Chemical Engineering of Mesenchymal Stem Cells to Induce a Cell Rolling Response
Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin−streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX o...
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| Veröffentlicht in: | Bioconjugate chemistry 2008-11, Vol.19 (11), p.2105-2109 |
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| container_end_page | 2109 |
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| container_issue | 11 |
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| container_title | Bioconjugate chemistry |
| container_volume | 19 |
| creator | Sarkar, Debanjan Vemula, Praveen Kumar Teo, Grace S. L Spelke, Dawn Karnik, Rohit Wee, Le Y Karp, Jeffrey M |
| description | Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin−streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue. |
| doi_str_mv | 10.1021/bc800345q |
| format | Article |
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These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.</description><identifier>ISSN: 1043-1802</identifier><identifier>EISSN: 1520-4812</identifier><identifier>DOI: 10.1021/bc800345q</identifier><identifier>PMID: 18973352</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biotin - metabolism ; Carbohydrates ; Cell Differentiation ; Cell Survival ; Chemical engineering ; Fluorescence ; Genotype & phenotype ; Humans ; Leukocyte Rolling - physiology ; Leukocytes ; Mesenchymal Stromal Cells - chemistry ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Oligosaccharides - chemistry ; P-Selectin - metabolism ; Sensitivity and Specificity ; Staining and Labeling ; Stem cells ; Streptavidin - metabolism ; Tissues</subject><ispartof>Bioconjugate chemistry, 2008-11, Vol.19 (11), p.2105-2109</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>Copyright American Chemical Society Nov 19, 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a476t-c44cc1c5d963f210fac1666595c5b648803292522f8f30f17aef677c03b60f883</citedby><cites>FETCH-LOGICAL-a476t-c44cc1c5d963f210fac1666595c5b648803292522f8f30f17aef677c03b60f883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bc800345q$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bc800345q$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18973352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarkar, Debanjan</creatorcontrib><creatorcontrib>Vemula, Praveen Kumar</creatorcontrib><creatorcontrib>Teo, Grace S. 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These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.</description><subject>Biotin - metabolism</subject><subject>Carbohydrates</subject><subject>Cell Differentiation</subject><subject>Cell Survival</subject><subject>Chemical engineering</subject><subject>Fluorescence</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Leukocyte Rolling - physiology</subject><subject>Leukocytes</subject><subject>Mesenchymal Stromal Cells - chemistry</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Oligosaccharides - chemistry</subject><subject>P-Selectin - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Staining and Labeling</subject><subject>Stem cells</subject><subject>Streptavidin - metabolism</subject><subject>Tissues</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90UtrGzEQAGBRWvI-9A-EJdCUHrYZvbXHYhI3kNDEdqE3IcujZJN9OKtdiP99ldo4kEBPEppPmtEMIZ8pfKfA6NncGwAu5NMHskclg1wYyj6mPQieUwNsl-zH-AAABTVsh-xSU2jOJdsjN6N7rEvvquy8uSsbxK5s7rI2ZNcYsfH3qzqFpj3W2QirKmZ9m102i8Fj5v6dZJO2ql6uTDAu2ybiIfkUXBXxaLMekN8X57PRz_zq1_hy9OMqd0KrPvdCeE-9XBSKB0YhOE-VUrKQXs6VMAY4K5hkLJjAIVDtMCitPfC5gmAMPyBf1-8uu_ZpwNjbuow-VeQabIdotRRSaMZpkqf_lSltAVrIBE_ewId26Jr0C8uoYiBTrxP6tka-a2PsMNhlV9auW1kK9mUadjuNZI83Dw7zGhevctP-BPI1KGOPz9u46x6t0lxLO7uZ2j-3fDK9nY6tSv7L2jsfX4t7n_gv5hSc9g</recordid><startdate>20081119</startdate><enddate>20081119</enddate><creator>Sarkar, Debanjan</creator><creator>Vemula, Praveen Kumar</creator><creator>Teo, Grace S. 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| ispartof | Bioconjugate chemistry, 2008-11, Vol.19 (11), p.2105-2109 |
| issn | 1043-1802 1520-4812 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Biotin - metabolism Carbohydrates Cell Differentiation Cell Survival Chemical engineering Fluorescence Genotype & phenotype Humans Leukocyte Rolling - physiology Leukocytes Mesenchymal Stromal Cells - chemistry Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Oligosaccharides - chemistry P-Selectin - metabolism Sensitivity and Specificity Staining and Labeling Stem cells Streptavidin - metabolism Tissues |
| title | Chemical Engineering of Mesenchymal Stem Cells to Induce a Cell Rolling Response |
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