Antigen Binding Forces of Single Antilysozyme Fv Fragments Explored by Atomic Force Microscopy

We used atomic force microscopy (AFM) to explore the antigen binding forces of individual Fv fragments of antilysozyme antibodies (Fv). To detect single molecular recognition events, genetically engineered histidine-tagged Fv fragments were coupled onto AFM tips modified with mixed self-assembled mo...

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Veröffentlicht in:	Langmuir 2005-06, Vol.21 (12), p.5517-5523
Hauptverfasser:	Berquand, Alexandre, Xia, Nan, Castner, David G, Clare, Brian H, Abbott, Nicholas L, Dupres, Vincent, Adriaensen, Yasmine, Dufrêne, Yves F
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigen-Antibody Complex - chemistry Antigen-Antibody Reactions Chemistry Exact sciences and technology General and physical chemistry Immunoglobulin Fragments - chemistry Microscopy, Atomic Force - methods Muramidase - chemistry Muramidase - immunology Surface Properties
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