Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Complementary bioconjugates based on antibody−antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjug...

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Veröffentlicht in:Nano letters 2002-08, Vol.2 (8), p.817-822
Hauptverfasser: Wang, Shaopeng, Mamedova, Natalia, Kotov, Nicholas A, Chen, Wei, Studer, Joe
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container_title Nano letters
container_volume 2
creator Wang, Shaopeng
Mamedova, Natalia
Kotov, Nicholas A
Chen, Wei
Studer, Joe
description Complementary bioconjugates based on antibody−antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS−PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen−antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA−IgG immunocomplex resulted in the Förster resonance energy transfer (FRET) between the two different NPs:  the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.
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