Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment
Background and Objectives We studied antitumor effects and cell death induced by cationic liposome‐mediated gene transfer of the herpes simplex virus thymidine kinase (HSV‐tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell l...
Gespeichert in:
| Veröffentlicht in: | Journal of surgical oncology 2001-01, Vol.76 (1), p.19-25 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 25 |
|---|---|
| container_issue | 1 |
| container_start_page | 19 |
| container_title | Journal of surgical oncology |
| container_volume | 76 |
| creator | Yoshida, Tazuka Mizuno, Masaaki Taniguchi, Katsumi Nakayashiki, Norihisa Wakabayashi, Toshihiko Yoshida, Jun |
| description | Background and Objectives
We studied antitumor effects and cell death induced by cationic liposome‐mediated gene transfer of the herpes simplex virus thymidine kinase (HSV‐tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell line.
Methods
To transfer genes we used small unilamellar cationic liposomes containing N‐(α‐trimethylammonioacetyl)‐didodecyl‐D‐glutamate chloride. Video‐enhanced contrast differential interference contrast microscopy was used for morphologic observations of cultured cells.
Results
When we treated the cells or implanted gliomas with the liposomes and ganciclovir, a strong effect was seen against tumor cells, and survival of tumor‐implanted rats was increased. Morphologically, cell death observed after HSV‐tk gene/liposome and ganciclovir treatment in the cultured glioma cells included both apoptosis and necrosis.
Conclusions
Introduction of the HSV‐tk gene in a DNA‐liposome complex followed by ganciclovir treatment induced both apoptosis and necrosis, which together resulted in a potent antitumor effect. J. Surg. Oncol. 2001;76:19–25. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1096-9098(200101)76:1<19::AID-JSO1004>3.0.CO;2-D |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754563301</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>754563301</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5284-ff4f7f3cecf90c14d07889901cbfb58eb154d01a8dc2db4ef24cb68c5239d9d33</originalsourceid><addsrcrecordid>eNqNkdtuEzEQhlcIREvhFZAlJA4XG3zYgx0QUpXQ0ioiCIqQuBl5vePEdA9hvaHNm_C4eLUh3CDEla2Zb_75NX8USUYnjFL-klGVxYoq-ZxTyih7kWdT9pqp6fT0Yh5ffloGKnkjJnQyW77i8fxOdHwYuRsdBwkeJ7miR9ED779RSpXKkvvREWOcC8npcfTzo-7JqnJtrYnBqiIl6n5NXFNuDZak2BGje9c2zpDKbVrf1hjXWDrdh27f6cZb7EhrSb9GssZug554V28qvCU_XLf1obGrXekaJNeu0R7JCsPftlXV3owbVroxzlRt4INk2F9j0z-M7lldeXy0f0-iz2dvr2bv4sXy_GJ2uohNymUSW5vY3AqDxipqWFLSXEqlKDOFLVKJBUtDjWlZGl4WCVqemCKTYVioUpVCnETPRt1N137fou-hdn64hG6w3XrI0yTNhKAskE__TdIs5cFUAD-MoOla7zu0sOlcrbsdMApDrjCEBENIMOYKeQYMmAIIucI-VxBAYbYEDvMg-Xi_e1uE8_8R3AcZgCd7QHujK9sNN_UHTua5FIOzq5G6cRXu_t_W3139LgXZeJR1vsfbg6zuriHLRZ7Cl_fn8PVSLmSeUTgTvwALktp3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70652528</pqid></control><display><type>article</type><title>Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Yoshida, Tazuka ; Mizuno, Masaaki ; Taniguchi, Katsumi ; Nakayashiki, Norihisa ; Wakabayashi, Toshihiko ; Yoshida, Jun</creator><creatorcontrib>Yoshida, Tazuka ; Mizuno, Masaaki ; Taniguchi, Katsumi ; Nakayashiki, Norihisa ; Wakabayashi, Toshihiko ; Yoshida, Jun</creatorcontrib><description>Background and Objectives
We studied antitumor effects and cell death induced by cationic liposome‐mediated gene transfer of the herpes simplex virus thymidine kinase (HSV‐tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell line.
Methods
To transfer genes we used small unilamellar cationic liposomes containing N‐(α‐trimethylammonioacetyl)‐didodecyl‐D‐glutamate chloride. Video‐enhanced contrast differential interference contrast microscopy was used for morphologic observations of cultured cells.
Results
When we treated the cells or implanted gliomas with the liposomes and ganciclovir, a strong effect was seen against tumor cells, and survival of tumor‐implanted rats was increased. Morphologically, cell death observed after HSV‐tk gene/liposome and ganciclovir treatment in the cultured glioma cells included both apoptosis and necrosis.
Conclusions
Introduction of the HSV‐tk gene in a DNA‐liposome complex followed by ganciclovir treatment induced both apoptosis and necrosis, which together resulted in a potent antitumor effect. J. Surg. Oncol. 2001;76:19–25. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/1096-9098(200101)76:1<19::AID-JSO1004>3.0.CO;2-D</identifier><identifier>PMID: 11223820</identifier><identifier>CODEN: JSONAU</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Antiviral Agents - pharmacology ; Apoptosis ; Biological and medical sciences ; Brain Neoplasms - genetics ; Brain Neoplasms - pathology ; Cations ; Cell Death ; cells ; culture ; Ganciclovir - pharmacology ; Gene Transfer Techniques ; Glioma - genetics ; Glioma - pathology ; Herpes simplex virus ; Liposomes ; Medical sciences ; Microscopy, Interference ; necrosis ; Neurology ; Other treatments ; Rats ; Simplexvirus - genetics ; Thymidine Kinase - genetics ; transplanted brain tumors ; Treatment. General aspects ; Tumor Cells, Cultured ; Tumors ; Tumors of the nervous system. Phacomatoses ; video-enhanced contrast differential interference contrast microscopy</subject><ispartof>Journal of surgical oncology, 2001-01, Vol.76 (1), p.19-25</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5284-ff4f7f3cecf90c14d07889901cbfb58eb154d01a8dc2db4ef24cb68c5239d9d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1096-9098%28200101%2976%3A1%3C19%3A%3AAID-JSO1004%3E3.0.CO%3B2-D$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1096-9098%28200101%2976%3A1%3C19%3A%3AAID-JSO1004%3E3.0.CO%3B2-D$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=877838$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11223820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshida, Tazuka</creatorcontrib><creatorcontrib>Mizuno, Masaaki</creatorcontrib><creatorcontrib>Taniguchi, Katsumi</creatorcontrib><creatorcontrib>Nakayashiki, Norihisa</creatorcontrib><creatorcontrib>Wakabayashi, Toshihiko</creatorcontrib><creatorcontrib>Yoshida, Jun</creatorcontrib><title>Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>Background and Objectives
We studied antitumor effects and cell death induced by cationic liposome‐mediated gene transfer of the herpes simplex virus thymidine kinase (HSV‐tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell line.
Methods
To transfer genes we used small unilamellar cationic liposomes containing N‐(α‐trimethylammonioacetyl)‐didodecyl‐D‐glutamate chloride. Video‐enhanced contrast differential interference contrast microscopy was used for morphologic observations of cultured cells.
Results
When we treated the cells or implanted gliomas with the liposomes and ganciclovir, a strong effect was seen against tumor cells, and survival of tumor‐implanted rats was increased. Morphologically, cell death observed after HSV‐tk gene/liposome and ganciclovir treatment in the cultured glioma cells included both apoptosis and necrosis.
Conclusions
Introduction of the HSV‐tk gene in a DNA‐liposome complex followed by ganciclovir treatment induced both apoptosis and necrosis, which together resulted in a potent antitumor effect. J. Surg. Oncol. 2001;76:19–25. © 2001 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Antiviral Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - pathology</subject><subject>Cations</subject><subject>Cell Death</subject><subject>cells</subject><subject>culture</subject><subject>Ganciclovir - pharmacology</subject><subject>Gene Transfer Techniques</subject><subject>Glioma - genetics</subject><subject>Glioma - pathology</subject><subject>Herpes simplex virus</subject><subject>Liposomes</subject><subject>Medical sciences</subject><subject>Microscopy, Interference</subject><subject>necrosis</subject><subject>Neurology</subject><subject>Other treatments</subject><subject>Rats</subject><subject>Simplexvirus - genetics</subject><subject>Thymidine Kinase - genetics</subject><subject>transplanted brain tumors</subject><subject>Treatment. General aspects</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Tumors of the nervous system. Phacomatoses</subject><subject>video-enhanced contrast differential interference contrast microscopy</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdtuEzEQhlcIREvhFZAlJA4XG3zYgx0QUpXQ0ioiCIqQuBl5vePEdA9hvaHNm_C4eLUh3CDEla2Zb_75NX8USUYnjFL-klGVxYoq-ZxTyih7kWdT9pqp6fT0Yh5ffloGKnkjJnQyW77i8fxOdHwYuRsdBwkeJ7miR9ED779RSpXKkvvREWOcC8npcfTzo-7JqnJtrYnBqiIl6n5NXFNuDZak2BGje9c2zpDKbVrf1hjXWDrdh27f6cZb7EhrSb9GssZug554V28qvCU_XLf1obGrXekaJNeu0R7JCsPftlXV3owbVroxzlRt4INk2F9j0z-M7lldeXy0f0-iz2dvr2bv4sXy_GJ2uohNymUSW5vY3AqDxipqWFLSXEqlKDOFLVKJBUtDjWlZGl4WCVqemCKTYVioUpVCnETPRt1N137fou-hdn64hG6w3XrI0yTNhKAskE__TdIs5cFUAD-MoOla7zu0sOlcrbsdMApDrjCEBENIMOYKeQYMmAIIucI-VxBAYbYEDvMg-Xi_e1uE8_8R3AcZgCd7QHujK9sNN_UHTua5FIOzq5G6cRXu_t_W3139LgXZeJR1vsfbg6zuriHLRZ7Cl_fn8PVSLmSeUTgTvwALktp3</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Yoshida, Tazuka</creator><creator>Mizuno, Masaaki</creator><creator>Taniguchi, Katsumi</creator><creator>Nakayashiki, Norihisa</creator><creator>Wakabayashi, Toshihiko</creator><creator>Yoshida, Jun</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>200101</creationdate><title>Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment</title><author>Yoshida, Tazuka ; Mizuno, Masaaki ; Taniguchi, Katsumi ; Nakayashiki, Norihisa ; Wakabayashi, Toshihiko ; Yoshida, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5284-ff4f7f3cecf90c14d07889901cbfb58eb154d01a8dc2db4ef24cb68c5239d9d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antiviral Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - pathology</topic><topic>Cations</topic><topic>Cell Death</topic><topic>cells</topic><topic>culture</topic><topic>Ganciclovir - pharmacology</topic><topic>Gene Transfer Techniques</topic><topic>Glioma - genetics</topic><topic>Glioma - pathology</topic><topic>Herpes simplex virus</topic><topic>Liposomes</topic><topic>Medical sciences</topic><topic>Microscopy, Interference</topic><topic>necrosis</topic><topic>Neurology</topic><topic>Other treatments</topic><topic>Rats</topic><topic>Simplexvirus - genetics</topic><topic>Thymidine Kinase - genetics</topic><topic>transplanted brain tumors</topic><topic>Treatment. General aspects</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Tumors of the nervous system. Phacomatoses</topic><topic>video-enhanced contrast differential interference contrast microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshida, Tazuka</creatorcontrib><creatorcontrib>Mizuno, Masaaki</creatorcontrib><creatorcontrib>Taniguchi, Katsumi</creatorcontrib><creatorcontrib>Nakayashiki, Norihisa</creatorcontrib><creatorcontrib>Wakabayashi, Toshihiko</creatorcontrib><creatorcontrib>Yoshida, Jun</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshida, Tazuka</au><au>Mizuno, Masaaki</au><au>Taniguchi, Katsumi</au><au>Nakayashiki, Norihisa</au><au>Wakabayashi, Toshihiko</au><au>Yoshida, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>2001-01</date><risdate>2001</risdate><volume>76</volume><issue>1</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><coden>JSONAU</coden><abstract>Background and Objectives
We studied antitumor effects and cell death induced by cationic liposome‐mediated gene transfer of the herpes simplex virus thymidine kinase (HSV‐tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell line.
Methods
To transfer genes we used small unilamellar cationic liposomes containing N‐(α‐trimethylammonioacetyl)‐didodecyl‐D‐glutamate chloride. Video‐enhanced contrast differential interference contrast microscopy was used for morphologic observations of cultured cells.
Results
When we treated the cells or implanted gliomas with the liposomes and ganciclovir, a strong effect was seen against tumor cells, and survival of tumor‐implanted rats was increased. Morphologically, cell death observed after HSV‐tk gene/liposome and ganciclovir treatment in the cultured glioma cells included both apoptosis and necrosis.
Conclusions
Introduction of the HSV‐tk gene in a DNA‐liposome complex followed by ganciclovir treatment induced both apoptosis and necrosis, which together resulted in a potent antitumor effect. J. Surg. Oncol. 2001;76:19–25. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11223820</pmid><doi>10.1002/1096-9098(200101)76:1<19::AID-JSO1004>3.0.CO;2-D</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4790 |
| ispartof | Journal of surgical oncology, 2001-01, Vol.76 (1), p.19-25 |
| issn | 0022-4790 1096-9098 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_754563301 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animals Antiviral Agents - pharmacology Apoptosis Biological and medical sciences Brain Neoplasms - genetics Brain Neoplasms - pathology Cations Cell Death cells culture Ganciclovir - pharmacology Gene Transfer Techniques Glioma - genetics Glioma - pathology Herpes simplex virus Liposomes Medical sciences Microscopy, Interference necrosis Neurology Other treatments Rats Simplexvirus - genetics Thymidine Kinase - genetics transplanted brain tumors Treatment. General aspects Tumor Cells, Cultured Tumors Tumors of the nervous system. Phacomatoses video-enhanced contrast differential interference contrast microscopy |
| title | Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T02%3A44%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rat%20glioma%20cell%20death%20induced%20by%20cationic%20liposome-mediated%20transfer%20of%20the%20herpes%20simplex%20virus%20thymidine%20kinase%20gene%20followed%20by%20ganciclovir%20treatment&rft.jtitle=Journal%20of%20surgical%20oncology&rft.au=Yoshida,%20Tazuka&rft.date=2001-01&rft.volume=76&rft.issue=1&rft.spage=19&rft.epage=25&rft.pages=19-25&rft.issn=0022-4790&rft.eissn=1096-9098&rft.coden=JSONAU&rft_id=info:doi/10.1002/1096-9098(200101)76:1%3C19::AID-JSO1004%3E3.0.CO;2-D&rft_dat=%3Cproquest_cross%3E754563301%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70652528&rft_id=info:pmid/11223820&rfr_iscdi=true |