Non-apoptotic Cell Death Induced by Nutritional Starvation in J774.1 Mouse Macrophage-like Cell Line
The growth and amino acid utilization of a mouse macrophage-like cell line J774.1 was investigated in two different culture media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher rates in DMEM than in RPMI1640 medium. The consu...
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| Veröffentlicht in: | Anticancer research 2009-12, Vol.29 (12), p.5083-5088 |
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| creator | Thet, May Maw Yokote, Yoshiko Chu, Qing Wang, Qin-Tao Kishino, Kaori Tamura, Nozomi Shimada, Jun Amano, Osamu Kanda, Yumiko Kunii, Shiro Takekawa, Fumihiro Hatano, Hajime Shimada, Chiyako Hasegawa, Hideo Sakagami, Hiroshi |
| description | The growth and amino acid utilization of a mouse macrophage-like cell line J774.1 was investigated in two different culture
media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher
rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of
alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine,
was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3
activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines,
and DMEM is superior to RPMI1640 for long-term cell culture. |
| format | Article |
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media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher
rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of
alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine,
was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3
activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines,
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media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher
rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of
alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine,
was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3
activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines,
and DMEM is superior to RPMI1640 for long-term cell culture.</description><subject>Amino Acids - metabolism</subject><subject>Animals</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Death</subject><subject>Cells, Cultured</subject><subject>Enzyme Activation</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Mitochondria - metabolism</subject><subject>Starvation</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LlOw0AQBuAVApEQeAW0FVRGex8lCldQEgqgttb2EC_4Yr0G5e0xSqipRiN9-ufXHKAp1ZYmWnJyiKaESZJoQuQEnfT9OyFKWcOP0YQRIoRiZIqKddskrmu72Eaf4zlUFb4BF0u8aIohhwJnW7weYvDRt42r8HN04cv9Ltg3-FFrcUXxqh16wCuXh7Yr3QaSyn_ALmzpGzhFR2-u6uFsP2fo9e72Zf6QLJ_uF_PrZVIyzWOirOQZGMo5l0BlwXINmlCRE5YRagvDMrCKUSNya6ShipNMW26EU5kRmvAZutzldqH9HKCPae37fGzhGhgbploKozS37H_JxXhHUTrK870cshqKtAu-dmGb_r1wBBc7UPpN-e0DpH3tqmrkPHWB2ZSyVBLD-Q9Iu3eo</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Thet, May Maw</creator><creator>Yokote, Yoshiko</creator><creator>Chu, Qing</creator><creator>Wang, Qin-Tao</creator><creator>Kishino, Kaori</creator><creator>Tamura, Nozomi</creator><creator>Shimada, Jun</creator><creator>Amano, Osamu</creator><creator>Kanda, Yumiko</creator><creator>Kunii, Shiro</creator><creator>Takekawa, Fumihiro</creator><creator>Hatano, Hajime</creator><creator>Shimada, Chiyako</creator><creator>Hasegawa, Hideo</creator><creator>Sakagami, Hiroshi</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20091201</creationdate><title>Non-apoptotic Cell Death Induced by Nutritional Starvation in J774.1 Mouse Macrophage-like Cell Line</title><author>Thet, May Maw ; 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media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher
rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of
alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine,
was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3
activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines,
and DMEM is superior to RPMI1640 for long-term cell culture.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>20044620</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acids - metabolism Animals Caspase 3 - metabolism Cell Death Cells, Cultured Enzyme Activation Macrophages - cytology Macrophages - metabolism Mice Mitochondria - metabolism Starvation |
| title | Non-apoptotic Cell Death Induced by Nutritional Starvation in J774.1 Mouse Macrophage-like Cell Line |
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