Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain
Abstract Modified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN® ) have been analyzed and shown to be 100% genetically identical...
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| Veröffentlicht in: | Vaccine 2009-12, Vol.27 (52), p.7442-7450 |
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| container_title | Vaccine |
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| creator | Suter, Mark Meisinger-Henschel, Christine Tzatzaris, Maria Hülsemann, Vanessa Lukassen, Susanne Wulff, Niels Holger Hausmann, Jürgen Howley, Paul Chaplin, Paul |
| description | Abstract Modified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN® ) have been analyzed and shown to be 100% genetically identical; although significant differences in their phenotypes were illustrated. All MVA strains except MVA-BN® replicated in human cells, or killed immune suppressed mice. Viruses isolated from dead animals were shown to represent variants present within MVA-572 or MVA-I721 used to inoculate the mice. These subpopulations were shown to encode mutations, or contain less than the six deletions associated with MVA and had significantly altered phenotypes compared to the parental MVA strains. MVA is a complex polyclonal mixture of viruses, the composition of which governs the phenotype. |
| doi_str_mv | 10.1016/j.vaccine.2009.05.095 |
| format | Article |
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After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN® ) have been analyzed and shown to be 100% genetically identical; although significant differences in their phenotypes were illustrated. All MVA strains except MVA-BN® replicated in human cells, or killed immune suppressed mice. Viruses isolated from dead animals were shown to represent variants present within MVA-572 or MVA-I721 used to inoculate the mice. These subpopulations were shown to encode mutations, or contain less than the six deletions associated with MVA and had significantly altered phenotypes compared to the parental MVA strains. MVA is a complex polyclonal mixture of viruses, the composition of which governs the phenotype.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2009.05.095</identifier><identifier>PMID: 19539582</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; Applied microbiology ; Biological and medical sciences ; Biological heterogeneity ; Biological properties ; Chick Embryo ; Deoxyribonucleic acid ; DNA ; DNA, Viral - analysis ; Embryos ; Female ; Fundamental and applied biological sciences. Psychology ; Genome, Viral ; Genomes ; HeLa Cells ; Humans ; Mammals ; Mice ; Mice, Knockout ; Microbiology ; MVA ; Ovary - virology ; Phenotype ; Sequence identity ; Serial Passage ; Studies ; Subpopulations ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccinia virus - genetics ; Vaccinia virus - physiology ; Virus Cultivation ; Virus Replication ; Viruses</subject><ispartof>Vaccine, 2009-12, Vol.27 (52), p.7442-7450</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Limited Dec 9, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-47db2019d802491587cde704a378f3babb4941ecbdbdfc32c678407a517f4cac3</citedby><cites>FETCH-LOGICAL-c509t-47db2019d802491587cde704a378f3babb4941ecbdbdfc32c678407a517f4cac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X0900838X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22289382$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19539582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suter, Mark</creatorcontrib><creatorcontrib>Meisinger-Henschel, Christine</creatorcontrib><creatorcontrib>Tzatzaris, Maria</creatorcontrib><creatorcontrib>Hülsemann, Vanessa</creatorcontrib><creatorcontrib>Lukassen, Susanne</creatorcontrib><creatorcontrib>Wulff, Niels Holger</creatorcontrib><creatorcontrib>Hausmann, Jürgen</creatorcontrib><creatorcontrib>Howley, Paul</creatorcontrib><creatorcontrib>Chaplin, Paul</creatorcontrib><title>Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Modified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN® ) have been analyzed and shown to be 100% genetically identical; although significant differences in their phenotypes were illustrated. All MVA strains except MVA-BN® replicated in human cells, or killed immune suppressed mice. Viruses isolated from dead animals were shown to represent variants present within MVA-572 or MVA-I721 used to inoculate the mice. These subpopulations were shown to encode mutations, or contain less than the six deletions associated with MVA and had significantly altered phenotypes compared to the parental MVA strains. MVA is a complex polyclonal mixture of viruses, the composition of which governs the phenotype.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Biological heterogeneity</subject><subject>Biological properties</subject><subject>Chick Embryo</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>Embryos</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Mammals</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Microbiology</subject><subject>MVA</subject><subject>Ovary - virology</subject><subject>Phenotype</subject><subject>Sequence identity</subject><subject>Serial Passage</subject><subject>Studies</subject><subject>Subpopulations</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccinia virus - genetics</subject><subject>Vaccinia virus - physiology</subject><subject>Virus Cultivation</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkstuEzEUhkcIREPhEUCWEGKV4OvY3oCqiptUxAKQurM89pnG6VyC7QnNE_GaOM2ISt105Yu-8_s__k9VvSR4RTCp321WO-tcGGBFMdYrLFZYi0fVgijJllQQ9bhaYFrzJSf48qR6ltIGYywY0U-rE6IF00LRRfX32-hDG8Cjo1yw6Gy4ttGilKMNQ0J_Ql6j4GHIwdkOucIPVyjB7wkGBwlZlyfbdXsUYRshFa4w_baDG9SHmzyVOzS2aBfilMo2r21GHjLEvpgvR0BNGLvx6lZ9G8ctxByONWDdevbxvHrS2i7Bi3k9rX59-vjz_Mvy4vvnr-dnF0snsM5LLn1DMdFeYco1EUo6DxJzy6RqWWObhmtOwDW-8a1j1NVScSytILLlzjp2Wr096hYnpcOUTR-Sg66zA4xTMlJwUTNF-cMk46RmUshCvr5HbsYpDqUNQ2qiNCVY00KJI-XimFKE1mxj6G3cG4LNIXKzMXPk5hC5wcKUyEvdq1l9anrwd1VzxgV4MwM2lT9uox1cSP85SqnS7Jb7cOSg_O8uQDTJhUPGPkRw2fgxPGjl_T0F15WRKo9ewx7SXdcmUYPNj8N8HsYTa4wVU5fsH3P15ZQ</recordid><startdate>20091209</startdate><enddate>20091209</enddate><creator>Suter, Mark</creator><creator>Meisinger-Henschel, Christine</creator><creator>Tzatzaris, Maria</creator><creator>Hülsemann, Vanessa</creator><creator>Lukassen, Susanne</creator><creator>Wulff, Niels Holger</creator><creator>Hausmann, Jürgen</creator><creator>Howley, Paul</creator><creator>Chaplin, Paul</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20091209</creationdate><title>Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain</title><author>Suter, Mark ; Meisinger-Henschel, Christine ; Tzatzaris, Maria ; Hülsemann, Vanessa ; Lukassen, Susanne ; Wulff, Niels Holger ; Hausmann, Jürgen ; Howley, Paul ; Chaplin, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-47db2019d802491587cde704a378f3babb4941ecbdbdfc32c678407a517f4cac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Biological heterogeneity</topic><topic>Biological properties</topic><topic>Chick Embryo</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - analysis</topic><topic>Embryos</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Viral</topic><topic>Genomes</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Mammals</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Microbiology</topic><topic>MVA</topic><topic>Ovary - virology</topic><topic>Phenotype</topic><topic>Sequence identity</topic><topic>Serial Passage</topic><topic>Studies</topic><topic>Subpopulations</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccinia virus - genetics</topic><topic>Vaccinia virus - physiology</topic><topic>Virus Cultivation</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suter, Mark</creatorcontrib><creatorcontrib>Meisinger-Henschel, Christine</creatorcontrib><creatorcontrib>Tzatzaris, Maria</creatorcontrib><creatorcontrib>Hülsemann, Vanessa</creatorcontrib><creatorcontrib>Lukassen, Susanne</creatorcontrib><creatorcontrib>Wulff, Niels Holger</creatorcontrib><creatorcontrib>Hausmann, Jürgen</creatorcontrib><creatorcontrib>Howley, Paul</creatorcontrib><creatorcontrib>Chaplin, Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Biological Sciences</collection><collection>ProQuest Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Healthcare Administration Database</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suter, Mark</au><au>Meisinger-Henschel, Christine</au><au>Tzatzaris, Maria</au><au>Hülsemann, Vanessa</au><au>Lukassen, Susanne</au><au>Wulff, Niels Holger</au><au>Hausmann, Jürgen</au><au>Howley, Paul</au><au>Chaplin, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2009-12-09</date><risdate>2009</risdate><volume>27</volume><issue>52</issue><spage>7442</spage><epage>7450</epage><pages>7442-7450</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Modified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN® ) have been analyzed and shown to be 100% genetically identical; although significant differences in their phenotypes were illustrated. All MVA strains except MVA-BN® replicated in human cells, or killed immune suppressed mice. Viruses isolated from dead animals were shown to represent variants present within MVA-572 or MVA-I721 used to inoculate the mice. These subpopulations were shown to encode mutations, or contain less than the six deletions associated with MVA and had significantly altered phenotypes compared to the parental MVA strains. MVA is a complex polyclonal mixture of viruses, the composition of which governs the phenotype.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19539582</pmid><doi>10.1016/j.vaccine.2009.05.095</doi><tpages>9</tpages></addata></record> |
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| subjects | Allergy and Immunology Animals Applied microbiology Biological and medical sciences Biological heterogeneity Biological properties Chick Embryo Deoxyribonucleic acid DNA DNA, Viral - analysis Embryos Female Fundamental and applied biological sciences. Psychology Genome, Viral Genomes HeLa Cells Humans Mammals Mice Mice, Knockout Microbiology MVA Ovary - virology Phenotype Sequence identity Serial Passage Studies Subpopulations Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccinia virus - genetics Vaccinia virus - physiology Virus Cultivation Virus Replication Viruses |
| title | Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain |
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