Salt is necessary for nucleosomal DNA fragmentation induced by caspase
For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone c...
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| Veröffentlicht in: | Biotechnology letters 2005-12, Vol.27 (23-24), p.1935-1942 |
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| container_end_page | 1942 |
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| container_issue | 23-24 |
| container_start_page | 1935 |
| container_title | Biotechnology letters |
| container_volume | 27 |
| creator | KIM, Bo-Eun LEE, Yun-Hee SEONG, Changhyun SONG YUB SHIN JUNG SUP LEE BYUNG YUN CHO PARK, Il-Seon |
| description | For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s). |
| doi_str_mv | 10.1007/s10529-005-3906-z |
| format | Article |
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The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-005-3906-z</identifier><identifier>PMID: 16328993</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Apoptosis Regulatory Proteins ; Aurintricarboxylic Acid - pharmacology ; Biological and medical sciences ; Biotechnology ; Caspase 3 ; Caspase Inhibitors ; Caspases - genetics ; Caspases - metabolism ; Cell Nucleus - drug effects ; Cell Nucleus - genetics ; Cell Nucleus - metabolism ; Chromatin - drug effects ; Chromatin - metabolism ; Chromatography, Gel ; Computer aided design ; Cysteine ; Cysteine Proteinase Inhibitors - pharmacology ; Deoxyribonucleases - metabolism ; Deoxyribonucleic acid ; DNA ; DNA Fragmentation - drug effects ; Fragmentation ; Fundamental and applied biological sciences. Psychology ; Humans ; Jurkat Cells ; Mutation - genetics ; Nucleosomes - drug effects ; Nucleosomes - genetics ; Nucleosomes - metabolism ; Oligopeptides - pharmacology ; Poly-ADP-Ribose Binding Proteins ; Potassium Chloride - pharmacology ; Protease ; Proteins ; Proteins - genetics ; Proteins - metabolism ; Rats ; Salts - pharmacology ; Sodium chloride ; Sodium Chloride - pharmacology</subject><ispartof>Biotechnology letters, 2005-12, Vol.27 (23-24), p.1935-1942</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-ff83e49f8017ed91fcd16893fbb6100f69179c4a282462147f7553c388f8c5b13</citedby><cites>FETCH-LOGICAL-c484t-ff83e49f8017ed91fcd16893fbb6100f69179c4a282462147f7553c388f8c5b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17349011$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16328993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, Bo-Eun</creatorcontrib><creatorcontrib>LEE, Yun-Hee</creatorcontrib><creatorcontrib>SEONG, Changhyun</creatorcontrib><creatorcontrib>SONG YUB SHIN</creatorcontrib><creatorcontrib>JUNG SUP LEE</creatorcontrib><creatorcontrib>BYUNG YUN CHO</creatorcontrib><creatorcontrib>PARK, Il-Seon</creatorcontrib><title>Salt is necessary for nucleosomal DNA fragmentation induced by caspase</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis Regulatory Proteins</subject><subject>Aurintricarboxylic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Caspase 3</subject><subject>Caspase Inhibitors</subject><subject>Caspases - genetics</subject><subject>Caspases - metabolism</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Chromatin - drug effects</subject><subject>Chromatin - metabolism</subject><subject>Chromatography, Gel</subject><subject>Computer aided design</subject><subject>Cysteine</subject><subject>Cysteine Proteinase Inhibitors - pharmacology</subject><subject>Deoxyribonucleases - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Fragmentation - drug effects</subject><subject>Fragmentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Jurkat Cells</subject><subject>Mutation - genetics</subject><subject>Nucleosomes - drug effects</subject><subject>Nucleosomes - genetics</subject><subject>Nucleosomes - metabolism</subject><subject>Oligopeptides - pharmacology</subject><subject>Poly-ADP-Ribose Binding Proteins</subject><subject>Potassium Chloride - pharmacology</subject><subject>Protease</subject><subject>Proteins</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Salts - pharmacology</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0rtOHDEUBmALJYIN8AA0kRWJJM0QH99dIggJEoKCpLY8HjsaNJfNnJkCnh5vdiWkFFC5-Xx8-X9CToCdAWPmGwJT3FWMqUo4pqunPbICZUSljdHvyIqBhEpJxw_IB8QHxpgzzOyTA9CCW-fEilzdh26mLdIhxYQYpkeax4kOS-zSiGMfOnp5e07zFP70aZjD3I4DbYdmiamh9SONAdcB0xF5n0OH6Xi3HpLfV99_Xfysbu5-XF-c31RRWjlXOVuRpMuWgUmNgxwb0NaJXNe6vChrB8ZFGbjlUnOQJhulRBTWZhtVDeKQfNnOXU_j3yXh7PsWY-q6MKRxQW-UVJprw96WUkgltRZFfn5VamuVdka9CcFwXuwGfn0daqtsCeDfNT_9Rx_GZRrKH3ojpC1xWV4QbFGcRsQpZb-e2r5k5YH5TRP8tgm-NMFvmuCfyp6Pu8FL3afmZccu-gJOdyBgDF1JeIgtvrhyvGMA4hk00Lhn</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>KIM, Bo-Eun</creator><creator>LEE, Yun-Hee</creator><creator>SEONG, Changhyun</creator><creator>SONG YUB SHIN</creator><creator>JUNG SUP LEE</creator><creator>BYUNG YUN CHO</creator><creator>PARK, Il-Seon</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Salt is necessary for nucleosomal DNA fragmentation induced by caspase</title><author>KIM, Bo-Eun ; LEE, Yun-Hee ; SEONG, Changhyun ; SONG YUB SHIN ; JUNG SUP LEE ; BYUNG YUN CHO ; PARK, Il-Seon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-ff83e49f8017ed91fcd16893fbb6100f69179c4a282462147f7553c388f8c5b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis Regulatory Proteins</topic><topic>Aurintricarboxylic Acid - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Caspase 3</topic><topic>Caspase Inhibitors</topic><topic>Caspases - genetics</topic><topic>Caspases - metabolism</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Chromatin - drug effects</topic><topic>Chromatin - metabolism</topic><topic>Chromatography, Gel</topic><topic>Computer aided design</topic><topic>Cysteine</topic><topic>Cysteine Proteinase Inhibitors - pharmacology</topic><topic>Deoxyribonucleases - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Fragmentation - drug effects</topic><topic>Fragmentation</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIM, Bo-Eun</au><au>LEE, Yun-Hee</au><au>SEONG, Changhyun</au><au>SONG YUB SHIN</au><au>JUNG SUP LEE</au><au>BYUNG YUN CHO</au><au>PARK, Il-Seon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salt is necessary for nucleosomal DNA fragmentation induced by caspase</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>27</volume><issue>23-24</issue><spage>1935</spage><epage>1942</epage><pages>1935-1942</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>16328993</pmid><doi>10.1007/s10529-005-3906-z</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2005-12, Vol.27 (23-24), p.1935-1942 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| subjects | Animals Apoptosis Apoptosis - drug effects Apoptosis Regulatory Proteins Aurintricarboxylic Acid - pharmacology Biological and medical sciences Biotechnology Caspase 3 Caspase Inhibitors Caspases - genetics Caspases - metabolism Cell Nucleus - drug effects Cell Nucleus - genetics Cell Nucleus - metabolism Chromatin - drug effects Chromatin - metabolism Chromatography, Gel Computer aided design Cysteine Cysteine Proteinase Inhibitors - pharmacology Deoxyribonucleases - metabolism Deoxyribonucleic acid DNA DNA Fragmentation - drug effects Fragmentation Fundamental and applied biological sciences. Psychology Humans Jurkat Cells Mutation - genetics Nucleosomes - drug effects Nucleosomes - genetics Nucleosomes - metabolism Oligopeptides - pharmacology Poly-ADP-Ribose Binding Proteins Potassium Chloride - pharmacology Protease Proteins Proteins - genetics Proteins - metabolism Rats Salts - pharmacology Sodium chloride Sodium Chloride - pharmacology |
| title | Salt is necessary for nucleosomal DNA fragmentation induced by caspase |
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