Two Interferon mRNAs in Human Fibroblasts: In vitro Translation and Escherichia coli Cloning Studies
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotid...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-12, Vol.77 (12), p.7152-7156 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 77 |
| creator | Weissenbach, Jean Chernajovsky, Yuti Zeevi, Menachem Shulman, Lester Soreq, Hermona Nir, Uri Wallach, David Perricaudet, Michel Tiollais, Pierre Revel, M. |
| description | Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mrprotein. The second interferon mRNA species (Hu IFN-β 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI· rC) but IFN-β 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-β 1 is not induced. |
| doi_str_mv | 10.1073/pnas.77.12.7152 |
| format | Article |
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The major fibroblast interferon (Hu IFN-β 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mrprotein. The second interferon mRNA species (Hu IFN-β 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI· rC) but IFN-β 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-β 1 is not induced.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.12.7152</identifier><identifier>PMID: 6164058</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Biological Assay ; Cell-Free System ; Cells, Cultured ; Cloning, Molecular - methods ; Complementary DNA ; DNA ; Escherichia coli - genetics ; Fibroblasts ; Gels ; Humans ; Interferons ; Interferons - genetics ; Messenger RNA ; Oocytes ; Plasmids ; Protein Biosynthesis ; Reticulocytes ; RNA ; RNA, Messenger - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-12, Vol.77 (12), p.7152-7156</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3752-e000eeb1bcd9f5318dbe250093bee715b578adda03b483f160b9bf1e58c8ae1a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/9720$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/9720$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6164058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weissenbach, Jean</creatorcontrib><creatorcontrib>Chernajovsky, Yuti</creatorcontrib><creatorcontrib>Zeevi, Menachem</creatorcontrib><creatorcontrib>Shulman, Lester</creatorcontrib><creatorcontrib>Soreq, Hermona</creatorcontrib><creatorcontrib>Nir, Uri</creatorcontrib><creatorcontrib>Wallach, David</creatorcontrib><creatorcontrib>Perricaudet, Michel</creatorcontrib><creatorcontrib>Tiollais, Pierre</creatorcontrib><creatorcontrib>Revel, M.</creatorcontrib><title>Two Interferon mRNAs in Human Fibroblasts: In vitro Translation and Escherichia coli Cloning Studies</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mrprotein. The second interferon mRNA species (Hu IFN-β 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI· rC) but IFN-β 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-β 1 is not induced.</description><subject>Biological Assay</subject><subject>Cell-Free System</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular - methods</subject><subject>Complementary DNA</subject><subject>DNA</subject><subject>Escherichia coli - genetics</subject><subject>Fibroblasts</subject><subject>Gels</subject><subject>Humans</subject><subject>Interferons</subject><subject>Interferons - genetics</subject><subject>Messenger RNA</subject><subject>Oocytes</subject><subject>Plasmids</subject><subject>Protein Biosynthesis</subject><subject>Reticulocytes</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFvEzEQhS1EVULhjDiAfILTpvZ6He8icaiillaqQIJwtmzvbOPKa6e2t9B_X0dJI3rhNIf3vZk3egi9o2ROiWCnG6_SXIg5reeC8voFmlHS0WrRdOQlmhFSi6pt6uYVep3SLSGk4y05RscLumgIb2eoX_0J-MpniAPE4PH48_tZwtbjy2lUHl9YHYN2KuX0pWD43uYY8Coqn5zKthiU7_F5MmuI1qytwiY4i5cueOtv8K889RbSG3Q0KJfg7X6eoN8X56vlZXX949vV8uy6MkzwuoKSD0BTbfpu4Iy2vYaal8xMA5TvNBet6ntFmG5aNtAF0Z0eKPDWtAqoYifo627vZtIj9AZ8jsrJTbSjig8yKCufK96u5U24l4yThnfF_2nvj-FugpTlaJMB55SHMCUpeCMYa0gBT3egiSGlCMPhBiVy24vc9iKFkLSW216K48O_0Q78voiif9zrW-OT-mzB5_8Ccpicy_A3F_L9jrxNOcQD2omasEeKB6xi</recordid><startdate>19801201</startdate><enddate>19801201</enddate><creator>Weissenbach, Jean</creator><creator>Chernajovsky, Yuti</creator><creator>Zeevi, Menachem</creator><creator>Shulman, Lester</creator><creator>Soreq, Hermona</creator><creator>Nir, Uri</creator><creator>Wallach, David</creator><creator>Perricaudet, Michel</creator><creator>Tiollais, Pierre</creator><creator>Revel, M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801201</creationdate><title>Two Interferon mRNAs in Human Fibroblasts: In vitro Translation and Escherichia coli Cloning Studies</title><author>Weissenbach, Jean ; Chernajovsky, Yuti ; Zeevi, Menachem ; Shulman, Lester ; Soreq, Hermona ; Nir, Uri ; Wallach, David ; Perricaudet, Michel ; Tiollais, Pierre ; Revel, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3752-e000eeb1bcd9f5318dbe250093bee715b578adda03b483f160b9bf1e58c8ae1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Biological Assay</topic><topic>Cell-Free System</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular - methods</topic><topic>Complementary DNA</topic><topic>DNA</topic><topic>Escherichia coli - genetics</topic><topic>Fibroblasts</topic><topic>Gels</topic><topic>Humans</topic><topic>Interferons</topic><topic>Interferons - genetics</topic><topic>Messenger RNA</topic><topic>Oocytes</topic><topic>Plasmids</topic><topic>Protein Biosynthesis</topic><topic>Reticulocytes</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weissenbach, Jean</creatorcontrib><creatorcontrib>Chernajovsky, Yuti</creatorcontrib><creatorcontrib>Zeevi, Menachem</creatorcontrib><creatorcontrib>Shulman, Lester</creatorcontrib><creatorcontrib>Soreq, Hermona</creatorcontrib><creatorcontrib>Nir, Uri</creatorcontrib><creatorcontrib>Wallach, David</creatorcontrib><creatorcontrib>Perricaudet, Michel</creatorcontrib><creatorcontrib>Tiollais, Pierre</creatorcontrib><creatorcontrib>Revel, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weissenbach, Jean</au><au>Chernajovsky, Yuti</au><au>Zeevi, Menachem</au><au>Shulman, Lester</au><au>Soreq, Hermona</au><au>Nir, Uri</au><au>Wallach, David</au><au>Perricaudet, Michel</au><au>Tiollais, Pierre</au><au>Revel, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two Interferon mRNAs in Human Fibroblasts: In vitro Translation and Escherichia coli Cloning Studies</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-12-01</date><risdate>1980</risdate><volume>77</volume><issue>12</issue><spage>7152</spage><epage>7156</epage><pages>7152-7156</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mrprotein. The second interferon mRNA species (Hu IFN-β 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI· rC) but IFN-β 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-β 1 is not induced.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6164058</pmid><doi>10.1073/pnas.77.12.7152</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Assay Cell-Free System Cells, Cultured Cloning, Molecular - methods Complementary DNA DNA Escherichia coli - genetics Fibroblasts Gels Humans Interferons Interferons - genetics Messenger RNA Oocytes Plasmids Protein Biosynthesis Reticulocytes RNA RNA, Messenger - genetics |
| title | Two Interferon mRNAs in Human Fibroblasts: In vitro Translation and Escherichia coli Cloning Studies |
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