Long-term presence of memory B-cells specific for different vaccine components
Abstract Vaccination against infectious diseases ideally should provide lifelong immunity, but in many cases waning of antibody titers has been observed over time. In this study we describe the identification of antigen-specific memory B-cells in peripheral blood of persons born between 1940 and 200...
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| Veröffentlicht in: | Vaccine 2009-12, Vol.28 (1), p.179-186 |
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| creator | Buisman, A.M de Rond, C.G.H Öztürk, K ten Hulscher, H.I van Binnendijk, R.S |
| description | Abstract Vaccination against infectious diseases ideally should provide lifelong immunity, but in many cases waning of antibody titers has been observed over time. In this study we describe the identification of antigen-specific memory B-cells in peripheral blood of persons born between 1940 and 2004 in The Netherlands. Polyclonal stimulation of either PBMCs or purified B-cells induced proliferation and differentiation of B-cells of the memory phenotype (CD19+ /CD27+ ) into antibody secreting cells (ASC). Memory B-cells against components of bacterial vaccines ( Bordetella pertussis and tetanus) as well as viral vaccines (measles and influenza) were thus identified, even in persons with low serum antibody titers. Enrichment of B-cells increased the sensitivity of memory B-cell detection when compared to PBMCs. Low, but significant correlations between numbers of antigen-specific memory B-cells and the corresponding circulating antibody titers were found for the pertussis proteins and measles virus, but not for tetanus. The identification of the numbers and specificities of peripheral memory B-cells and their relationship with circulating antibodies may be very useful to determine the long-term efficacy of vaccination. |
| doi_str_mv | 10.1016/j.vaccine.2009.09.102 |
| format | Article |
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In this study we describe the identification of antigen-specific memory B-cells in peripheral blood of persons born between 1940 and 2004 in The Netherlands. Polyclonal stimulation of either PBMCs or purified B-cells induced proliferation and differentiation of B-cells of the memory phenotype (CD19+ /CD27+ ) into antibody secreting cells (ASC). Memory B-cells against components of bacterial vaccines ( Bordetella pertussis and tetanus) as well as viral vaccines (measles and influenza) were thus identified, even in persons with low serum antibody titers. Enrichment of B-cells increased the sensitivity of memory B-cell detection when compared to PBMCs. Low, but significant correlations between numbers of antigen-specific memory B-cells and the corresponding circulating antibody titers were found for the pertussis proteins and measles virus, but not for tetanus. The identification of the numbers and specificities of peripheral memory B-cells and their relationship with circulating antibodies may be very useful to determine the long-term efficacy of vaccination.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2009.09.102</identifier><identifier>PMID: 19799844</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adult ; Age ; Age groups ; Aged ; Allergy and Immunology ; Antibodies ; Antibodies, Bacterial - blood ; Antibodies, Viral - blood ; B-Lymphocytes - immunology ; Bordetella pertussis ; Cell Differentiation ; Cell Proliferation ; Child ; Cohort Studies ; Cytokines ; Diphtheria-Tetanus-Pertussis Vaccine - immunology ; Disease ; ELIspot ; Humans ; Immune system ; Immunoglobulin G - blood ; Immunologic Memory ; Infections ; Infectious diseases ; Leukocytes, Mononuclear - immunology ; Lymphocyte Activation ; Measles ; Measles Vaccine - immunology ; Memory B-cell ; Middle Aged ; Pertussis ; Plasma ; Public health ; Sensitivity and Specificity ; Studies ; Tetanus ; Vaccination ; Vaccines ; Whooping cough</subject><ispartof>Vaccine, 2009-12, Vol.28 (1), p.179-186</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><rights>Copyright Elsevier Limited Dec 10, 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-f012cadb11298796832501a9fd8fd8c654e906c9ccb8151e7b7e375d991c25bb3</citedby><cites>FETCH-LOGICAL-c526t-f012cadb11298796832501a9fd8fd8c654e906c9ccb8151e7b7e375d991c25bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1618921832?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19799844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buisman, A.M</creatorcontrib><creatorcontrib>de Rond, C.G.H</creatorcontrib><creatorcontrib>Öztürk, K</creatorcontrib><creatorcontrib>ten Hulscher, H.I</creatorcontrib><creatorcontrib>van Binnendijk, R.S</creatorcontrib><title>Long-term presence of memory B-cells specific for different vaccine components</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Vaccination against infectious diseases ideally should provide lifelong immunity, but in many cases waning of antibody titers has been observed over time. In this study we describe the identification of antigen-specific memory B-cells in peripheral blood of persons born between 1940 and 2004 in The Netherlands. Polyclonal stimulation of either PBMCs or purified B-cells induced proliferation and differentiation of B-cells of the memory phenotype (CD19+ /CD27+ ) into antibody secreting cells (ASC). Memory B-cells against components of bacterial vaccines ( Bordetella pertussis and tetanus) as well as viral vaccines (measles and influenza) were thus identified, even in persons with low serum antibody titers. Enrichment of B-cells increased the sensitivity of memory B-cell detection when compared to PBMCs. Low, but significant correlations between numbers of antigen-specific memory B-cells and the corresponding circulating antibody titers were found for the pertussis proteins and measles virus, but not for tetanus. The identification of the numbers and specificities of peripheral memory B-cells and their relationship with circulating antibodies may be very useful to determine the long-term efficacy of vaccination.</description><subject>Adult</subject><subject>Age</subject><subject>Age groups</subject><subject>Aged</subject><subject>Allergy and Immunology</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibodies, Viral - blood</subject><subject>B-Lymphocytes - immunology</subject><subject>Bordetella pertussis</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Child</subject><subject>Cohort Studies</subject><subject>Cytokines</subject><subject>Diphtheria-Tetanus-Pertussis Vaccine - immunology</subject><subject>Disease</subject><subject>ELIspot</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunoglobulin G - blood</subject><subject>Immunologic Memory</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocyte Activation</subject><subject>Measles</subject><subject>Measles Vaccine - immunology</subject><subject>Memory B-cell</subject><subject>Middle Aged</subject><subject>Pertussis</subject><subject>Plasma</subject><subject>Public health</subject><subject>Sensitivity and Specificity</subject><subject>Studies</subject><subject>Tetanus</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Whooping cough</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFksFq3DAQhkVpaTZpH6HF0ENP3mhkybYuLWlo2sDSHNJCb8Iej4q2tuVK3sC-fWXWEMglMDAgvvk1M_8w9g74FjiUl_vtQ4PoRtoKzvU2BXDxgm2gropcKKhfsg0Xpcwl8N9n7DzGPedcFaBfszPQlda1lBv2Y-fHP_lMYcimQJFGpMzbbKDBh2P2JUfq-5jFidBZh5n1IeuctRRonLO1gwz9MPkxvcQ37JVt-khv13zBft18_Xn9Pd_dfbu9vtrlqEQ555aDwKZrAYSuK13WhVAcGm27OgWWSpLmJWrEtgYFVLUVFZXqtAYUqm2LC_bxpDsF_-9AcTaDi0uvzUj-EE2lpCqTJDxPFhJKySudyA9PyL0_hDGNYaCEWgtIbSZKnSgMPsZA1kzBDU04GuBmccbszboXszhjUiRnUt37Vf3QDtQ9Vq1WJODzCaC0twdHwUR0ix-dC4Sz6bx79otPTxSwd6PDpv9LR4qP05goDDf3y3ks18E1Bym1KP4DVnu1eg</recordid><startdate>20091210</startdate><enddate>20091210</enddate><creator>Buisman, A.M</creator><creator>de Rond, C.G.H</creator><creator>Öztürk, K</creator><creator>ten Hulscher, H.I</creator><creator>van Binnendijk, R.S</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20091210</creationdate><title>Long-term presence of memory B-cells specific for different vaccine components</title><author>Buisman, A.M ; de Rond, C.G.H ; Öztürk, K ; ten Hulscher, H.I ; van Binnendijk, R.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-f012cadb11298796832501a9fd8fd8c654e906c9ccb8151e7b7e375d991c25bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Age</topic><topic>Age groups</topic><topic>Aged</topic><topic>Allergy and Immunology</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buisman, A.M</au><au>de Rond, C.G.H</au><au>Öztürk, K</au><au>ten Hulscher, H.I</au><au>van Binnendijk, R.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long-term presence of memory B-cells specific for different vaccine components</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2009-12-10</date><risdate>2009</risdate><volume>28</volume><issue>1</issue><spage>179</spage><epage>186</epage><pages>179-186</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Abstract Vaccination against infectious diseases ideally should provide lifelong immunity, but in many cases waning of antibody titers has been observed over time. In this study we describe the identification of antigen-specific memory B-cells in peripheral blood of persons born between 1940 and 2004 in The Netherlands. Polyclonal stimulation of either PBMCs or purified B-cells induced proliferation and differentiation of B-cells of the memory phenotype (CD19+ /CD27+ ) into antibody secreting cells (ASC). Memory B-cells against components of bacterial vaccines ( Bordetella pertussis and tetanus) as well as viral vaccines (measles and influenza) were thus identified, even in persons with low serum antibody titers. Enrichment of B-cells increased the sensitivity of memory B-cell detection when compared to PBMCs. Low, but significant correlations between numbers of antigen-specific memory B-cells and the corresponding circulating antibody titers were found for the pertussis proteins and measles virus, but not for tetanus. The identification of the numbers and specificities of peripheral memory B-cells and their relationship with circulating antibodies may be very useful to determine the long-term efficacy of vaccination.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19799844</pmid><doi>10.1016/j.vaccine.2009.09.102</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Age Age groups Aged Allergy and Immunology Antibodies Antibodies, Bacterial - blood Antibodies, Viral - blood B-Lymphocytes - immunology Bordetella pertussis Cell Differentiation Cell Proliferation Child Cohort Studies Cytokines Diphtheria-Tetanus-Pertussis Vaccine - immunology Disease ELIspot Humans Immune system Immunoglobulin G - blood Immunologic Memory Infections Infectious diseases Leukocytes, Mononuclear - immunology Lymphocyte Activation Measles Measles Vaccine - immunology Memory B-cell Middle Aged Pertussis Plasma Public health Sensitivity and Specificity Studies Tetanus Vaccination Vaccines Whooping cough |
| title | Long-term presence of memory B-cells specific for different vaccine components |
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