Capillary Gas Chromatographic Measurement of Neutral Urinary Steroids: Evaluation and Comparison with Alternative Methodology
The major neutral urinary steroids have been quantitated by capillary gas chromatography and the reproducibility and accuracy of the method have been fully evaluated. Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, res...
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| Veröffentlicht in: | Annals of clinical biochemistry 1980-11, Vol.17 (6), p.319-322 |
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| container_title | Annals of clinical biochemistry |
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| creator | Phillipou, George Frith, R Gordon James, Steve K. |
| description | The major neutral urinary steroids have been quantitated by capillary gas chromatography and the reproducibility and accuracy of the method have been fully evaluated. Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, respectively. Comparison of steroid values with alternate and commonly employed procedures for measuring urinary 17-oxosteroids, total 17-oxogenic steroids, and cortisol and plasma dehydroepiandrosterone-sulphate yielded correlation coefficients between 0·71 and 0·79. A definite relationship between serum cortisol and urinary trihydroxy-pregnanedione was also shown. Urinary androstenedione metabolites, however, could not be significantly (P>0·05) correlated with the normalised androgen ratio in plasma. |
| doi_str_mv | 10.1177/000456328001700608 |
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Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, respectively. Comparison of steroid values with alternate and commonly employed procedures for measuring urinary 17-oxosteroids, total 17-oxogenic steroids, and cortisol and plasma dehydroepiandrosterone-sulphate yielded correlation coefficients between 0·71 and 0·79. A definite relationship between serum cortisol and urinary trihydroxy-pregnanedione was also shown. Urinary androstenedione metabolites, however, could not be significantly (P>0·05) correlated with the normalised androgen ratio in plasma.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/000456328001700608</identifier><identifier>PMID: 6452085</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>17-Hydroxycorticosteroids - urine ; 17-Ketosteroids - urine ; Chromatography, Gas ; Dehydroepiandrosterone - analogs & derivatives ; Dehydroepiandrosterone - blood ; Dehydroepiandrosterone Sulfate ; Humans ; Hydrocortisone - blood ; Male ; Pregnanes - urine ; Tetrahydrocortisone - urine</subject><ispartof>Annals of clinical biochemistry, 1980-11, Vol.17 (6), p.319-322</ispartof><rights>1980 Association for Clinical Biochemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-2cab7bd3a35a21ddabfec22004acdc427fbc157b00ee6daaf65e5838db4150553</citedby><cites>FETCH-LOGICAL-c382t-2cab7bd3a35a21ddabfec22004acdc427fbc157b00ee6daaf65e5838db4150553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6452085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phillipou, George</creatorcontrib><creatorcontrib>Frith, R Gordon</creatorcontrib><creatorcontrib>James, Steve K.</creatorcontrib><title>Capillary Gas Chromatographic Measurement of Neutral Urinary Steroids: Evaluation and Comparison with Alternative Methodology</title><title>Annals of clinical biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>The major neutral urinary steroids have been quantitated by capillary gas chromatography and the reproducibility and accuracy of the method have been fully evaluated. Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, respectively. Comparison of steroid values with alternate and commonly employed procedures for measuring urinary 17-oxosteroids, total 17-oxogenic steroids, and cortisol and plasma dehydroepiandrosterone-sulphate yielded correlation coefficients between 0·71 and 0·79. A definite relationship between serum cortisol and urinary trihydroxy-pregnanedione was also shown. Urinary androstenedione metabolites, however, could not be significantly (P>0·05) correlated with the normalised androgen ratio in plasma.</description><subject>17-Hydroxycorticosteroids - urine</subject><subject>17-Ketosteroids - urine</subject><subject>Chromatography, Gas</subject><subject>Dehydroepiandrosterone - analogs & derivatives</subject><subject>Dehydroepiandrosterone - blood</subject><subject>Dehydroepiandrosterone Sulfate</subject><subject>Humans</subject><subject>Hydrocortisone - blood</subject><subject>Male</subject><subject>Pregnanes - urine</subject><subject>Tetrahydrocortisone - urine</subject><issn>0004-5632</issn><issn>1758-1001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtL7TAUhYNc8R4ff0AQMrqzapI2ba8zKccH-Bio47Kb7J4TaZuapIoD_7sp5-BEcBR21rcWrEXIMWennBfFGWMsk3kqSsZ4wVjOyh2y4IUsEx5__pDFDCQz8Zfse_8STxG5PbKXZ1KwUi7IZwWj6TpwH_QKPK3WzvYQ7MrBuDaK3iH4yWGPQ6C2pfc4BQcdfXZmmC2PAZ012p_T5Rt0EwRjBwqDppXtR3DGx_PdhDW96CI5RP0NY2ZYW207u_o4JLstdB6Ptu8Beb5cPlXXye3D1U11cZuotBQhEQqaotEppBIE1xqaFpUQsRworTJRtI3ismgYQ8w1QJtLlGVa6ibjkkmZHpB_m9zR2dcJfah74xXG3gPaydeFzMr0fz6DYgMqZ7132NajM32sWnNWz5vXPzePppNt-tT0qL8t25GjfrbRPaywfrFTXKLzvyV-AenzjLE</recordid><startdate>198011</startdate><enddate>198011</enddate><creator>Phillipou, George</creator><creator>Frith, R Gordon</creator><creator>James, Steve K.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198011</creationdate><title>Capillary Gas Chromatographic Measurement of Neutral Urinary Steroids: Evaluation and Comparison with Alternative Methodology</title><author>Phillipou, George ; Frith, R Gordon ; James, Steve K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-2cab7bd3a35a21ddabfec22004acdc427fbc157b00ee6daaf65e5838db4150553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>17-Hydroxycorticosteroids - urine</topic><topic>17-Ketosteroids - urine</topic><topic>Chromatography, Gas</topic><topic>Dehydroepiandrosterone - analogs & derivatives</topic><topic>Dehydroepiandrosterone - blood</topic><topic>Dehydroepiandrosterone Sulfate</topic><topic>Humans</topic><topic>Hydrocortisone - blood</topic><topic>Male</topic><topic>Pregnanes - urine</topic><topic>Tetrahydrocortisone - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phillipou, George</creatorcontrib><creatorcontrib>Frith, R Gordon</creatorcontrib><creatorcontrib>James, Steve K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phillipou, George</au><au>Frith, R Gordon</au><au>James, Steve K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary Gas Chromatographic Measurement of Neutral Urinary Steroids: Evaluation and Comparison with Alternative Methodology</atitle><jtitle>Annals of clinical biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>1980-11</date><risdate>1980</risdate><volume>17</volume><issue>6</issue><spage>319</spage><epage>322</epage><pages>319-322</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><abstract>The major neutral urinary steroids have been quantitated by capillary gas chromatography and the reproducibility and accuracy of the method have been fully evaluated. Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, respectively. Comparison of steroid values with alternate and commonly employed procedures for measuring urinary 17-oxosteroids, total 17-oxogenic steroids, and cortisol and plasma dehydroepiandrosterone-sulphate yielded correlation coefficients between 0·71 and 0·79. A definite relationship between serum cortisol and urinary trihydroxy-pregnanedione was also shown. Urinary androstenedione metabolites, however, could not be significantly (P>0·05) correlated with the normalised androgen ratio in plasma.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>6452085</pmid><doi>10.1177/000456328001700608</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of clinical biochemistry, 1980-11, Vol.17 (6), p.319-322 |
| issn | 0004-5632 1758-1001 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 17-Hydroxycorticosteroids - urine 17-Ketosteroids - urine Chromatography, Gas Dehydroepiandrosterone - analogs & derivatives Dehydroepiandrosterone - blood Dehydroepiandrosterone Sulfate Humans Hydrocortisone - blood Male Pregnanes - urine Tetrahydrocortisone - urine |
| title | Capillary Gas Chromatographic Measurement of Neutral Urinary Steroids: Evaluation and Comparison with Alternative Methodology |
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