Separation and quantitative analysis of rat testis histones by one-dimensional gel electrophoresis
A procedure is presented for direct separation and quantitative analysis of histones of rat testis by use of one-dimensional cylindrical gel electrophoresis in acid/urea/polyacrylamide gels at two concentrations of urea. After separation and staining with amido black the histone fractions are determ...
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| Veröffentlicht in: | Analytical biochemistry 1980-01, Vol.109 (1), p.102-108 |
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| container_title | Analytical biochemistry |
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| creator | Chiu, Marie L. Irvin, J.Logan |
| description | A procedure is presented for direct separation and quantitative analysis of histones of rat testis by use of one-dimensional cylindrical gel electrophoresis in acid/urea/polyacrylamide gels at two concentrations of urea. After separation and staining with amido black the histone fractions are determined by extraction of bound dye and spectrophotometric analysis. This method provides a rapid and accurate procedure for determination of microgram amounts of the histone subfractions which are unique to the testis as well as those which are found in somatic cells, and it is particularly useful for determination of molar proportions of the histones. |
| doi_str_mv | 10.1016/0003-2697(80)90016-0 |
| format | Article |
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After separation and staining with amido black the histone fractions are determined by extraction of bound dye and spectrophotometric analysis. 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After separation and staining with amido black the histone fractions are determined by extraction of bound dye and spectrophotometric analysis. This method provides a rapid and accurate procedure for determination of microgram amounts of the histone subfractions which are unique to the testis as well as those which are found in somatic cells, and it is particularly useful for determination of molar proportions of the histones.</description><subject>Amido Black</subject><subject>Animals</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Histones - analysis</subject><subject>Male</subject><subject>Rats</subject><subject>Testis - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UEtLxDAQDqLo-vgHCjmJHqrTtGnaiyCLLxA8qOeQpFM30m3WpCvsv3fWXTx6muR7TfIxdprDVQ55dQ0ARSaqRl3UcNkAQRnssEkODR0KaHbZ5E9ywA5T-iRNXspqn-2rsiJHNWH2FRcmmtGHgZuh5V9LM4x-JOAbCTD9KvnEQ8dJw0dMI91mPo1hwMTtitPMWj_HIVGC6fkH9hx7dGMMi1mISO5jtteZPuHJdh6x9_u7t-lj9vzy8DS9fc5cIdWYqUZ1beFsLSpZqKqC3HUdtqKzKIQthIDcSEeCQkhrG1ETWxtlLcjSConFETvf5C5i-FrSU_XcJ4d9bwYMy6SVLKWCuiZhuRG6GFKK2OlF9HMTVzoHva5Wr3vT6950Dfq3Wg1kO9vmL-0c2z_TtkvibzY80ie_PUadnMfBYesjFaLb4P9f8AMOvYpI</recordid><startdate>19800101</startdate><enddate>19800101</enddate><creator>Chiu, Marie L.</creator><creator>Irvin, J.Logan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800101</creationdate><title>Separation and quantitative analysis of rat testis histones by one-dimensional gel electrophoresis</title><author>Chiu, Marie L. ; Irvin, J.Logan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-797fd3cb8265376601cffed2fbe22b32201a5c3cb325bb928ffe8a7bb054b25e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Amido Black</topic><topic>Animals</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Histones - analysis</topic><topic>Male</topic><topic>Rats</topic><topic>Testis - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiu, Marie L.</creatorcontrib><creatorcontrib>Irvin, J.Logan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiu, Marie L.</au><au>Irvin, J.Logan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and quantitative analysis of rat testis histones by one-dimensional gel electrophoresis</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1980-01-01</date><risdate>1980</risdate><volume>109</volume><issue>1</issue><spage>102</spage><epage>108</epage><pages>102-108</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A procedure is presented for direct separation and quantitative analysis of histones of rat testis by use of one-dimensional cylindrical gel electrophoresis in acid/urea/polyacrylamide gels at two concentrations of urea. 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| ispartof | Analytical biochemistry, 1980-01, Vol.109 (1), p.102-108 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amido Black Animals Electrophoresis, Polyacrylamide Gel Histones - analysis Male Rats Testis - analysis |
| title | Separation and quantitative analysis of rat testis histones by one-dimensional gel electrophoresis |
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