Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy
Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigat...
Gespeichert in:
| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2010-08, Vol.104 (8), p.546-550 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 550 |
|---|---|
| container_issue | 8 |
| container_start_page | 546 |
| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| container_volume | 104 |
| creator | Da Silva, Rozana C. Lyon, Sandra Lyon, Ana C. Grossi, Maria A.F. Lyon, Silvia H. Bührer-Sékula, Samira Antunes, Carlos M.F. |
| description | Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigation involved 60 patients with newly diagnosed leprosy. Together with the application of the serological assays, selected demographic, clinical and epidemiological data relating to the study population were recorded. ML Flow detected anti-PGL1 antibodies in 70% of the leprosy patients, while ELISA was positive in 53.3%. The degree of concordance between the tests was substantial (83.3%). A positive correlation was demonstrated between the results obtained in the semi-quantitative ML Flow test and ELISA absorbance values. We concluded that both serological assays were found to be efficient in detecting anti-PGL1 antibodies. The ML Flow test may be a cheaper and easier to perform alternative to ELISA in leprosy patients. |
| doi_str_mv | 10.1016/j.trstmh.2010.05.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754562337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0035920310001100</els_id><sourcerecordid>754562337</sourcerecordid><originalsourceid>FETCH-LOGICAL-c461t-f48a43ca0b3d1176c8bade84bf3b6f88652d46e661bf0c0a3603bb85bba57be33</originalsourceid><addsrcrecordid>eNqF0c-P1CAUB3BiNO64-h8Yw8V46vgohTIXk93J_tJRY3ZNjBcC9DUydtoKjLvjXy-TjutNTyTweTx4X0KeM5gzYPL1ep5CTJtv8xLyFog5AHtAZkzVquAC-EMyA-CiWJTAj8iTGNcApWBi8ZgclSBLVjI1I2Y5hICdSX7oqcV0i9jTs9XV9Qk1fUPfr-h5N9xSE6PZRWrGsfPY0DRQCfQ0mF--86anY67HPmXQtuhSFnZHOxzDEHdPyaPWdBGfHdZj8vn87GZ5Waw-XlwtT1aFqyRLRVspU3FnwPKGsVo6ZU2DqrItt7JVSoqyqSRKyWwLDgyXwK1VwlojaoucH5NX0725648txqQ3PjrsOtPjsI26FpWQJef1_yXnC8VYtZfVJF3-SQzY6jH4jQk7zUDvU9BrPaWg9yloEDqnkMteHBps7Qab-6I_Y8_g5QGY6EzXBtM7H_86DgJUXWZXTM7HhHf35yZ817LmtdCXX77qt9cfPr3jNwsN2b-ZPOZB__QYdHQ5GIeNDzkX3Qz-3y__DTo2tjI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733981147</pqid></control><display><type>article</type><title>Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Da Silva, Rozana C. ; Lyon, Sandra ; Lyon, Ana C. ; Grossi, Maria A.F. ; Lyon, Silvia H. ; Bührer-Sékula, Samira ; Antunes, Carlos M.F.</creator><creatorcontrib>Da Silva, Rozana C. ; Lyon, Sandra ; Lyon, Ana C. ; Grossi, Maria A.F. ; Lyon, Silvia H. ; Bührer-Sékula, Samira ; Antunes, Carlos M.F.</creatorcontrib><description>Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigation involved 60 patients with newly diagnosed leprosy. Together with the application of the serological assays, selected demographic, clinical and epidemiological data relating to the study population were recorded. ML Flow detected anti-PGL1 antibodies in 70% of the leprosy patients, while ELISA was positive in 53.3%. The degree of concordance between the tests was substantial (83.3%). A positive correlation was demonstrated between the results obtained in the semi-quantitative ML Flow test and ELISA absorbance values. We concluded that both serological assays were found to be efficient in detecting anti-PGL1 antibodies. The ML Flow test may be a cheaper and easier to perform alternative to ELISA in leprosy patients.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/j.trstmh.2010.05.001</identifier><identifier>PMID: 20621218</identifier><identifier>CODEN: TRSTAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Absorbance ; Adolescent ; Antigens, Bacterial - immunology ; Bacterial diseases ; Biological and medical sciences ; Brazil - epidemiology ; Chromatography - methods ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; General aspects ; Glycolipids - immunology ; Human bacterial diseases ; Humans ; Immunoglobulin M - analysis ; Infectious diseases ; Leprosy ; Leprosy - diagnosis ; Leprosy - epidemiology ; Leprosy - immunology ; Male ; Medical sciences ; ML Flow ; Mycobacterium leprae ; Mycobacterium leprae - immunology ; PGL-1 antigen ; Serological assays ; Tropical bacterial diseases ; Tuberculosis and atypical mycobacterial infections ; Young Adult</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2010-08, Vol.104 (8), p.546-550</ispartof><rights>2010 Royal Society of Tropical Medicine and Hygiene</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-f48a43ca0b3d1176c8bade84bf3b6f88652d46e661bf0c0a3603bb85bba57be33</citedby><cites>FETCH-LOGICAL-c461t-f48a43ca0b3d1176c8bade84bf3b6f88652d46e661bf0c0a3603bb85bba57be33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23050872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20621218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Da Silva, Rozana C.</creatorcontrib><creatorcontrib>Lyon, Sandra</creatorcontrib><creatorcontrib>Lyon, Ana C.</creatorcontrib><creatorcontrib>Grossi, Maria A.F.</creatorcontrib><creatorcontrib>Lyon, Silvia H.</creatorcontrib><creatorcontrib>Bührer-Sékula, Samira</creatorcontrib><creatorcontrib>Antunes, Carlos M.F.</creatorcontrib><title>Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigation involved 60 patients with newly diagnosed leprosy. Together with the application of the serological assays, selected demographic, clinical and epidemiological data relating to the study population were recorded. ML Flow detected anti-PGL1 antibodies in 70% of the leprosy patients, while ELISA was positive in 53.3%. The degree of concordance between the tests was substantial (83.3%). A positive correlation was demonstrated between the results obtained in the semi-quantitative ML Flow test and ELISA absorbance values. We concluded that both serological assays were found to be efficient in detecting anti-PGL1 antibodies. The ML Flow test may be a cheaper and easier to perform alternative to ELISA in leprosy patients.</description><subject>Absorbance</subject><subject>Adolescent</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Brazil - epidemiology</subject><subject>Chromatography - methods</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>General aspects</subject><subject>Glycolipids - immunology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoglobulin M - analysis</subject><subject>Infectious diseases</subject><subject>Leprosy</subject><subject>Leprosy - diagnosis</subject><subject>Leprosy - epidemiology</subject><subject>Leprosy - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>ML Flow</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae - immunology</subject><subject>PGL-1 antigen</subject><subject>Serological assays</subject><subject>Tropical bacterial diseases</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Young Adult</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-P1CAUB3BiNO64-h8Yw8V46vgohTIXk93J_tJRY3ZNjBcC9DUydtoKjLvjXy-TjutNTyTweTx4X0KeM5gzYPL1ep5CTJtv8xLyFog5AHtAZkzVquAC-EMyA-CiWJTAj8iTGNcApWBi8ZgclSBLVjI1I2Y5hICdSX7oqcV0i9jTs9XV9Qk1fUPfr-h5N9xSE6PZRWrGsfPY0DRQCfQ0mF--86anY67HPmXQtuhSFnZHOxzDEHdPyaPWdBGfHdZj8vn87GZ5Waw-XlwtT1aFqyRLRVspU3FnwPKGsVo6ZU2DqrItt7JVSoqyqSRKyWwLDgyXwK1VwlojaoucH5NX0725648txqQ3PjrsOtPjsI26FpWQJef1_yXnC8VYtZfVJF3-SQzY6jH4jQk7zUDvU9BrPaWg9yloEDqnkMteHBps7Qab-6I_Y8_g5QGY6EzXBtM7H_86DgJUXWZXTM7HhHf35yZ817LmtdCXX77qt9cfPr3jNwsN2b-ZPOZB__QYdHQ5GIeNDzkX3Qz-3y__DTo2tjI</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Da Silva, Rozana C.</creator><creator>Lyon, Sandra</creator><creator>Lyon, Ana C.</creator><creator>Grossi, Maria A.F.</creator><creator>Lyon, Silvia H.</creator><creator>Bührer-Sékula, Samira</creator><creator>Antunes, Carlos M.F.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T2</scope><scope>7U2</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20100801</creationdate><title>Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy</title><author>Da Silva, Rozana C. ; Lyon, Sandra ; Lyon, Ana C. ; Grossi, Maria A.F. ; Lyon, Silvia H. ; Bührer-Sékula, Samira ; Antunes, Carlos M.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-f48a43ca0b3d1176c8bade84bf3b6f88652d46e661bf0c0a3603bb85bba57be33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Absorbance</topic><topic>Adolescent</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Brazil - epidemiology</topic><topic>Chromatography - methods</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>General aspects</topic><topic>Glycolipids - immunology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoglobulin M - analysis</topic><topic>Infectious diseases</topic><topic>Leprosy</topic><topic>Leprosy - diagnosis</topic><topic>Leprosy - epidemiology</topic><topic>Leprosy - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>ML Flow</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae - immunology</topic><topic>PGL-1 antigen</topic><topic>Serological assays</topic><topic>Tropical bacterial diseases</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Da Silva, Rozana C.</creatorcontrib><creatorcontrib>Lyon, Sandra</creatorcontrib><creatorcontrib>Lyon, Ana C.</creatorcontrib><creatorcontrib>Grossi, Maria A.F.</creatorcontrib><creatorcontrib>Lyon, Silvia H.</creatorcontrib><creatorcontrib>Bührer-Sékula, Samira</creatorcontrib><creatorcontrib>Antunes, Carlos M.F.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Da Silva, Rozana C.</au><au>Lyon, Sandra</au><au>Lyon, Ana C.</au><au>Grossi, Maria A.F.</au><au>Lyon, Silvia H.</au><au>Bührer-Sékula, Samira</au><au>Antunes, Carlos M.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>104</volume><issue>8</issue><spage>546</spage><epage>550</epage><pages>546-550</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>Serological tests can be helpful in classifying leprosy patients as having either the paucibacillary or the multibacillary form. The aim of this study was to evaluate the concordance between two serological assays, i.e. ML Flow and ELISA, in a population of leprosy patients in Brazil. The investigation involved 60 patients with newly diagnosed leprosy. Together with the application of the serological assays, selected demographic, clinical and epidemiological data relating to the study population were recorded. ML Flow detected anti-PGL1 antibodies in 70% of the leprosy patients, while ELISA was positive in 53.3%. The degree of concordance between the tests was substantial (83.3%). A positive correlation was demonstrated between the results obtained in the semi-quantitative ML Flow test and ELISA absorbance values. We concluded that both serological assays were found to be efficient in detecting anti-PGL1 antibodies. The ML Flow test may be a cheaper and easier to perform alternative to ELISA in leprosy patients.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>20621218</pmid><doi>10.1016/j.trstmh.2010.05.001</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 2010-08, Vol.104 (8), p.546-550 |
| issn | 0035-9203 1878-3503 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_754562337 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Absorbance Adolescent Antigens, Bacterial - immunology Bacterial diseases Biological and medical sciences Brazil - epidemiology Chromatography - methods ELISA Enzyme-Linked Immunosorbent Assay - methods Female General aspects Glycolipids - immunology Human bacterial diseases Humans Immunoglobulin M - analysis Infectious diseases Leprosy Leprosy - diagnosis Leprosy - epidemiology Leprosy - immunology Male Medical sciences ML Flow Mycobacterium leprae Mycobacterium leprae - immunology PGL-1 antigen Serological assays Tropical bacterial diseases Tuberculosis and atypical mycobacterial infections Young Adult |
| title | Correlation between ELISA and ML Flow assays applied to 60 Brazilian patients affected by leprosy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T04%3A11%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Correlation%20between%20ELISA%20and%20ML%20Flow%20assays%20applied%20to%2060%20Brazilian%20patients%20affected%20by%20leprosy&rft.jtitle=Transactions%20of%20the%20Royal%20Society%20of%20Tropical%20Medicine%20and%20Hygiene&rft.au=Da%20Silva,%20Rozana%20C.&rft.date=2010-08-01&rft.volume=104&rft.issue=8&rft.spage=546&rft.epage=550&rft.pages=546-550&rft.issn=0035-9203&rft.eissn=1878-3503&rft.coden=TRSTAZ&rft_id=info:doi/10.1016/j.trstmh.2010.05.001&rft_dat=%3Cproquest_cross%3E754562337%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733981147&rft_id=info:pmid/20621218&rft_els_id=S0035920310001100&rfr_iscdi=true |