Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy

Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy

Gold‐containing chromatographic beads were used to record spectra of the tripeptide glutathione and the protein immunoglobulin G (IgG) by surface‐enhanced Raman scattering (SERS) spectroscopy. SERS spectra were recorded from glutathione in solution at three different pH values, 2, 6 and 10. It was f...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of Raman spectroscopy 2005-05, Vol.36 (5), p.394-399
Hauptverfasser: Larsson, Mina, Lindgren, Jan
Format: Artikel
Sprache:eng
Schlagworte:
chromatographic beads
glutathione
gold
immunoglobulin G
surface-enhanced Raman spectroscopy
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 399
container_issue 5
container_start_page 394
container_title Journal of Raman spectroscopy
container_volume 36
creator Larsson, Mina
Lindgren, Jan
description Gold‐containing chromatographic beads were used to record spectra of the tripeptide glutathione and the protein immunoglobulin G (IgG) by surface‐enhanced Raman scattering (SERS) spectroscopy. SERS spectra were recorded from glutathione in solution at three different pH values, 2, 6 and 10. It was found that the SERS spectra are strongly dependent on pH, which indicates that the adsorption mode of glutathione changes with the pH value of the solution. Assignment of some of the observed bands and a suggestion for adsorption modes at the different pH were made. A SERS spectrum of human IgG in ethanolamine was also recorded. No complete assignment of the bands in this spectrum was performed, but the results indicate adsorption to the gold nanoparticles by aromatic amino acids and cystine residues. Copyright © 2005 John Wiley & Sons, Ltd.
doi_str_mv 10.1002/jrs.1304
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754560257</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>754560257</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3354-619e22a7fa3a05a258d2244c97ab0d0320871aebe21d865c3f96f04eeb2f2b4a3</originalsourceid><addsrcrecordid>eNp10M1u1DAUhmELgdShIPUSvINNWv_GybIawUBbFTEtsLROnJMZl8RO7UR0Nlx7px2ExILV2Tw6-vQScsLZKWdMnN2lfMolUy_IgrPaFEpr_ZIsmDSmYKoqj8jrnO8YY3Vd8gX5fR6g32Wfaezopp8nmLY-BqQQWuqHYQ5x08dm7n2gK-pD9i1St01xgCluEoxb72iD0GY6Zx82NM-pA4cFhi0Ehy1dwwCBZgfThOlZjOimFLOL4-4NedVBn_Htn3tMvn38cLv8VFx9WX1enl8VTkqtipLXKASYDiQwDUJXrRBKudpAw1omBasMB2xQ8LYqtZNdXXZMITaiE40CeUzeHf6OKd7PmCc7-Oyw7yFgnLM1WumSCW328v1Buv3EnLCzY_IDpJ3lzD4VtvvC9qnwnhYH-sv3uPuvsxfrm3-9zxM-_PWQftrSSKPtj-uVra6X3y_X_Ktdykd7d48u</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>754560257</pqid></control><display><type>article</type><title>Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy</title><source>Wiley Online Library All Journals</source><creator>Larsson, Mina ; Lindgren, Jan</creator><creatorcontrib>Larsson, Mina ; Lindgren, Jan</creatorcontrib><description>Gold‐containing chromatographic beads were used to record spectra of the tripeptide glutathione and the protein immunoglobulin G (IgG) by surface‐enhanced Raman scattering (SERS) spectroscopy. SERS spectra were recorded from glutathione in solution at three different pH values, 2, 6 and 10. It was found that the SERS spectra are strongly dependent on pH, which indicates that the adsorption mode of glutathione changes with the pH value of the solution. Assignment of some of the observed bands and a suggestion for adsorption modes at the different pH were made. A SERS spectrum of human IgG in ethanolamine was also recorded. No complete assignment of the bands in this spectrum was performed, but the results indicate adsorption to the gold nanoparticles by aromatic amino acids and cystine residues. Copyright © 2005 John Wiley &amp; Sons, Ltd.</description><identifier>ISSN: 0377-0486</identifier><identifier>EISSN: 1097-4555</identifier><identifier>DOI: 10.1002/jrs.1304</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>chromatographic beads ; glutathione ; gold ; immunoglobulin G ; surface-enhanced Raman spectroscopy</subject><ispartof>Journal of Raman spectroscopy, 2005-05, Vol.36 (5), p.394-399</ispartof><rights>Copyright © 2005 John Wiley &amp; Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3354-619e22a7fa3a05a258d2244c97ab0d0320871aebe21d865c3f96f04eeb2f2b4a3</citedby><cites>FETCH-LOGICAL-c3354-619e22a7fa3a05a258d2244c97ab0d0320871aebe21d865c3f96f04eeb2f2b4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjrs.1304$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjrs.1304$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Larsson, Mina</creatorcontrib><creatorcontrib>Lindgren, Jan</creatorcontrib><title>Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy</title><title>Journal of Raman spectroscopy</title><addtitle>J. Raman Spectrosc</addtitle><description>Gold‐containing chromatographic beads were used to record spectra of the tripeptide glutathione and the protein immunoglobulin G (IgG) by surface‐enhanced Raman scattering (SERS) spectroscopy. SERS spectra were recorded from glutathione in solution at three different pH values, 2, 6 and 10. It was found that the SERS spectra are strongly dependent on pH, which indicates that the adsorption mode of glutathione changes with the pH value of the solution. Assignment of some of the observed bands and a suggestion for adsorption modes at the different pH were made. A SERS spectrum of human IgG in ethanolamine was also recorded. No complete assignment of the bands in this spectrum was performed, but the results indicate adsorption to the gold nanoparticles by aromatic amino acids and cystine residues. Copyright © 2005 John Wiley &amp; Sons, Ltd.</description><subject>chromatographic beads</subject><subject>glutathione</subject><subject>gold</subject><subject>immunoglobulin G</subject><subject>surface-enhanced Raman spectroscopy</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp10M1u1DAUhmELgdShIPUSvINNWv_GybIawUBbFTEtsLROnJMZl8RO7UR0Nlx7px2ExILV2Tw6-vQScsLZKWdMnN2lfMolUy_IgrPaFEpr_ZIsmDSmYKoqj8jrnO8YY3Vd8gX5fR6g32Wfaezopp8nmLY-BqQQWuqHYQ5x08dm7n2gK-pD9i1St01xgCluEoxb72iD0GY6Zx82NM-pA4cFhi0Ehy1dwwCBZgfThOlZjOimFLOL4-4NedVBn_Htn3tMvn38cLv8VFx9WX1enl8VTkqtipLXKASYDiQwDUJXrRBKudpAw1omBasMB2xQ8LYqtZNdXXZMITaiE40CeUzeHf6OKd7PmCc7-Oyw7yFgnLM1WumSCW328v1Buv3EnLCzY_IDpJ3lzD4VtvvC9qnwnhYH-sv3uPuvsxfrm3-9zxM-_PWQftrSSKPtj-uVra6X3y_X_Ktdykd7d48u</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Larsson, Mina</creator><creator>Lindgren, Jan</creator><general>John Wiley &amp; Sons, Ltd</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>200505</creationdate><title>Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy</title><author>Larsson, Mina ; Lindgren, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3354-619e22a7fa3a05a258d2244c97ab0d0320871aebe21d865c3f96f04eeb2f2b4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>chromatographic beads</topic><topic>glutathione</topic><topic>gold</topic><topic>immunoglobulin G</topic><topic>surface-enhanced Raman spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsson, Mina</creatorcontrib><creatorcontrib>Lindgren, Jan</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of Raman spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Larsson, Mina</au><au>Lindgren, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy</atitle><jtitle>Journal of Raman spectroscopy</jtitle><addtitle>J. Raman Spectrosc</addtitle><date>2005-05</date><risdate>2005</risdate><volume>36</volume><issue>5</issue><spage>394</spage><epage>399</epage><pages>394-399</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><abstract>Gold‐containing chromatographic beads were used to record spectra of the tripeptide glutathione and the protein immunoglobulin G (IgG) by surface‐enhanced Raman scattering (SERS) spectroscopy. SERS spectra were recorded from glutathione in solution at three different pH values, 2, 6 and 10. It was found that the SERS spectra are strongly dependent on pH, which indicates that the adsorption mode of glutathione changes with the pH value of the solution. Assignment of some of the observed bands and a suggestion for adsorption modes at the different pH were made. A SERS spectrum of human IgG in ethanolamine was also recorded. No complete assignment of the bands in this spectrum was performed, but the results indicate adsorption to the gold nanoparticles by aromatic amino acids and cystine residues. Copyright © 2005 John Wiley &amp; Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><doi>10.1002/jrs.1304</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0377-0486
ispartof Journal of Raman spectroscopy, 2005-05, Vol.36 (5), p.394-399
issn 0377-0486
1097-4555
language eng
recordid cdi_proquest_miscellaneous_754560257
source Wiley Online Library All Journals
subjects chromatographic beads
glutathione
gold
immunoglobulin G
surface-enhanced Raman spectroscopy
title Analysis of glutathione and immunoglobulin G inside chromatographic beads using surface-enhanced Raman scattering spectroscopy
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T12%3A52%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20glutathione%20and%20immunoglobulin%20G%20inside%20chromatographic%20beads%20using%20surface-enhanced%20Raman%20scattering%20spectroscopy&rft.jtitle=Journal%20of%20Raman%20spectroscopy&rft.au=Larsson,%20Mina&rft.date=2005-05&rft.volume=36&rft.issue=5&rft.spage=394&rft.epage=399&rft.pages=394-399&rft.issn=0377-0486&rft.eissn=1097-4555&rft_id=info:doi/10.1002/jrs.1304&rft_dat=%3Cproquest_cross%3E754560257%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=754560257&rft_id=info:pmid/&rfr_iscdi=true