Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material
The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance a...
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| Veröffentlicht in: | Bioresource technology 2010-11, Vol.101 (21), p.8446-8451 |
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| creator | Nogueira, Grínia M. Rodas, Andrea C.D. Leite, Carlos A.P. Giles, Carlos Higa, Olga Z. Polakiewicz, Bronislaw Beppu, Marisa M. |
| description | The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance and cytotoxicity of membranes as well as the changes on the secondary structure of silk fibroin were analyzed after undergoing treatment with ethanol. Membranes presented amorphous patterns as determined via X-ray diffraction. The secondary structure of silk fibroin on dense membranes was either random coil (silk I) or β-sheet (silk II), before and after ethanol treatment, respectively. The sterilized membranes presented no cytotoxicity to endothelial cells during
in vitro assays. This fact stresses the material potential to be used in the fabrication of biomaterials, as coatings of cardiovascular devices and as membranes for wound dressing or drug delivery systems. |
| doi_str_mv | 10.1016/j.biortech.2010.06.064 |
| format | Article |
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in vitro assays. This fact stresses the material potential to be used in the fabrication of biomaterials, as coatings of cardiovascular devices and as membranes for wound dressing or drug delivery systems.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biocompatible Materials - chemical synthesis</subject><subject>Biological and medical sciences</subject><subject>Biomaterials</subject><subject>Biotechnology - methods</subject><subject>Calorimetry, Differential Scanning</subject><subject>Cell Proliferation - drug effects</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Dense membranes</subject><subject>Devices</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Ethanol</subject><subject>Ethanol - pharmacology</subject><subject>Ethyl alcohol</subject><subject>Exact sciences and technology</subject><subject>Fibroins - chemistry</subject><subject>Fibroins - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Mechanical Phenomena - drug effects</subject><subject>Membranes</subject><subject>Membranes, Artificial</subject><subject>Pollution</subject><subject>Silk</subject><subject>Silk fibroin</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surgical implants</subject><subject>Temperature</subject><subject>Tensile Strength - drug effects</subject><subject>Thermogravimetry</subject><subject>Waste Products - analysis</subject><subject>Wastes</subject><subject>X-Ray Diffraction</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoMo7rj6F9bciN50PGnaJL1zWfyCBQXd65AmpzsZ22ZMMi76P_y_ZujMeqdwIOTwvO_5IuSCwZoBE6-3696HmNFu1jWUJIgSzQOyYkryqu6keEhW0AmoVFs3Z-RJSlsA4EzWj8lZDW2nlJQr8vtzxJ2JJvswUzM7ajflZzNG_2tJhoFi3pg5jFWOaDI6mvz4jQ6-j8HP1OGckE449dHMmOgQIi29TebgYcZEzW43eruY7ZOfb-mdSRnvXTAWJtFo7uhJ9JQ8GooUnx3fc3Lz7u3Xqw_V9af3H68urysrAHJlayl40zBpuXHKCNH1zjVKKhADWFNzbITplO27vmFCYIfcOaUG26Oy0Lf8nLxcfHcxfN9jynryyeI4lknCPmnZNq3gEupCvvonyaSU0JWlsoKKBbUxpBRx0LvoJxN_agb6cDy91afj6cPxNIgSTRFeHGvs-wndvex0rQK8OAImWTMOZeHWp78ch5aVfRTu-cINJmhzGwtz86VU4sCUkMAP47xZCCzb_eEx6mQ9zhadj2izdsH_r9s_jGvIaA</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Nogueira, Grínia M.</creator><creator>Rodas, Andrea C.D.</creator><creator>Leite, Carlos A.P.</creator><creator>Giles, Carlos</creator><creator>Higa, Olga Z.</creator><creator>Polakiewicz, Bronislaw</creator><creator>Beppu, Marisa M.</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>7QO</scope><scope>7ST</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20101101</creationdate><title>Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material</title><author>Nogueira, Grínia M. ; Rodas, Andrea C.D. ; Leite, Carlos A.P. ; Giles, Carlos ; Higa, Olga Z. ; Polakiewicz, Bronislaw ; Beppu, Marisa M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-c27634417c3ad8a669bdd487806f0ca23e46a98cb9b4166e9e3dd88fcbe8c0b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biocompatible Materials - chemical synthesis</topic><topic>Biological and medical sciences</topic><topic>Biomaterials</topic><topic>Biotechnology - methods</topic><topic>Calorimetry, Differential Scanning</topic><topic>Cell Proliferation - drug effects</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Dense membranes</topic><topic>Devices</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Ethanol</topic><topic>Ethanol - pharmacology</topic><topic>Ethyl alcohol</topic><topic>Exact sciences and technology</topic><topic>Fibroins - chemistry</topic><topic>Fibroins - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mechanical Phenomena - drug effects</topic><topic>Membranes</topic><topic>Membranes, Artificial</topic><topic>Pollution</topic><topic>Silk</topic><topic>Silk fibroin</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Surgical implants</topic><topic>Temperature</topic><topic>Tensile Strength - drug effects</topic><topic>Thermogravimetry</topic><topic>Waste Products - analysis</topic><topic>Wastes</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nogueira, Grínia M.</creatorcontrib><creatorcontrib>Rodas, Andrea C.D.</creatorcontrib><creatorcontrib>Leite, Carlos A.P.</creatorcontrib><creatorcontrib>Giles, Carlos</creatorcontrib><creatorcontrib>Higa, Olga Z.</creatorcontrib><creatorcontrib>Polakiewicz, Bronislaw</creatorcontrib><creatorcontrib>Beppu, Marisa M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nogueira, Grínia M.</au><au>Rodas, Andrea C.D.</au><au>Leite, Carlos A.P.</au><au>Giles, Carlos</au><au>Higa, Olga Z.</au><au>Polakiewicz, Bronislaw</au><au>Beppu, Marisa M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>101</volume><issue>21</issue><spage>8446</spage><epage>8451</epage><pages>8446-8451</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance and cytotoxicity of membranes as well as the changes on the secondary structure of silk fibroin were analyzed after undergoing treatment with ethanol. Membranes presented amorphous patterns as determined via X-ray diffraction. The secondary structure of silk fibroin on dense membranes was either random coil (silk I) or β-sheet (silk II), before and after ethanol treatment, respectively. The sterilized membranes presented no cytotoxicity to endothelial cells during
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| ispartof | Bioresource technology, 2010-11, Vol.101 (21), p.8446-8451 |
| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Applied sciences Biocompatible Materials - chemical synthesis Biological and medical sciences Biomaterials Biotechnology - methods Calorimetry, Differential Scanning Cell Proliferation - drug effects CHO Cells Cricetinae Cricetulus Dense membranes Devices Endothelial Cells - cytology Endothelial Cells - drug effects Ethanol Ethanol - pharmacology Ethyl alcohol Exact sciences and technology Fibroins - chemistry Fibroins - ultrastructure Fundamental and applied biological sciences. Psychology Humans Mechanical Phenomena - drug effects Membranes Membranes, Artificial Pollution Silk Silk fibroin Spectroscopy, Fourier Transform Infrared Surgical implants Temperature Tensile Strength - drug effects Thermogravimetry Waste Products - analysis Wastes X-Ray Diffraction |
| title | Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material |
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