Development of candidate combination vaccine for hepatitis E and hepatitis B: A liposome encapsulation approach
Abstract To reduce extra injections, cost and ensure better coverage, use of combination vaccines is preferable. An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DN...
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| Veröffentlicht in: | Vaccine 2009-11, Vol.27 (47), p.6582-6588 |
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| creator | Shrivastava, Shubham Lole, Kavita S Tripathy, Anuradha S Shaligram, Umesh S Arankalle, Vidya A |
| description | Abstract To reduce extra injections, cost and ensure better coverage, use of combination vaccines is preferable. An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DNA + protein. Mice groups were immunized with different liposome-encapsulated formulations and monitored for anti-HEV and anti-HBs titres, IgG subtypes, antigen-specific lymphocyte proliferation and cytokine levels. The protective levels of anti-HBs and in vitro virus-binding capacity of anti-HEV antibodies were assessed. Liposome-encapsulated DNA either singly or in combination did not elicit antibody response. Anti-HEV and anti-HBs IgG titres of individual component of protein alone (Lipo-E-P/Lipo-B-P) or DNA + protein formulations (Lipo-E-DP/Lipo-B-DP) were comparable to respective titres in combination vaccine of protein (Lipo-BE-P) and DNA + protein formulations (Lipo-BE-DP). IgG1 levels were significantly higher in Lipo-BE-P group whereas, equivalent levels of IgG1 and IgG2a were observed in Lipo-BE-DP group against both components of the vaccine. Combination vaccine group showed mixed Th1/Th2 cytokine profile. Liposome entrapped NE and HBsAg in protein and DNA + protein formats induce excellent immune response to both the components and need to be evaluated in higher animals. |
| doi_str_mv | 10.1016/j.vaccine.2009.08.033 |
| format | Article |
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An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DNA + protein. Mice groups were immunized with different liposome-encapsulated formulations and monitored for anti-HEV and anti-HBs titres, IgG subtypes, antigen-specific lymphocyte proliferation and cytokine levels. The protective levels of anti-HBs and in vitro virus-binding capacity of anti-HEV antibodies were assessed. Liposome-encapsulated DNA either singly or in combination did not elicit antibody response. Anti-HEV and anti-HBs IgG titres of individual component of protein alone (Lipo-E-P/Lipo-B-P) or DNA + protein formulations (Lipo-E-DP/Lipo-B-DP) were comparable to respective titres in combination vaccine of protein (Lipo-BE-P) and DNA + protein formulations (Lipo-BE-DP). IgG1 levels were significantly higher in Lipo-BE-P group whereas, equivalent levels of IgG1 and IgG2a were observed in Lipo-BE-DP group against both components of the vaccine. Combination vaccine group showed mixed Th1/Th2 cytokine profile. Liposome entrapped NE and HBsAg in protein and DNA + protein formats induce excellent immune response to both the components and need to be evaluated in higher animals.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2009.08.033</identifier><identifier>PMID: 19747579</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; Antibody Formation ; Antigens ; Applied microbiology ; Biological and medical sciences ; Cell Proliferation ; Cloning ; Co-delivery approach ; Combination vaccine ; Cytokines ; Cytokines - immunology ; Deoxyribonucleic acid ; DNA ; Female ; Fundamental and applied biological sciences. Psychology ; Hepatitis ; Hepatitis Antibodies - blood ; Hepatitis B ; Hepatitis B - immunology ; Hepatitis B - prevention & control ; Hepatitis B Surface Antigens - immunology ; Hepatitis B virus ; Hepatitis E ; Hepatitis E - immunology ; Hepatitis E - prevention & control ; Hepatitis E virus ; Human viral diseases ; Immune response ; Immunity, Cellular ; Immunization ; Immunoglobulin G - blood ; Infectious diseases ; Liposome ; Liposomes - immunology ; Lymphocytes ; Medical sciences ; Mice ; Microbiology ; Plasmids ; Proteins ; Recombinant Proteins - immunology ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Combined - immunology ; Vaccines, DNA - immunology ; Viral diseases ; Viral hepatitis ; Viral Hepatitis Vaccines - immunology ; Viruses</subject><ispartof>Vaccine, 2009-11, Vol.27 (47), p.6582-6588</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Limited Nov 5, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-d5854cb4bfdff0a2ebb32bb060a8bce13c0633c7b264041a17e64f0644a05fa43</citedby><cites>FETCH-LOGICAL-c509t-d5854cb4bfdff0a2ebb32bb060a8bce13c0633c7b264041a17e64f0644a05fa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X09012080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22174139$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19747579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shrivastava, Shubham</creatorcontrib><creatorcontrib>Lole, Kavita S</creatorcontrib><creatorcontrib>Tripathy, Anuradha S</creatorcontrib><creatorcontrib>Shaligram, Umesh S</creatorcontrib><creatorcontrib>Arankalle, Vidya A</creatorcontrib><title>Development of candidate combination vaccine for hepatitis E and hepatitis B: A liposome encapsulation approach</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract To reduce extra injections, cost and ensure better coverage, use of combination vaccines is preferable. An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DNA + protein. Mice groups were immunized with different liposome-encapsulated formulations and monitored for anti-HEV and anti-HBs titres, IgG subtypes, antigen-specific lymphocyte proliferation and cytokine levels. The protective levels of anti-HBs and in vitro virus-binding capacity of anti-HEV antibodies were assessed. Liposome-encapsulated DNA either singly or in combination did not elicit antibody response. Anti-HEV and anti-HBs IgG titres of individual component of protein alone (Lipo-E-P/Lipo-B-P) or DNA + protein formulations (Lipo-E-DP/Lipo-B-DP) were comparable to respective titres in combination vaccine of protein (Lipo-BE-P) and DNA + protein formulations (Lipo-BE-DP). IgG1 levels were significantly higher in Lipo-BE-P group whereas, equivalent levels of IgG1 and IgG2a were observed in Lipo-BE-DP group against both components of the vaccine. Combination vaccine group showed mixed Th1/Th2 cytokine profile. Liposome entrapped NE and HBsAg in protein and DNA + protein formats induce excellent immune response to both the components and need to be evaluated in higher animals.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Cloning</subject><subject>Co-delivery approach</subject><subject>Combination vaccine</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis</subject><subject>Hepatitis Antibodies - blood</subject><subject>Hepatitis B</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B - prevention & control</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B virus</subject><subject>Hepatitis E</subject><subject>Hepatitis E - immunology</subject><subject>Hepatitis E - prevention & control</subject><subject>Hepatitis E virus</subject><subject>Human viral diseases</subject><subject>Immune response</subject><subject>Immunity, Cellular</subject><subject>Immunization</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious diseases</subject><subject>Liposome</subject><subject>Liposomes - immunology</subject><subject>Lymphocytes</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Recombinant Proteins - immunology</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Combined - immunology</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><subject>Viral Hepatitis Vaccines - immunology</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkl2L1TAQhoso7tnVn6AERLxqnTRp03qhrOv6AQteqOBdSNIJm2Ob1KY9sP9-c2hxZW_2Kkx45p2Pd7LsBYWCAq3f7ouDMsZ5LEqAtoCmAMYeZTvaCJaXFW0eZzsoa55zCr9PstMY9wBQMdo-zU5oK7ioRLvLwic8YB_GAf1MgiVG-c51akZiwqCdV7MLnmyliA0TucYxfc4ukkuS4P_ij-_IOendGGIYkKA3aoxLvyqocZyCMtfPsidW9RGfb-9Z9uvz5c-Lr_nV9y_fLs6vclNBO-dd1VTcaK5tZy2oErVmpdZQg2q0QcoM1IwZodOEwKmiAmtuoeZcQWUVZ2fZm1U3lf27YJzl4KLBvlcewxKlqHhV81bQh0nGaVlS1iby1T1yH5bJpzEkrWnT0hJqkahqpcwUYpzQynFyg5puJAV5tE7u5bZPebROQiOTdSnv5aa-6AG7u6zNqwS83gAVjertpLxx8R-XehR8bfPDymHa78HhJKNxyQ3s3IRmll1wD7by_p6C6Z13qegfvMF4N7WMpQT543hnxzODFtIOGmC3l1HO7w</recordid><startdate>20091105</startdate><enddate>20091105</enddate><creator>Shrivastava, Shubham</creator><creator>Lole, Kavita S</creator><creator>Tripathy, Anuradha S</creator><creator>Shaligram, Umesh S</creator><creator>Arankalle, Vidya A</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20091105</creationdate><title>Development of candidate combination vaccine for hepatitis E and hepatitis B: A liposome encapsulation approach</title><author>Shrivastava, Shubham ; Lole, Kavita S ; Tripathy, Anuradha S ; Shaligram, Umesh S ; Arankalle, Vidya A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-d5854cb4bfdff0a2ebb32bb060a8bce13c0633c7b264041a17e64f0644a05fa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Cloning</topic><topic>Co-delivery approach</topic><topic>Combination vaccine</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepatitis</topic><topic>Hepatitis Antibodies - blood</topic><topic>Hepatitis B</topic><topic>Hepatitis B - immunology</topic><topic>Hepatitis B - prevention & control</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B virus</topic><topic>Hepatitis E</topic><topic>Hepatitis E - immunology</topic><topic>Hepatitis E - prevention & control</topic><topic>Hepatitis E virus</topic><topic>Human viral diseases</topic><topic>Immune response</topic><topic>Immunity, Cellular</topic><topic>Immunization</topic><topic>Immunoglobulin G - blood</topic><topic>Infectious diseases</topic><topic>Liposome</topic><topic>Liposomes - immunology</topic><topic>Lymphocytes</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Recombinant Proteins - immunology</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, Combined - 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An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DNA + protein. Mice groups were immunized with different liposome-encapsulated formulations and monitored for anti-HEV and anti-HBs titres, IgG subtypes, antigen-specific lymphocyte proliferation and cytokine levels. The protective levels of anti-HBs and in vitro virus-binding capacity of anti-HEV antibodies were assessed. Liposome-encapsulated DNA either singly or in combination did not elicit antibody response. Anti-HEV and anti-HBs IgG titres of individual component of protein alone (Lipo-E-P/Lipo-B-P) or DNA + protein formulations (Lipo-E-DP/Lipo-B-DP) were comparable to respective titres in combination vaccine of protein (Lipo-BE-P) and DNA + protein formulations (Lipo-BE-DP). IgG1 levels were significantly higher in Lipo-BE-P group whereas, equivalent levels of IgG1 and IgG2a were observed in Lipo-BE-DP group against both components of the vaccine. Combination vaccine group showed mixed Th1/Th2 cytokine profile. Liposome entrapped NE and HBsAg in protein and DNA + protein formats induce excellent immune response to both the components and need to be evaluated in higher animals.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19747579</pmid><doi>10.1016/j.vaccine.2009.08.033</doi><tpages>7</tpages></addata></record> |
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| ispartof | Vaccine, 2009-11, Vol.27 (47), p.6582-6588 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Allergy and Immunology Animals Antibody Formation Antigens Applied microbiology Biological and medical sciences Cell Proliferation Cloning Co-delivery approach Combination vaccine Cytokines Cytokines - immunology Deoxyribonucleic acid DNA Female Fundamental and applied biological sciences. Psychology Hepatitis Hepatitis Antibodies - blood Hepatitis B Hepatitis B - immunology Hepatitis B - prevention & control Hepatitis B Surface Antigens - immunology Hepatitis B virus Hepatitis E Hepatitis E - immunology Hepatitis E - prevention & control Hepatitis E virus Human viral diseases Immune response Immunity, Cellular Immunization Immunoglobulin G - blood Infectious diseases Liposome Liposomes - immunology Lymphocytes Medical sciences Mice Microbiology Plasmids Proteins Recombinant Proteins - immunology Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Combined - immunology Vaccines, DNA - immunology Viral diseases Viral hepatitis Viral Hepatitis Vaccines - immunology Viruses |
| title | Development of candidate combination vaccine for hepatitis E and hepatitis B: A liposome encapsulation approach |
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