Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas
Background. An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC). Methods. In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders...
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| Veröffentlicht in: | Head & neck 2010-02, Vol.32 (2), p.229-234 |
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| creator | Chuang, Tony C. Y. Chuang, Alice Y. C. Poeta, Luana Koch, Wayne M. Califano, Joseph A. Tufano, Ralph P. |
| description | Background.
An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC).
Methods.
In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for BRAF mutation using a gap‐ligase chain reaction technique.
Results.
The BRAF mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the BRAF mutation. Moreover, 3 of 14 patients with PTC were positive for BRAF mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis.
Conclusions.
The detection of free circulating mutant BRAF in patients with PTC is possible and future studies are warranted to determine its clinical significance. © 2009 Wiley Periodicals, Inc. Head Neck, 2010 |
| doi_str_mv | 10.1002/hed.21178 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754549538</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>744716073</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4568-e1eb934ff4cfec82b4477680d828cbd54a2e761a8b8d474a0450ab087dec22be3</originalsourceid><addsrcrecordid>eNqF0ctuEzEUBmALgWgpLHgB5A1CLKb13c4ybZoEVBWJ6wpZHs8ZxTA37Bm1efs6JGSHuvJF3znH8o_Qa0rOKSHsYgPVOaNUmyfolJKZLggX-uluL3jBiRYn6EVKvwghXAn2HJ3QmWJKcXmKfi5gBD-6sgF8-Xm-xO00ujH0HQ4dThCnFi9u5zi5dmgg4Tr2LR4ygG5M-C6Mm3waQtO4uMXjZhv7UGHvog9d37r0Ej2rXZPg1WE9Q9-W11-v1sXNp9WHq_lN4YVUpgAK5YyLuha-Bm9YKYTWypDKMOPLSgrHQCvqTGkqoYUjQhJXEqMr8IyVwM_Qu33fIfZ_JkijbUPykJ_VQT8lq6WQYia5eVzm0VQRzR-XnBsijdrJ93vpY59ShNoOMbT5RywldheQzQHZvwFl--bQdSrbfHuUh0QyeHsALnnX1NF1PqSjY4wZwszOXezdXWhg-_-Jdn29-De62FeENML9scLF31ZprqX9cbuyH9ffV0utvljJHwA2nbV_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733805863</pqid></control><display><type>article</type><title>Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas</title><source>MEDLINE</source><source>Wiley Online Library</source><creator>Chuang, Tony C. Y. ; Chuang, Alice Y. C. ; Poeta, Luana ; Koch, Wayne M. ; Califano, Joseph A. ; Tufano, Ralph P.</creator><creatorcontrib>Chuang, Tony C. Y. ; Chuang, Alice Y. C. ; Poeta, Luana ; Koch, Wayne M. ; Califano, Joseph A. ; Tufano, Ralph P.</creatorcontrib><description>Background.
An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC).
Methods.
In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for BRAF mutation using a gap‐ligase chain reaction technique.
Results.
The BRAF mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the BRAF mutation. Moreover, 3 of 14 patients with PTC were positive for BRAF mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis.
Conclusions.
The detection of free circulating mutant BRAF in patients with PTC is possible and future studies are warranted to determine its clinical significance. © 2009 Wiley Periodicals, Inc. Head Neck, 2010</description><identifier>ISSN: 1043-3074</identifier><identifier>EISSN: 1097-0347</identifier><identifier>DOI: 10.1002/hed.21178</identifier><identifier>PMID: 19626635</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; biomarker ; BRAF ; Carcinoma, Papillary - genetics ; Carcinoma, Papillary, Follicular - genetics ; DNA, Neoplasm - blood ; DNA, Neoplasm - genetics ; Endocrinopathies ; Exons ; Female ; Hashimoto Disease ; Humans ; Lymphatic Metastasis ; Male ; Malignant tumors ; Medical sciences ; Middle Aged ; Mutation ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Otorhinolaryngology. Stomatology ; Polymerase Chain Reaction ; Proto-Oncogene Proteins B-raf - blood ; Proto-Oncogene Proteins B-raf - genetics ; serum DNA ; thyroid carcinoma ; Thyroid Neoplasms - genetics ; Thyroid. Thyroid axis (diseases)</subject><ispartof>Head & neck, 2010-02, Vol.32 (2), p.229-234</ispartof><rights>Copyright © 2009 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2009 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4568-e1eb934ff4cfec82b4477680d828cbd54a2e761a8b8d474a0450ab087dec22be3</citedby><cites>FETCH-LOGICAL-c4568-e1eb934ff4cfec82b4477680d828cbd54a2e761a8b8d474a0450ab087dec22be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhed.21178$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhed.21178$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22280285$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19626635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chuang, Tony C. Y.</creatorcontrib><creatorcontrib>Chuang, Alice Y. C.</creatorcontrib><creatorcontrib>Poeta, Luana</creatorcontrib><creatorcontrib>Koch, Wayne M.</creatorcontrib><creatorcontrib>Califano, Joseph A.</creatorcontrib><creatorcontrib>Tufano, Ralph P.</creatorcontrib><title>Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas</title><title>Head & neck</title><addtitle>Head Neck</addtitle><description>Background.
An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC).
Methods.
In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for BRAF mutation using a gap‐ligase chain reaction technique.
Results.
The BRAF mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the BRAF mutation. Moreover, 3 of 14 patients with PTC were positive for BRAF mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis.
Conclusions.
The detection of free circulating mutant BRAF in patients with PTC is possible and future studies are warranted to determine its clinical significance. © 2009 Wiley Periodicals, Inc. Head Neck, 2010</description><subject>Biological and medical sciences</subject><subject>biomarker</subject><subject>BRAF</subject><subject>Carcinoma, Papillary - genetics</subject><subject>Carcinoma, Papillary, Follicular - genetics</subject><subject>DNA, Neoplasm - blood</subject><subject>DNA, Neoplasm - genetics</subject><subject>Endocrinopathies</subject><subject>Exons</subject><subject>Female</subject><subject>Hashimoto Disease</subject><subject>Humans</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Polymerase Chain Reaction</subject><subject>Proto-Oncogene Proteins B-raf - blood</subject><subject>Proto-Oncogene Proteins B-raf - genetics</subject><subject>serum DNA</subject><subject>thyroid carcinoma</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>1043-3074</issn><issn>1097-0347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctuEzEUBmALgWgpLHgB5A1CLKb13c4ybZoEVBWJ6wpZHs8ZxTA37Bm1efs6JGSHuvJF3znH8o_Qa0rOKSHsYgPVOaNUmyfolJKZLggX-uluL3jBiRYn6EVKvwghXAn2HJ3QmWJKcXmKfi5gBD-6sgF8-Xm-xO00ujH0HQ4dThCnFi9u5zi5dmgg4Tr2LR4ygG5M-C6Mm3waQtO4uMXjZhv7UGHvog9d37r0Ej2rXZPg1WE9Q9-W11-v1sXNp9WHq_lN4YVUpgAK5YyLuha-Bm9YKYTWypDKMOPLSgrHQCvqTGkqoYUjQhJXEqMr8IyVwM_Qu33fIfZ_JkijbUPykJ_VQT8lq6WQYia5eVzm0VQRzR-XnBsijdrJ93vpY59ShNoOMbT5RywldheQzQHZvwFl--bQdSrbfHuUh0QyeHsALnnX1NF1PqSjY4wZwszOXezdXWhg-_-Jdn29-De62FeENML9scLF31ZprqX9cbuyH9ffV0utvljJHwA2nbV_</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Chuang, Tony C. Y.</creator><creator>Chuang, Alice Y. C.</creator><creator>Poeta, Luana</creator><creator>Koch, Wayne M.</creator><creator>Califano, Joseph A.</creator><creator>Tufano, Ralph P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>201002</creationdate><title>Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas</title><author>Chuang, Tony C. Y. ; Chuang, Alice Y. C. ; Poeta, Luana ; Koch, Wayne M. ; Califano, Joseph A. ; Tufano, Ralph P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4568-e1eb934ff4cfec82b4477680d828cbd54a2e761a8b8d474a0450ab087dec22be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>biomarker</topic><topic>BRAF</topic><topic>Carcinoma, Papillary - genetics</topic><topic>Carcinoma, Papillary, Follicular - genetics</topic><topic>DNA, Neoplasm - blood</topic><topic>DNA, Neoplasm - genetics</topic><topic>Endocrinopathies</topic><topic>Exons</topic><topic>Female</topic><topic>Hashimoto Disease</topic><topic>Humans</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Polymerase Chain Reaction</topic><topic>Proto-Oncogene Proteins B-raf - blood</topic><topic>Proto-Oncogene Proteins B-raf - genetics</topic><topic>serum DNA</topic><topic>thyroid carcinoma</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chuang, Tony C. Y.</creatorcontrib><creatorcontrib>Chuang, Alice Y. C.</creatorcontrib><creatorcontrib>Poeta, Luana</creatorcontrib><creatorcontrib>Koch, Wayne M.</creatorcontrib><creatorcontrib>Califano, Joseph A.</creatorcontrib><creatorcontrib>Tufano, Ralph P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Head & neck</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chuang, Tony C. Y.</au><au>Chuang, Alice Y. C.</au><au>Poeta, Luana</au><au>Koch, Wayne M.</au><au>Califano, Joseph A.</au><au>Tufano, Ralph P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas</atitle><jtitle>Head & neck</jtitle><addtitle>Head Neck</addtitle><date>2010-02</date><risdate>2010</risdate><volume>32</volume><issue>2</issue><spage>229</spage><epage>234</epage><pages>229-234</pages><issn>1043-3074</issn><eissn>1097-0347</eissn><abstract>Background.
An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC).
Methods.
In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for BRAF mutation using a gap‐ligase chain reaction technique.
Results.
The BRAF mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the BRAF mutation. Moreover, 3 of 14 patients with PTC were positive for BRAF mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis.
Conclusions.
The detection of free circulating mutant BRAF in patients with PTC is possible and future studies are warranted to determine its clinical significance. © 2009 Wiley Periodicals, Inc. Head Neck, 2010</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19626635</pmid><doi>10.1002/hed.21178</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences biomarker BRAF Carcinoma, Papillary - genetics Carcinoma, Papillary, Follicular - genetics DNA, Neoplasm - blood DNA, Neoplasm - genetics Endocrinopathies Exons Female Hashimoto Disease Humans Lymphatic Metastasis Male Malignant tumors Medical sciences Middle Aged Mutation Non tumoral diseases. Target tissue resistance. Benign neoplasms Otorhinolaryngology. Stomatology Polymerase Chain Reaction Proto-Oncogene Proteins B-raf - blood Proto-Oncogene Proteins B-raf - genetics serum DNA thyroid carcinoma Thyroid Neoplasms - genetics Thyroid. Thyroid axis (diseases) |
| title | Detectable BRAF mutation in serum DNA samples from patients with papillary thyroid carcinomas |
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