcontrib><creatorcontrib>Choi, Young Wook</creatorcontrib><creatorcontrib>Lee, Kap-sok</creatorcontrib><creatorcontrib>Seo, Seong Jun</creatorcontrib><creatorcontrib>Lee, Min Won</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Sun Eun</au><au>Jeong, Mi Sook</au><au>Kang, Myung Joo</au><au>Lee, Do Ik</au><au>Joo, Seong Soo</au><au>Lee, Chung Soo</au><au>Bang, Hyoweon</au><au>Lee, Mi-Kyung</au><au>Myung, Soon-Chul</au><au>Choi, Young Wook</au><au>Lee, Kap-sok</au><au>Seo, Seong Jun</au><au>Lee, Min Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2010-08</date><risdate>2010</risdate><volume>19</volume><issue>8</issue><spage>e37</spage><epage>e43</epage><pages>e37-e43</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>Please cite this paper as: Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Experimental Dermatology 2010; 19: e37–e43.
: The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively in Korea, has been known to exert antioxidative, anti‐inflammatory, anti‐cancer and immune response inhibitory effects. The antioxidative effect of ORE was observed on the superoxide and 1,1‐diphenyl‐2‐picrylhydrazyl radical, as well as on the expression of inducible nitric oxide synthase and cyclooxygenase‐2 in lipopolysaccharide‐treated RAW264.7 macrophages. The statistically significant inhibitory action of ORE against production of cytokines induced by bacterial products or by interleukin (IL)‐1β, free radicals and nitrogen species, and a corresponding increase in cellular calcium concentration because of ORE were confirmed in bone marrow and spleen dendritic cells that are known to play important functions in the development and advancement of atopic dermatitis (AD). It was thus expected that ORE would exert a beneficial effect in the treatment of AD. A study on the pharmaceutical benefits of ORE against AD has not yet been conducted in vivo. We therefore used an in vivo AD animal model, namely the NC/Nga mice, and by applying ORE onto the animals through skin application as well as intraperitoneal injection, we attempted to evaluate the benefits of ORE in this system. Evaluation of ORE was conducted by following the SCORE method to score the effect, as well as by measuring the Th2 cytokines IL‐4, IL‐5 and IL‐13 levels from serum and lymphocytes, and IgE and eosinophil levels from serum. Additionally, the expression of mRNA and protein levels was estimated using real‐time polymerase chain reaction and Western blotting analysis. The following categories of clinical evaluation, Th2 cytokines IL‐4, IL‐5 and IL‐13 values, serum IgE levels, serum eosinophil levels, and mRNA and protein expression levels of iNOS and COX‐2, were evaluated from topical application and intraperitoneal injection groups of ORE. The effects of ORE on AD in NC/Nga mice were confirmed as being similar to the positive control group, while a significant difference with the negative control group was observed. The results presented in this report suggest that ORE might be beneficial in the treatment of AD.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19849716</pmid><doi>10.1111/j.1600-0625.2009.00961.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Administration, Topical Alnus Alnus japonica Animal models Animals Atopic dermatitis Bark Bone marrow Calcium Cyclooxygenase 2 - metabolism Cyclooxygenase-2 Cytokines - metabolism Dendritic cells Dermatitis, Atopic - drug therapy Dermatitis, Atopic - metabolism Dermatology diarylheptanoid Diarylheptanoids - administration & dosage Diarylheptanoids - therapeutic use Disease Models, Animal Female Free radicals Gene expression Helper cells Immune response Immunoglobulin E Immunoglobulin E - blood Inflammation Injections, Intraperitoneal Interleukin 13 Interleukin 4 Interleukin 5 Leukocytes (eosinophilic) Lymphocytes T Macrophages Mice Mice, Inbred Strains mRNA NC/Nga mice Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Nitrogen oregonin Pharmaceuticals Plant Extracts - administration & dosage Plant Extracts - therapeutic use Polymerase chain reaction Reactive Oxygen Species - metabolism Skin Spleen Statistical analysis Topical application Treatment Outcome Western blotting |
| title | Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice |
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