Characterization of Nucleic Acids by Nanopore Analysis

Single-stranded DNA and RNA molecules in solution can be driven through a nanoscopic pore by an applied electric field. As each molecule occupies the pore, a characteristic blockade of ionic current is produced. Information about length, composition, structure, and dynamic motion of the molecule can...

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Veröffentlicht in:	Accounts of chemical research 2002-10, Vol.35 (10), p.817-825
Hauptverfasser:	Deamer, David W, Branton, Daniel
Format:	Artikel
Sprache:	eng
Schlagworte:	Biopolymers - chemistry DNA, Single-Stranded - chemistry Electric Conductivity Electrochemistry - methods Hemolysin Proteins - chemistry Nanotechnology - methods Nucleic Acid Conformation Nucleic Acids - chemistry Temperature
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Single-stranded DNA and RNA molecules in solution can be driven through a nanoscopic pore by an applied electric field. As each molecule occupies the pore, a characteristic blockade of ionic current is produced. Information about length, composition, structure, and dynamic motion of the molecule can be deduced from modulations of the current blockade.
ISSN:	0001-4842 1520-4898
DOI:	10.1021/ar000138m