Hypermethylation of 5'-region of the human calcitonin gene in leukemias: structural features and diagnostic significance
Methylation of the 5'-region of the calcitonin gene was investigated in bone marrow and peripheral blood cells of 27 healthy volunteers and 25 leukemic patients. In all patients suffering from various forms of myeloid and lymphoid leukemia, hypermethylation of CpG sequences was observed in this...
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| Veröffentlicht in: | Biochemistry (Moscow) 2004-03, Vol.69 (3), p.340-349 |
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| creator | Marinitch, D V Vorobyev, I A Holmes, J A Zakharchenko, N S Dyachenko, O V Buryanov, Ya I Shevchuk, T V |
| description | Methylation of the 5'-region of the calcitonin gene was investigated in bone marrow and peripheral blood cells of 27 healthy volunteers and 25 leukemic patients. In all patients suffering from various forms of myeloid and lymphoid leukemia, hypermethylation of CpG sequences was observed in this region of the calcitonin gene. Cytosine hypermethylation in the CpG sequence did not involve cytosines of adjacent CpNpG sequences (where N is any nucleoside). The 5'-region of the calcitonin gene lacked CpNpG methylation both in healthy controls and in leukemic patients; this apparently represents specific "non-alternative" type of CpG methylation in the extended DNA sequence. Methylation of the calcitonin gene was monitored in 18 leukemic patients during malignant progression and medical treatment. Hypermethylation of the calcitonin gene was not observed on long-term clinical hematological remission. In ten patients characterized by unstable (or incomplete) remission hypermethylation of the calcitonin gene persisted through the whole period of observation. In relapses, hypermethylation of the calcitonin gene appeared again and in six patients, this "molecular relapse" being registered 1-8 months before onset of clinical and laboratory signs of disease progression. The leukemia-specific hypermethylation of CpG sequences of the 5'-region of the calcitonin gene is a promising prognostic and diagnostic marker of leukemias and might be useful for monitoring of this disease. |
| doi_str_mv | 10.1023/B:BIRY.0000022067.63532.b8 |
| format | Article |
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In all patients suffering from various forms of myeloid and lymphoid leukemia, hypermethylation of CpG sequences was observed in this region of the calcitonin gene. Cytosine hypermethylation in the CpG sequence did not involve cytosines of adjacent CpNpG sequences (where N is any nucleoside). The 5'-region of the calcitonin gene lacked CpNpG methylation both in healthy controls and in leukemic patients; this apparently represents specific "non-alternative" type of CpG methylation in the extended DNA sequence. Methylation of the calcitonin gene was monitored in 18 leukemic patients during malignant progression and medical treatment. Hypermethylation of the calcitonin gene was not observed on long-term clinical hematological remission. In ten patients characterized by unstable (or incomplete) remission hypermethylation of the calcitonin gene persisted through the whole period of observation. In relapses, hypermethylation of the calcitonin gene appeared again and in six patients, this "molecular relapse" being registered 1-8 months before onset of clinical and laboratory signs of disease progression. The leukemia-specific hypermethylation of CpG sequences of the 5'-region of the calcitonin gene is a promising prognostic and diagnostic marker of leukemias and might be useful for monitoring of this disease.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1023/B:BIRY.0000022067.63532.b8</identifier><identifier>PMID: 15061703</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>5' Flanking Region - genetics ; Biomarkers, Tumor - genetics ; Bone marrow ; Calcitonin - genetics ; CpG Islands - genetics ; DNA Methylation ; Female ; Humans ; Leukemia ; Leukemia - diagnosis ; Leukemia - genetics ; Leukemia - therapy ; Male ; Medical research ; Medical treatment ; Methylation ; Neoplasm, Residual - genetics ; Predictive Value of Tests ; Recurrence ; Remission Induction</subject><ispartof>Biochemistry (Moscow), 2004-03, Vol.69 (3), p.340-349</ispartof><rights>MAIK "Nauka/Interperiodica" 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-6a90b1dcd49cb3b800409cdb56206a35ca90bda5a82801a2a75bb92e9b4dfc373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15061703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marinitch, D V</creatorcontrib><creatorcontrib>Vorobyev, I A</creatorcontrib><creatorcontrib>Holmes, J A</creatorcontrib><creatorcontrib>Zakharchenko, N S</creatorcontrib><creatorcontrib>Dyachenko, O V</creatorcontrib><creatorcontrib>Buryanov, Ya I</creatorcontrib><creatorcontrib>Shevchuk, T V</creatorcontrib><title>Hypermethylation of 5'-region of the human calcitonin gene in leukemias: structural features and diagnostic significance</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry (Mosc)</addtitle><description>Methylation of the 5'-region of the calcitonin gene was investigated in bone marrow and peripheral blood cells of 27 healthy volunteers and 25 leukemic patients. In all patients suffering from various forms of myeloid and lymphoid leukemia, hypermethylation of CpG sequences was observed in this region of the calcitonin gene. Cytosine hypermethylation in the CpG sequence did not involve cytosines of adjacent CpNpG sequences (where N is any nucleoside). The 5'-region of the calcitonin gene lacked CpNpG methylation both in healthy controls and in leukemic patients; this apparently represents specific "non-alternative" type of CpG methylation in the extended DNA sequence. Methylation of the calcitonin gene was monitored in 18 leukemic patients during malignant progression and medical treatment. Hypermethylation of the calcitonin gene was not observed on long-term clinical hematological remission. In ten patients characterized by unstable (or incomplete) remission hypermethylation of the calcitonin gene persisted through the whole period of observation. In relapses, hypermethylation of the calcitonin gene appeared again and in six patients, this "molecular relapse" being registered 1-8 months before onset of clinical and laboratory signs of disease progression. The leukemia-specific hypermethylation of CpG sequences of the 5'-region of the calcitonin gene is a promising prognostic and diagnostic marker of leukemias and might be useful for monitoring of this disease.</description><subject>5' Flanking Region - genetics</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Bone marrow</subject><subject>Calcitonin - genetics</subject><subject>CpG Islands - genetics</subject><subject>DNA Methylation</subject><subject>Female</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia - diagnosis</subject><subject>Leukemia - genetics</subject><subject>Leukemia - therapy</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical treatment</subject><subject>Methylation</subject><subject>Neoplasm, Residual - genetics</subject><subject>Predictive Value of Tests</subject><subject>Recurrence</subject><subject>Remission Induction</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1r3DAQhkVpaTZp_0IROTQnb_VhydbeuqFpAoFCSA89iZEs7yq15a0kQ_ffV24WAj1Ul5GYZ0a8PAhdUrKmhPFP28327uHHmiyHMSKbteSCs7VpX6EVlaStOKnJa7QqfVkx1agzdJ7S04ITxd-iMyqIpA3hK_T79nhwcXR5fxwg-yngqcfiqopud3rkvcP7eYSALQzW5yn4gHcuOFzq4OafbvSQNjjlONs8Rxhw76BcXMIQOtx52IUpZW9x8rvge28hWPcOvelhSO79qV6g7zdfHq9vq_tvX--uP99Xljd1riQoYmhnu1pZw01LSjJlOyNkCQ5c2KXfgYCWtYQCg0YYo5hTpu76soJfoKvnvYc4_Zpdynr0ybphgOCmOelG1ELWTKlCfvw_SRslCSUFvPwHfJrmGEqKwrSCtnWzQJtnyMYppeh6fYh-hHjUlOhFo97qRaN-0aj_atSmLcMfTj_MZnTdy-jJG_8DRJya_A</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Marinitch, D V</creator><creator>Vorobyev, I A</creator><creator>Holmes, J A</creator><creator>Zakharchenko, N S</creator><creator>Dyachenko, O V</creator><creator>Buryanov, Ya I</creator><creator>Shevchuk, T V</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7T5</scope></search><sort><creationdate>20040301</creationdate><title>Hypermethylation of 5'-region of the human calcitonin gene in leukemias: structural features and diagnostic significance</title><author>Marinitch, D V ; Vorobyev, I A ; Holmes, J A ; Zakharchenko, N S ; Dyachenko, O V ; Buryanov, Ya I ; Shevchuk, T V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-6a90b1dcd49cb3b800409cdb56206a35ca90bda5a82801a2a75bb92e9b4dfc373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>5' Flanking Region - genetics</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Bone marrow</topic><topic>Calcitonin - genetics</topic><topic>CpG Islands - genetics</topic><topic>DNA Methylation</topic><topic>Female</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia - diagnosis</topic><topic>Leukemia - genetics</topic><topic>Leukemia - therapy</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical treatment</topic><topic>Methylation</topic><topic>Neoplasm, Residual - genetics</topic><topic>Predictive Value of Tests</topic><topic>Recurrence</topic><topic>Remission Induction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marinitch, D V</creatorcontrib><creatorcontrib>Vorobyev, I A</creatorcontrib><creatorcontrib>Holmes, J A</creatorcontrib><creatorcontrib>Zakharchenko, N S</creatorcontrib><creatorcontrib>Dyachenko, O V</creatorcontrib><creatorcontrib>Buryanov, Ya I</creatorcontrib><creatorcontrib>Shevchuk, T V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><jtitle>Biochemistry (Moscow)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marinitch, D V</au><au>Vorobyev, I A</au><au>Holmes, J A</au><au>Zakharchenko, N S</au><au>Dyachenko, O V</au><au>Buryanov, Ya I</au><au>Shevchuk, T V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypermethylation of 5'-region of the human calcitonin gene in leukemias: structural features and diagnostic significance</atitle><jtitle>Biochemistry (Moscow)</jtitle><addtitle>Biochemistry (Mosc)</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>69</volume><issue>3</issue><spage>340</spage><epage>349</epage><pages>340-349</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>Methylation of the 5'-region of the calcitonin gene was investigated in bone marrow and peripheral blood cells of 27 healthy volunteers and 25 leukemic patients. In all patients suffering from various forms of myeloid and lymphoid leukemia, hypermethylation of CpG sequences was observed in this region of the calcitonin gene. Cytosine hypermethylation in the CpG sequence did not involve cytosines of adjacent CpNpG sequences (where N is any nucleoside). The 5'-region of the calcitonin gene lacked CpNpG methylation both in healthy controls and in leukemic patients; this apparently represents specific "non-alternative" type of CpG methylation in the extended DNA sequence. Methylation of the calcitonin gene was monitored in 18 leukemic patients during malignant progression and medical treatment. Hypermethylation of the calcitonin gene was not observed on long-term clinical hematological remission. In ten patients characterized by unstable (or incomplete) remission hypermethylation of the calcitonin gene persisted through the whole period of observation. In relapses, hypermethylation of the calcitonin gene appeared again and in six patients, this "molecular relapse" being registered 1-8 months before onset of clinical and laboratory signs of disease progression. The leukemia-specific hypermethylation of CpG sequences of the 5'-region of the calcitonin gene is a promising prognostic and diagnostic marker of leukemias and might be useful for monitoring of this disease.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>15061703</pmid><doi>10.1023/B:BIRY.0000022067.63532.b8</doi><tpages>10</tpages></addata></record> |
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| subjects | 5' Flanking Region - genetics Biomarkers, Tumor - genetics Bone marrow Calcitonin - genetics CpG Islands - genetics DNA Methylation Female Humans Leukemia Leukemia - diagnosis Leukemia - genetics Leukemia - therapy Male Medical research Medical treatment Methylation Neoplasm, Residual - genetics Predictive Value of Tests Recurrence Remission Induction |
| title | Hypermethylation of 5'-region of the human calcitonin gene in leukemias: structural features and diagnostic significance |
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