Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants
The unicellular green alga Chlamydomonas reinhardtii is employed here for the setup of a biosensor demonstrator based on multibiomediators for the detection of herbicides. The detection is based on the activity of photosystem II, the multienzymatic chlorophyll-protein complex located in the thylakoi...
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| Veröffentlicht in: | Analytical & bioanalytical chemistry (Print) 2009-06, Vol.394 (4), p.1081-1087 |
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| creator | Scognamiglio, V Raffi, D Lambreva, M Rea, G Tibuzzi, A Pezzotti, G Johanningmeier, U Giardi, M. T |
| description | The unicellular green alga Chlamydomonas reinhardtii is employed here for the setup of a biosensor demonstrator based on multibiomediators for the detection of herbicides. The detection is based on the activity of photosystem II, the multienzymatic chlorophyll-protein complex located in the thylakoid membrane that catalyzes the light-dependent photosynthetic primary charge separation and the electron transfer chain in cyanobacteria, algae, and higher plants. Several C. reinhardtii mutants modified on the D1 photosystem II protein are generated by site-directed mutagenesis and experimentally tested for the development of a biosensor revealing the modification of the fluorescence parameter (1 - V J) in the presence of herbicides. The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. It follows that the response of the multibiomediators is associated to a particular herbicide subclass and can be useful to monitor several species of pollutants. |
| doi_str_mv | 10.1007/s00216-009-2668-1 |
| format | Article |
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The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. It follows that the response of the multibiomediators is associated to a particular herbicide subclass and can be useful to monitor several species of pollutants.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-009-2668-1</identifier><identifier>PMID: 19238365</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Algae ; Analytical Chemistry ; Animals ; Biochemistry ; Biological and medical sciences ; Biomediator ; Biosensing Techniques - methods ; Biosensor ; Biosensors ; Biotechnology ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - genetics ; Chlamydomonas reinhardtii - metabolism ; Cyanobacteria ; Environmental Pollutants - analysis ; Exact sciences and technology ; Fluorescence ; Food Science ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Genetic Variation ; Herbicides ; Herbicides - analysis ; Laboratory Medicine ; Methods. Procedures. 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T</creatorcontrib><title>Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants</title><title>Analytical & bioanalytical chemistry (Print)</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The unicellular green alga Chlamydomonas reinhardtii is employed here for the setup of a biosensor demonstrator based on multibiomediators for the detection of herbicides. The detection is based on the activity of photosystem II, the multienzymatic chlorophyll-protein complex located in the thylakoid membrane that catalyzes the light-dependent photosynthetic primary charge separation and the electron transfer chain in cyanobacteria, algae, and higher plants. Several C. reinhardtii mutants modified on the D1 photosystem II protein are generated by site-directed mutagenesis and experimentally tested for the development of a biosensor revealing the modification of the fluorescence parameter (1 - V J) in the presence of herbicides. The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. 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Several C. reinhardtii mutants modified on the D1 photosystem II protein are generated by site-directed mutagenesis and experimentally tested for the development of a biosensor revealing the modification of the fluorescence parameter (1 - V J) in the presence of herbicides. The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. It follows that the response of the multibiomediators is associated to a particular herbicide subclass and can be useful to monitor several species of pollutants.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19238365</pmid><doi>10.1007/s00216-009-2668-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Algae Analytical Chemistry Animals Biochemistry Biological and medical sciences Biomediator Biosensing Techniques - methods Biosensor Biosensors Biotechnology Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chlamydomonas reinhardtii Chlamydomonas reinhardtii - genetics Chlamydomonas reinhardtii - metabolism Cyanobacteria Environmental Pollutants - analysis Exact sciences and technology Fluorescence Food Science Fundamental and applied biological sciences. Psychology General, instrumentation Genetic Variation Herbicides Herbicides - analysis Laboratory Medicine Methods. Procedures. Technologies Monitoring/Environmental Analysis Monitors Original Paper Photosystem II Protein Complex - chemistry Photosystem II Protein Complex - metabolism Pollutants Sensitivity and Specificity site-directed mutagenesis Spectrometric and optical methods Time Factors Ureas Various methods and equipments |
| title | Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants |
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