Effect of Hydrophobic Interactions on Properties and Stability of DNA−Polyelectrolyte Complexes
Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spect...
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| Veröffentlicht in: | Langmuir 2010-04, Vol.26 (7), p.4999-5006 |
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| creator | Filippov, Sergey K Koňák, Čestmír Kopečková, Pavla Starovoytova, Larisa Špírková, Milena Štěpánek, Petr |
| description | Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. Dynamic, static, and electrophoretic light scattering, small-angle X-ray scattering, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30−60 nm; they were related to the polycation/DNA ratio and hydrophobicity of the used polycations (the cholesterol content). The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions. |
| doi_str_mv | 10.1021/la9036716 |
| format | Article |
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We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. Dynamic, static, and electrophoretic light scattering, small-angle X-ray scattering, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30−60 nm; they were related to the polycation/DNA ratio and hydrophobicity of the used polycations (the cholesterol content). The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/la9036716</identifier><identifier>PMID: 20073519</identifier><identifier>CODEN: LANGD5</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials ; Chemistry ; Colloidal state and disperse state ; DNA - chemistry ; Exact sciences and technology ; General and physical chemistry ; Hydrophobic and Hydrophilic Interactions ; Kinetics ; Magnetic Resonance Spectroscopy ; Microscopy, Atomic Force ; Models, Theoretical ; Molecular Structure ; Polyamines - chemistry ; Scattering, Radiation ; Spectrophotometry, Ultraviolet ; Surface physical chemistry</subject><ispartof>Langmuir, 2010-04, Vol.26 (7), p.4999-5006</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-1e2633488ef2f9c7fcdca921264b53bfb4ce0f99efc00d1cf70a6e43e7833db13</citedby><cites>FETCH-LOGICAL-a442t-1e2633488ef2f9c7fcdca921264b53bfb4ce0f99efc00d1cf70a6e43e7833db13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/la9036716$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/la9036716$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22581916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20073519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filippov, Sergey K</creatorcontrib><creatorcontrib>Koňák, Čestmír</creatorcontrib><creatorcontrib>Kopečková, Pavla</creatorcontrib><creatorcontrib>Starovoytova, Larisa</creatorcontrib><creatorcontrib>Špírková, Milena</creatorcontrib><creatorcontrib>Štěpánek, Petr</creatorcontrib><title>Effect of Hydrophobic Interactions on Properties and Stability of DNA−Polyelectrolyte Complexes</title><title>Langmuir</title><addtitle>Langmuir</addtitle><description>Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. Dynamic, static, and electrophoretic light scattering, small-angle X-ray scattering, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30−60 nm; they were related to the polycation/DNA ratio and hydrophobicity of the used polycations (the cholesterol content). The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.</description><subject>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>DNA - chemistry</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Kinetics</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Microscopy, Atomic Force</subject><subject>Models, Theoretical</subject><subject>Molecular Structure</subject><subject>Polyamines - chemistry</subject><subject>Scattering, Radiation</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Surface physical chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUQIMoWh8Lf0BmI-JiNK-ZTJalVlsoWlDXQyZzgyPppCYp2D9w7Sf6JY621o3gKhdy7rlwEDom-IJgSi6tkpjlguRbqEcyitOsoGIb9bDgLBU8Z3toP4RnjLFkXO6iPYqxYBmRPaSGxoCOiTPJaFl7N39yVaOTcRvBKx0b14bEtcm0-wEfGwiJauvkPqqqsU1cfu1d3fY_3t6nzi7BdirfDRGSgZvNLbxCOEQ7RtkAR-v3AD1eDx8Go3RydzMe9Cep4pzGlADNGeNFAYYaqYXRtVaSEprzKmOVqbgGbKQEozGuiTYCqxw4A1EwVleEHaCzlXfu3csCQixnTdBgrWrBLUIpMp7xgkr-P8kY61ox2ZHnK1J7F4IHU859M1N-WRJcfqUvN-k79mRtXVQzqDfkT-sOOF0DKmhljVetbsIvR7OCyG_RmlM6lM9u4dsu2x8HPwG7MJiM</recordid><startdate>20100406</startdate><enddate>20100406</enddate><creator>Filippov, Sergey K</creator><creator>Koňák, Čestmír</creator><creator>Kopečková, Pavla</creator><creator>Starovoytova, Larisa</creator><creator>Špírková, Milena</creator><creator>Štěpánek, Petr</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20100406</creationdate><title>Effect of Hydrophobic Interactions on Properties and Stability of DNA−Polyelectrolyte Complexes</title><author>Filippov, Sergey K ; Koňák, Čestmír ; Kopečková, Pavla ; Starovoytova, Larisa ; Špírková, Milena ; Štěpánek, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-1e2633488ef2f9c7fcdca921264b53bfb4ce0f99efc00d1cf70a6e43e7833db13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials</topic><topic>Chemistry</topic><topic>Colloidal state and disperse state</topic><topic>DNA - chemistry</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Kinetics</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Microscopy, Atomic Force</topic><topic>Models, Theoretical</topic><topic>Molecular Structure</topic><topic>Polyamines - chemistry</topic><topic>Scattering, Radiation</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Surface physical chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filippov, Sergey K</creatorcontrib><creatorcontrib>Koňák, Čestmír</creatorcontrib><creatorcontrib>Kopečková, Pavla</creatorcontrib><creatorcontrib>Starovoytova, Larisa</creatorcontrib><creatorcontrib>Špírková, Milena</creatorcontrib><creatorcontrib>Štěpánek, Petr</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filippov, Sergey K</au><au>Koňák, Čestmír</au><au>Kopečková, Pavla</au><au>Starovoytova, Larisa</au><au>Špírková, Milena</au><au>Štěpánek, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Hydrophobic Interactions on Properties and Stability of DNA−Polyelectrolyte Complexes</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2010-04-06</date><risdate>2010</risdate><volume>26</volume><issue>7</issue><spage>4999</spage><epage>5006</epage><pages>4999-5006</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. Dynamic, static, and electrophoretic light scattering, small-angle X-ray scattering, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30−60 nm; they were related to the polycation/DNA ratio and hydrophobicity of the used polycations (the cholesterol content). The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20073519</pmid><doi>10.1021/la9036716</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials Chemistry Colloidal state and disperse state DNA - chemistry Exact sciences and technology General and physical chemistry Hydrophobic and Hydrophilic Interactions Kinetics Magnetic Resonance Spectroscopy Microscopy, Atomic Force Models, Theoretical Molecular Structure Polyamines - chemistry Scattering, Radiation Spectrophotometry, Ultraviolet Surface physical chemistry |
| title | Effect of Hydrophobic Interactions on Properties and Stability of DNA−Polyelectrolyte Complexes |
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