Magneto-Optical Biosensing Platform Based on Light Scattering from Self-Assembled Chains of Functionalized Rotating Magnetic Beads
We describe a simple protocol for the rapid, highly sensitive, and quantitative measurement of the concentration of biomolecules in a solution by monitoring light scattered by self-assembled chains of functionalized superparamagnetic beads (SBs) rotating in the solution. A rotating external field (H...
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| Veröffentlicht in: | Nano letters 2010-02, Vol.10 (2), p.446-451 |
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| creator | Park, Sang Yoon Handa, Hiroshi Sandhu, Adarsh |
| description | We describe a simple protocol for the rapid, highly sensitive, and quantitative measurement of the concentration of biomolecules in a solution by monitoring light scattered by self-assembled chains of functionalized superparamagnetic beads (SBs) rotating in the solution. A rotating external field (H ex) applied to an aqueous solution containing 250 nm diameter biotinylated SBs produced linear chains of SBs rotating in phase with Hex due to magnetically induced self-assembly. At constant Hex, the addition of avidin to the solution led to the formation of longer SB-chains than without the presence of avidin. The generation of longer SB-chains was revealed by increases in the amplitude of the oscillating optical transmittance signal of the magnetic colloid solution. Monitoring changes in the amplitude of the optical transmittance of the solution enabled quantitative determination of the concentration of avidin added to the solution with a sensitivity of 100 pM (6.7 ng/mL) and a dynamic range of at least 3 orders of magnitude. The rotating chains acted as biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s. This approach offers a rapid, highly sensitive, inexpensive, and homogeneous means for detecting biorecognition processes. |
| doi_str_mv | 10.1021/nl9030488 |
| format | Article |
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A rotating external field (H ex) applied to an aqueous solution containing 250 nm diameter biotinylated SBs produced linear chains of SBs rotating in phase with Hex due to magnetically induced self-assembly. At constant Hex, the addition of avidin to the solution led to the formation of longer SB-chains than without the presence of avidin. The generation of longer SB-chains was revealed by increases in the amplitude of the oscillating optical transmittance signal of the magnetic colloid solution. Monitoring changes in the amplitude of the optical transmittance of the solution enabled quantitative determination of the concentration of avidin added to the solution with a sensitivity of 100 pM (6.7 ng/mL) and a dynamic range of at least 3 orders of magnitude. The rotating chains acted as biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s. This approach offers a rapid, highly sensitive, inexpensive, and homogeneous means for detecting biorecognition processes.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl9030488</identifier><identifier>PMID: 20038151</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Avidin - chemistry ; Biological Assay - instrumentation ; Biological Assay - methods ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biotechnology - methods ; Condensed matter: electronic structure, electrical, magnetic, and optical properties ; Cross-disciplinary physics: materials science; rheology ; DNA, Complementary - chemistry ; Enzyme-Linked Immunosorbent Assay - methods ; Exact sciences and technology ; Fullerenes and related materials ; General equipment and techniques ; Growth from solutions ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Light ; Magnetics ; Materials science ; Methods of crystal growth; physics of crystal growth ; Methods of nanofabrication ; Nucleic Acid Hybridization ; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation ; Optics and Photonics ; Physics ; Scattering, Radiation ; Self-assembly ; Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing ; Visible and ultraviolet spectra</subject><ispartof>Nano letters, 2010-02, Vol.10 (2), p.446-451</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a486t-fd69c1a476d471a05a2f852f3276952b05f2eafcf1c7e662f8c3dc3aaf0b20863</citedby><cites>FETCH-LOGICAL-a486t-fd69c1a476d471a05a2f852f3276952b05f2eafcf1c7e662f8c3dc3aaf0b20863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nl9030488$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nl9030488$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22428578$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20038151$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sang Yoon</creatorcontrib><creatorcontrib>Handa, Hiroshi</creatorcontrib><creatorcontrib>Sandhu, Adarsh</creatorcontrib><title>Magneto-Optical Biosensing Platform Based on Light Scattering from Self-Assembled Chains of Functionalized Rotating Magnetic Beads</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>We describe a simple protocol for the rapid, highly sensitive, and quantitative measurement of the concentration of biomolecules in a solution by monitoring light scattered by self-assembled chains of functionalized superparamagnetic beads (SBs) rotating in the solution. A rotating external field (H ex) applied to an aqueous solution containing 250 nm diameter biotinylated SBs produced linear chains of SBs rotating in phase with Hex due to magnetically induced self-assembly. At constant Hex, the addition of avidin to the solution led to the formation of longer SB-chains than without the presence of avidin. The generation of longer SB-chains was revealed by increases in the amplitude of the oscillating optical transmittance signal of the magnetic colloid solution. Monitoring changes in the amplitude of the optical transmittance of the solution enabled quantitative determination of the concentration of avidin added to the solution with a sensitivity of 100 pM (6.7 ng/mL) and a dynamic range of at least 3 orders of magnitude. The rotating chains acted as biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s. This approach offers a rapid, highly sensitive, inexpensive, and homogeneous means for detecting biorecognition processes.</description><subject>Avidin - chemistry</subject><subject>Biological Assay - instrumentation</subject><subject>Biological Assay - methods</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biotechnology - methods</subject><subject>Condensed matter: electronic structure, electrical, magnetic, and optical properties</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>DNA, Complementary - chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Exact sciences and technology</subject><subject>Fullerenes and related materials</subject><subject>General equipment and techniques</subject><subject>Growth from solutions</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Light</subject><subject>Magnetics</subject><subject>Materials science</subject><subject>Methods of crystal growth; physics of crystal growth</subject><subject>Methods of nanofabrication</subject><subject>Nucleic Acid Hybridization</subject><subject>Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation</subject><subject>Optics and Photonics</subject><subject>Physics</subject><subject>Scattering, Radiation</subject><subject>Self-assembly</subject><subject>Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing</subject><subject>Visible and ultraviolet spectra</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBuAIgWgpHPgDyBdUcUjxZ-IcuysKSIuKaHuOZh1768qxF49zgCO_nKx22V4qcZqR5vGM5Leq3jJ6wShnH2PoqKBS62fVKVOC1k3X8efHXsuT6hXiA6W0E4q-rE44pUIzxU6rP99gE21J9fW2eAOBLHxCG9HHDfkeoLiUR7IAtANJkaz85r6QGwOl2LwjLqeR3Njg6ktEO67D7Jb34COS5MjVFE3xKULwv-fBj1Sg7F7tb3pDFhYGfF29cBDQvjnUs-ru6tPt8ku9uv78dXm5qkHqptRuaDrDQLbNIFsGVAF3WnEneNt0iq-pctyCM46Z1jbNPDRiMALA0TWnuhFn1fl-7zann5PF0o8ejQ0Bok0T9q2SSnadpP-XQvJWc8pn-WEvTU6I2bp-m_0I-VfPaL_Lpj9mM9t3h63TerTDUf4LYwbvDwBwjsJliMbjo-OSa9XqRwcG-4c05fmD8YmDfwGNqaLg</recordid><startdate>20100210</startdate><enddate>20100210</enddate><creator>Park, Sang Yoon</creator><creator>Handa, Hiroshi</creator><creator>Sandhu, Adarsh</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100210</creationdate><title>Magneto-Optical Biosensing Platform Based on Light Scattering from Self-Assembled Chains of Functionalized Rotating Magnetic Beads</title><author>Park, Sang Yoon ; Handa, Hiroshi ; Sandhu, Adarsh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a486t-fd69c1a476d471a05a2f852f3276952b05f2eafcf1c7e662f8c3dc3aaf0b20863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Avidin - chemistry</topic><topic>Biological Assay - instrumentation</topic><topic>Biological Assay - methods</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biotechnology - methods</topic><topic>Condensed matter: electronic structure, electrical, magnetic, and optical properties</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>DNA, Complementary - chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Exact sciences and technology</topic><topic>Fullerenes and related materials</topic><topic>General equipment and techniques</topic><topic>Growth from solutions</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>Light</topic><topic>Magnetics</topic><topic>Materials science</topic><topic>Methods of crystal growth; physics of crystal growth</topic><topic>Methods of nanofabrication</topic><topic>Nucleic Acid Hybridization</topic><topic>Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation</topic><topic>Optics and Photonics</topic><topic>Physics</topic><topic>Scattering, Radiation</topic><topic>Self-assembly</topic><topic>Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing</topic><topic>Visible and ultraviolet spectra</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sang Yoon</creatorcontrib><creatorcontrib>Handa, Hiroshi</creatorcontrib><creatorcontrib>Sandhu, Adarsh</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sang Yoon</au><au>Handa, Hiroshi</au><au>Sandhu, Adarsh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magneto-Optical Biosensing Platform Based on Light Scattering from Self-Assembled Chains of Functionalized Rotating Magnetic Beads</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2010-02-10</date><risdate>2010</risdate><volume>10</volume><issue>2</issue><spage>446</spage><epage>451</epage><pages>446-451</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We describe a simple protocol for the rapid, highly sensitive, and quantitative measurement of the concentration of biomolecules in a solution by monitoring light scattered by self-assembled chains of functionalized superparamagnetic beads (SBs) rotating in the solution. A rotating external field (H ex) applied to an aqueous solution containing 250 nm diameter biotinylated SBs produced linear chains of SBs rotating in phase with Hex due to magnetically induced self-assembly. At constant Hex, the addition of avidin to the solution led to the formation of longer SB-chains than without the presence of avidin. The generation of longer SB-chains was revealed by increases in the amplitude of the oscillating optical transmittance signal of the magnetic colloid solution. Monitoring changes in the amplitude of the optical transmittance of the solution enabled quantitative determination of the concentration of avidin added to the solution with a sensitivity of 100 pM (6.7 ng/mL) and a dynamic range of at least 3 orders of magnitude. The rotating chains acted as biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s. This approach offers a rapid, highly sensitive, inexpensive, and homogeneous means for detecting biorecognition processes.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20038151</pmid><doi>10.1021/nl9030488</doi><tpages>6</tpages></addata></record> |
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| subjects | Avidin - chemistry Biological Assay - instrumentation Biological Assay - methods Biosensing Techniques - instrumentation Biosensing Techniques - methods Biotechnology - methods Condensed matter: electronic structure, electrical, magnetic, and optical properties Cross-disciplinary physics: materials science rheology DNA, Complementary - chemistry Enzyme-Linked Immunosorbent Assay - methods Exact sciences and technology Fullerenes and related materials General equipment and techniques Growth from solutions Instruments, apparatus, components and techniques common to several branches of physics and astronomy Light Magnetics Materials science Methods of crystal growth physics of crystal growth Methods of nanofabrication Nucleic Acid Hybridization Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation Optics and Photonics Physics Scattering, Radiation Self-assembly Sensors (chemical, optical, electrical, movement, gas, etc.) remote sensing Visible and ultraviolet spectra |
| title | Magneto-Optical Biosensing Platform Based on Light Scattering from Self-Assembled Chains of Functionalized Rotating Magnetic Beads |
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