Microgel-Based Inks for Paper-Supported Biosensing Applications
As a first step for the development of biosensing inks for inexpensive paper-based biodetection, we prepared paper strips printed with carboxylic poly(N-isopropylacrylamide) microgels that were modified either with an antibody or with a DNA aptamer. We found that the antibody and the DNA aptamer ret...
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| Veröffentlicht in: | Bioconjugate chemistry 2008-03, Vol.9 (3), p.935-941 |
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| container_issue | 3 |
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| container_title | Bioconjugate chemistry |
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| creator | Su, Shunxing Ali, Md. Monsur Filipe, Carlos D. M Li, Yingfu Pelton, Robert |
| description | As a first step for the development of biosensing inks for inexpensive paper-based biodetection, we prepared paper strips printed with carboxylic poly(N-isopropylacrylamide) microgels that were modified either with an antibody or with a DNA aptamer. We found that the antibody and the DNA aptamer retained their recognition capabilities when coupled to microgel. The printed microgel remains stationary during chromatographic elution while the microgel-supported molecular recognition elements are accessible to their intended targets present in the elution solution. Our work indicates that microgels, large enough to isolate the biosensors from the paper surface, are sufficiently hydrophilic to be wetted during chromatographic elution, exposing the gel-supported affinity probes to their targets. |
| doi_str_mv | 10.1021/bm7013608 |
| format | Article |
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Monsur</creatorcontrib><creatorcontrib>Filipe, Carlos D. M</creatorcontrib><creatorcontrib>Li, Yingfu</creatorcontrib><creatorcontrib>Pelton, Robert</creatorcontrib><title>Microgel-Based Inks for Paper-Supported Biosensing Applications</title><title>Bioconjugate chemistry</title><addtitle>Biomacromolecules</addtitle><description>As a first step for the development of biosensing inks for inexpensive paper-based biodetection, we prepared paper strips printed with carboxylic poly(N-isopropylacrylamide) microgels that were modified either with an antibody or with a DNA aptamer. We found that the antibody and the DNA aptamer retained their recognition capabilities when coupled to microgel. The printed microgel remains stationary during chromatographic elution while the microgel-supported molecular recognition elements are accessible to their intended targets present in the elution solution. Our work indicates that microgels, large enough to isolate the biosensors from the paper surface, are sufficiently hydrophilic to be wetted during chromatographic elution, exposing the gel-supported affinity probes to their targets.</description><subject>Acrylamides - chemistry</subject><subject>Acrylic Resins</subject><subject>Adenosine Triphosphate - analysis</subject><subject>Antibodies - chemistry</subject><subject>Antigens - analysis</subject><subject>Applied sciences</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chromatography, Paper</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels - chemistry</subject><subject>Ink</subject><subject>Methods. Procedures. Technologies</subject><subject>Organic polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polymers - chemistry</subject><subject>Properties and characterization</subject><subject>Solution and gel properties</subject><subject>Various methods and equipments</subject><issn>1525-7797</issn><issn>1043-1802</issn><issn>1526-4602</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0TtPwzAQB3ALgWgpDHwBlAUQQ-D8iuMJtRWPSkUgAXPkuHaVkhd2MvDtSWnULggmn-Sf7k7_Q-gUwzUGgm_SQgCmEcR7aIg5iUIWAdn_qXkohBQDdOT9CgAkZfwQDXBMJJVAhuj2KdOuWpo8nChvFsGs_PCBrVzwomrjwte2rivXdB-TrPKm9Fm5DMZ1nWdaNVlV-mN0YFXuzUn_jtD7_d3b9DGcPz_MpuN5qBhETciZpMZwwa2VsSaxWjAsjYm6RQyxXIKOIikwI1RSiLGWKSiuhJQqtYZwoCN0uelbu-qzNb5Jisxrk-eqNFXrE8EZZzGPo05e_C2BxpiK_yEBIUDINbzawC4p752xSe2yQrmvBEOyPkCyPUBnz_qmbVqYxU72iXfgvAfKa5Vbp0qd-a0jgCUAFzuntE9WVevKLt5fBn4D752WZQ</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Su, Shunxing</creator><creator>Ali, Md. 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Technologies</topic><topic>Organic polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polymers - chemistry</topic><topic>Properties and characterization</topic><topic>Solution and gel properties</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Shunxing</creatorcontrib><creatorcontrib>Ali, Md. Monsur</creatorcontrib><creatorcontrib>Filipe, Carlos D. 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| identifier | ISSN: 1525-7797 |
| ispartof | Bioconjugate chemistry, 2008-03, Vol.9 (3), p.935-941 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Acrylamides - chemistry Acrylic Resins Adenosine Triphosphate - analysis Antibodies - chemistry Antigens - analysis Applied sciences Aptamers, Nucleotide - chemistry Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Chromatography, Paper Exact sciences and technology Fundamental and applied biological sciences. Psychology Gels - chemistry Ink Methods. Procedures. Technologies Organic polymers Physicochemistry of polymers Polymers - chemistry Properties and characterization Solution and gel properties Various methods and equipments |
| title | Microgel-Based Inks for Paper-Supported Biosensing Applications |
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