Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses
Abstract Immunization against conserved virus components induces broad, heterosubtypic protection against diverse influenza A viruses, providing a strategy for controlling unexpected outbreaks or pandemics until strain-matched vaccines become available. This study characterized immunization to nucle...
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| Veröffentlicht in: | Vaccine 2009-11, Vol.27 (47), p.6512-6521 |
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| container_title | Vaccine |
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| creator | Price, Graeme E Soboleski, Mark R Lo, Chia-Yun Misplon, Julia A Pappas, Claudia Houser, Katherine V Tumpey, Terrence M Epstein, Suzanne L |
| description | Abstract Immunization against conserved virus components induces broad, heterosubtypic protection against diverse influenza A viruses, providing a strategy for controlling unexpected outbreaks or pandemics until strain-matched vaccines become available. This study characterized immunization to nucleoprotein (NP) and matrix 2 (M2) by DNA priming followed by parenteral or mucosal boosting in mice and ferrets. DNA vaccination followed by boosting with antigen-matched recombinant adenovirus (rAd) or cold-adapted ( ca ) influenza virus provided robust protection against virulent H1N1 and H5N1 challenges. Compared to other boosts, mucosal rAd induced stronger IgA responses, more virus-specific activated T-cells in the lung, and better protection against morbidity following challenge even eight months post-boost. In ferrets, both mucosal and parenteral rAd boosting protected from lethal H5N1 challenge. These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome. |
| doi_str_mv | 10.1016/j.vaccine.2009.08.053 |
| format | Article |
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This study characterized immunization to nucleoprotein (NP) and matrix 2 (M2) by DNA priming followed by parenteral or mucosal boosting in mice and ferrets. DNA vaccination followed by boosting with antigen-matched recombinant adenovirus (rAd) or cold-adapted ( ca ) influenza virus provided robust protection against virulent H1N1 and H5N1 challenges. Compared to other boosts, mucosal rAd induced stronger IgA responses, more virus-specific activated T-cells in the lung, and better protection against morbidity following challenge even eight months post-boost. In ferrets, both mucosal and parenteral rAd boosting protected from lethal H5N1 challenge. These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2009.08.053</identifier><identifier>PMID: 19729082</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adenovirus ; Adenoviruses ; Allergy and Immunology ; Animals ; Antibodies, Viral - blood ; Applied microbiology ; Avian flu ; Biological and medical sciences ; Catheters ; Cold-adapted ; Deoxyribonucleic acid ; DNA ; DNA vaccine ; Experiments ; Female ; Ferret ; Ferrets ; Fundamental and applied biological sciences. Psychology ; H5N1 ; Heterosubtypic immunity ; Immune response ; Immunity, Mucosal ; Immunization ; Immunization, Secondary ; Immunoglobulin A - blood ; Immunologic Memory ; Infections ; Influenza ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza A Virus, H5N1 Subtype - immunology ; Influenza Vaccines - immunology ; Influenza virus ; Intramuscular ; Intranasal ; Male ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mustela ; Orthomyxoviridae Infections - immunology ; Orthomyxoviridae Infections - prevention & control ; Pandemic ; Pandemics ; Peptides ; Prime-boost ; Public health ; Recombinant adenovirus ; RNA-Binding Proteins - immunology ; Systemic ; T-Lymphocytes - immunology ; Vaccine ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Viral Core Proteins - immunology ; Viral Matrix Proteins - immunology ; Virology ; Viruses</subject><ispartof>Vaccine, 2009-11, Vol.27 (47), p.6512-6521</ispartof><rights>2009</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Limited Nov 5, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c575t-258cf0518da41e636cdb2b7af42b607b766c7faa3a51758582eaeadc0b9421573</citedby><cites>FETCH-LOGICAL-c575t-258cf0518da41e636cdb2b7af42b607b766c7faa3a51758582eaeadc0b9421573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1618911766?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22174129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19729082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Price, Graeme E</creatorcontrib><creatorcontrib>Soboleski, Mark R</creatorcontrib><creatorcontrib>Lo, Chia-Yun</creatorcontrib><creatorcontrib>Misplon, Julia A</creatorcontrib><creatorcontrib>Pappas, Claudia</creatorcontrib><creatorcontrib>Houser, Katherine V</creatorcontrib><creatorcontrib>Tumpey, Terrence M</creatorcontrib><creatorcontrib>Epstein, Suzanne L</creatorcontrib><title>Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Immunization against conserved virus components induces broad, heterosubtypic protection against diverse influenza A viruses, providing a strategy for controlling unexpected outbreaks or pandemics until strain-matched vaccines become available. This study characterized immunization to nucleoprotein (NP) and matrix 2 (M2) by DNA priming followed by parenteral or mucosal boosting in mice and ferrets. DNA vaccination followed by boosting with antigen-matched recombinant adenovirus (rAd) or cold-adapted ( ca ) influenza virus provided robust protection against virulent H1N1 and H5N1 challenges. Compared to other boosts, mucosal rAd induced stronger IgA responses, more virus-specific activated T-cells in the lung, and better protection against morbidity following challenge even eight months post-boost. In ferrets, both mucosal and parenteral rAd boosting protected from lethal H5N1 challenge. These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome.</description><subject>Adenovirus</subject><subject>Adenoviruses</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Applied microbiology</subject><subject>Avian flu</subject><subject>Biological and medical sciences</subject><subject>Catheters</subject><subject>Cold-adapted</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA vaccine</subject><subject>Experiments</subject><subject>Female</subject><subject>Ferret</subject><subject>Ferrets</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>H5N1</subject><subject>Heterosubtypic immunity</subject><subject>Immune response</subject><subject>Immunity, Mucosal</subject><subject>Immunization</subject><subject>Immunization, Secondary</subject><subject>Immunoglobulin A - blood</subject><subject>Immunologic Memory</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A Virus, H1N1 Subtype - immunology</subject><subject>Influenza A Virus, H5N1 Subtype - immunology</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza virus</subject><subject>Intramuscular</subject><subject>Intranasal</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mustela</subject><subject>Orthomyxoviridae Infections - immunology</subject><subject>Orthomyxoviridae Infections - prevention & control</subject><subject>Pandemic</subject><subject>Pandemics</subject><subject>Peptides</subject><subject>Prime-boost</subject><subject>Public health</subject><subject>Recombinant adenovirus</subject><subject>RNA-Binding Proteins - immunology</subject><subject>Systemic</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccine</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Viral Core Proteins - immunology</subject><subject>Viral Matrix Proteins - immunology</subject><subject>Virology</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk2LFDEQhoMo7rj6E5SAiKcZU-nOR1-UZVFHWPTgB95COl09ZOxOr0n3wHjan76ZmcaFvewpoXiq8r55i5CXwFbAQL7brnbWOR9wxRmrVkyvmCgekQVoVSy5AP2YLBiX5bIE9vuMPEtpy1hGoHpKzqBSvGKaL8jNr-MUO_oh0HZwU_JhQ33fT8GPe5qLbggJ4w4basPoNxgSvY7DiG5MtPcOc7mhLcaIuWA31oc00p2PU4dhpGv4CkdiLfLFh7abMPyz9OKIJEzPyZPWdglfzOc5-fnp44_L9fLq2-cvlxdXSyeUGLMj7VqWbTW2BJSFdE3Na2XbkteSqVpJ6VRrbWEFKKGF5mjRNo7VVclBqOKcvD3NzeL_TphG0_vksOtswGFKRolSSFmCfJgsSuDAK53J1_fI7TDFkG0YkKArgCwrU-JEuTikFLE119H3Nu4NMHPI0mzNnKU5ZGmYNjmo3Pdqnj7VPTZ3XXN4GXgzAzY527XRBufTf45zUFlplbkPJw7z_-48RpOcx-Cw8THHaJrBPyjl_b0JrvPB50f_4B7TnWuTuGHm-2HxDnvHKga8ZKq4Bbf41Kw</recordid><startdate>20091105</startdate><enddate>20091105</enddate><creator>Price, Graeme E</creator><creator>Soboleski, Mark R</creator><creator>Lo, Chia-Yun</creator><creator>Misplon, Julia A</creator><creator>Pappas, Claudia</creator><creator>Houser, Katherine V</creator><creator>Tumpey, Terrence M</creator><creator>Epstein, Suzanne L</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20091105</creationdate><title>Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses</title><author>Price, Graeme E ; Soboleski, Mark R ; Lo, Chia-Yun ; Misplon, Julia A ; Pappas, Claudia ; Houser, Katherine V ; Tumpey, Terrence M ; Epstein, Suzanne L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-258cf0518da41e636cdb2b7af42b607b766c7faa3a51758582eaeadc0b9421573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenovirus</topic><topic>Adenoviruses</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Applied microbiology</topic><topic>Avian flu</topic><topic>Biological and medical sciences</topic><topic>Catheters</topic><topic>Cold-adapted</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA vaccine</topic><topic>Experiments</topic><topic>Female</topic><topic>Ferret</topic><topic>Ferrets</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>H5N1</topic><topic>Heterosubtypic immunity</topic><topic>Immune response</topic><topic>Immunity, Mucosal</topic><topic>Immunization</topic><topic>Immunization, Secondary</topic><topic>Immunoglobulin A - blood</topic><topic>Immunologic Memory</topic><topic>Infections</topic><topic>Influenza</topic><topic>Influenza A Virus, H1N1 Subtype - immunology</topic><topic>Influenza A Virus, H5N1 Subtype - immunology</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza virus</topic><topic>Intramuscular</topic><topic>Intranasal</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mustela</topic><topic>Orthomyxoviridae Infections - immunology</topic><topic>Orthomyxoviridae Infections - prevention & control</topic><topic>Pandemic</topic><topic>Pandemics</topic><topic>Peptides</topic><topic>Prime-boost</topic><topic>Public health</topic><topic>Recombinant adenovirus</topic><topic>RNA-Binding Proteins - immunology</topic><topic>Systemic</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccine</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Viral Core Proteins - immunology</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Price, Graeme E</creatorcontrib><creatorcontrib>Soboleski, Mark R</creatorcontrib><creatorcontrib>Lo, Chia-Yun</creatorcontrib><creatorcontrib>Misplon, Julia A</creatorcontrib><creatorcontrib>Pappas, Claudia</creatorcontrib><creatorcontrib>Houser, Katherine V</creatorcontrib><creatorcontrib>Tumpey, Terrence M</creatorcontrib><creatorcontrib>Epstein, Suzanne L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Price, Graeme E</au><au>Soboleski, Mark R</au><au>Lo, Chia-Yun</au><au>Misplon, Julia A</au><au>Pappas, Claudia</au><au>Houser, Katherine V</au><au>Tumpey, Terrence M</au><au>Epstein, Suzanne L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2009-11-05</date><risdate>2009</risdate><volume>27</volume><issue>47</issue><spage>6512</spage><epage>6521</epage><pages>6512-6521</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Immunization against conserved virus components induces broad, heterosubtypic protection against diverse influenza A viruses, providing a strategy for controlling unexpected outbreaks or pandemics until strain-matched vaccines become available. This study characterized immunization to nucleoprotein (NP) and matrix 2 (M2) by DNA priming followed by parenteral or mucosal boosting in mice and ferrets. DNA vaccination followed by boosting with antigen-matched recombinant adenovirus (rAd) or cold-adapted ( ca ) influenza virus provided robust protection against virulent H1N1 and H5N1 challenges. Compared to other boosts, mucosal rAd induced stronger IgA responses, more virus-specific activated T-cells in the lung, and better protection against morbidity following challenge even eight months post-boost. In ferrets, both mucosal and parenteral rAd boosting protected from lethal H5N1 challenge. These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19729082</pmid><doi>10.1016/j.vaccine.2009.08.053</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenovirus Adenoviruses Allergy and Immunology Animals Antibodies, Viral - blood Applied microbiology Avian flu Biological and medical sciences Catheters Cold-adapted Deoxyribonucleic acid DNA DNA vaccine Experiments Female Ferret Ferrets Fundamental and applied biological sciences. Psychology H5N1 Heterosubtypic immunity Immune response Immunity, Mucosal Immunization Immunization, Secondary Immunoglobulin A - blood Immunologic Memory Infections Influenza Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H5N1 Subtype - immunology Influenza Vaccines - immunology Influenza virus Intramuscular Intranasal Male Mice Mice, Inbred BALB C Microbiology Miscellaneous Mustela Orthomyxoviridae Infections - immunology Orthomyxoviridae Infections - prevention & control Pandemic Pandemics Peptides Prime-boost Public health Recombinant adenovirus RNA-Binding Proteins - immunology Systemic T-Lymphocytes - immunology Vaccine Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral Core Proteins - immunology Viral Matrix Proteins - immunology Virology Viruses |
| title | Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses |
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