Near-IR Absorption and Fluorescence Spectra and AFM Observation of the Light-Harvesting 1 Complex on a Mica Substrate Refolded from the Subunit Light-Harvesting 1 Complexes of Photosynthetic Bacteria Rhodospirillum rubrum
The subunit light-harvesting 1 (LH 1) complexes isolated from photosynthetic bacteria Rhodospirillum rubrum using n-octyl-β-glucoside were reassociated and adsorbed on a mica substrate using spin-coat methods with the aim of using this LH complex in a nanodevice. The near-IR absorption and fluoresce...
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| Veröffentlicht in: | Langmuir 2005-03, Vol.21 (7), p.3069-3075 |
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| creator | Iida, Kouji Inagaki, Jun-ichi Shinohara, Kiyoshi Suemori, Yoshiharu Ogawa, Makiko Dewa, Takehisa Nango, Mamoru |
| description | The subunit light-harvesting 1 (LH 1) complexes isolated from photosynthetic bacteria Rhodospirillum rubrum using n-octyl-β-glucoside were reassociated and adsorbed on a mica substrate using spin-coat methods with the aim of using this LH complex in a nanodevice. The near-IR absorption and fluorescence spectra of the LH 1 complexes indicated that the LH 1 complex on the mica was stable, and efficient energy transfer from a carotenoid to a bacteriochlorophyll a was observed. Atomic force microscopy of the reassociated LH 1 complexes, under air, showed the expected ringlike structure. The outer and inner diameters of the ringlike structure of the LH 1 complex were approximately 30 and 8 nm, respectively, and the ringlike structure protruded by 0.2−0.6 nm. |
| doi_str_mv | 10.1021/la047460g |
| format | Article |
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The near-IR absorption and fluorescence spectra of the LH 1 complexes indicated that the LH 1 complex on the mica was stable, and efficient energy transfer from a carotenoid to a bacteriochlorophyll a was observed. Atomic force microscopy of the reassociated LH 1 complexes, under air, showed the expected ringlike structure. 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The near-IR absorption and fluorescence spectra of the LH 1 complexes indicated that the LH 1 complex on the mica was stable, and efficient energy transfer from a carotenoid to a bacteriochlorophyll a was observed. Atomic force microscopy of the reassociated LH 1 complexes, under air, showed the expected ringlike structure. The outer and inner diameters of the ringlike structure of the LH 1 complex were approximately 30 and 8 nm, respectively, and the ringlike structure protruded by 0.2−0.6 nm.</description><subject>Aluminum Silicates</subject><subject>Amino Acid Sequence</subject><subject>Carotenoids</subject><subject>Chemistry</subject><subject>Conserved Sequence</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Light-Harvesting Protein Complexes - chemistry</subject><subject>Light-Harvesting Protein Complexes - metabolism</subject><subject>Light-Harvesting Protein Complexes - ultrastructure</subject><subject>Micelles</subject><subject>Microscopy, Atomic Force</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Photosynthesis</subject><subject>Photosystem I Protein Complex - chemistry</subject><subject>Photosystem I Protein Complex - metabolism</subject><subject>Photosystem I Protein Complex - ultrastructure</subject><subject>Protein Binding</subject><subject>Protein Denaturation</subject><subject>Protein Structure, Quaternary</subject><subject>Protein Subunits - chemistry</subject><subject>Protein Subunits - metabolism</subject><subject>Rhodospirillum rubrum</subject><subject>Rhodospirillum rubrum - enzymology</subject><subject>Rhodospirillum rubrum - ultrastructure</subject><subject>Sequence Alignment</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Infrared</subject><subject>Substrate Specificity</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v0zAYhyMEYmVw4AsgXwDtELATx46PXUW3Sd0f2nGO3jhO65HEme1M24fdd8Fpq_WCJh98-D1-_NP7RtFngn8QnJCfDWDKKcPrN9GEZAmOszzhb6MJ5jSNQ5AeRR-cu8MYi5SK99ERyTgXImeT6PlKgY0vlmhaOmN7r02HoKvQvBmMVU6qTiq06pX0FrbBdH6Jrkun7ANsYVMjv1FoodcbH5-DfVDO626NCJqZtm_UIxqN6FJLQKuhdMHjFVqq2jSVqlBtTbsVhGzotH9FpNz42c3GeOOeuvDGa4lOQXplNaDlxlTG9drqphlaZIfSDu3H6F0NjVOf9vdx9Gf-63Z2Hi-uzy5m00UMlAsfM5xgwERxkTECiomSEgICqzxLkoxiKTmjVU1qkCWUNaRQU1aWIpMVz1kq0uPo-87bW3M_hOJFq8PsmgY6ZQZX8IyGk-KR_PYqyXiWjnUCeLIDpTXOWVUXvdUt2KeC4GLcevGy9cB-2UuHslXVgdyvOQBf9wA4CU1toZPaHTjGRJ6T8dN4x2nn1eNLDvZvKJbyrLi9WRVnnNDf-emsuDp4Qbrizgy2C1P-T8F_NznTLA</recordid><startdate>20050329</startdate><enddate>20050329</enddate><creator>Iida, Kouji</creator><creator>Inagaki, Jun-ichi</creator><creator>Shinohara, Kiyoshi</creator><creator>Suemori, Yoshiharu</creator><creator>Ogawa, Makiko</creator><creator>Dewa, Takehisa</creator><creator>Nango, Mamoru</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050329</creationdate><title>Near-IR Absorption and Fluorescence Spectra and AFM Observation of the Light-Harvesting 1 Complex on a Mica Substrate Refolded from the Subunit Light-Harvesting 1 Complexes of Photosynthetic Bacteria Rhodospirillum rubrum</title><author>Iida, Kouji ; Inagaki, Jun-ichi ; Shinohara, Kiyoshi ; Suemori, Yoshiharu ; Ogawa, Makiko ; Dewa, Takehisa ; Nango, Mamoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a479t-6020a01e79561ae69b411a90e8522540cc764df1facbabfa3af46bb95cd786393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aluminum Silicates</topic><topic>Amino Acid Sequence</topic><topic>Carotenoids</topic><topic>Chemistry</topic><topic>Conserved Sequence</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Light-Harvesting Protein Complexes - chemistry</topic><topic>Light-Harvesting Protein Complexes - metabolism</topic><topic>Light-Harvesting Protein Complexes - ultrastructure</topic><topic>Micelles</topic><topic>Microscopy, Atomic Force</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Photosynthesis</topic><topic>Photosystem I Protein Complex - chemistry</topic><topic>Photosystem I Protein Complex - metabolism</topic><topic>Photosystem I Protein Complex - ultrastructure</topic><topic>Protein Binding</topic><topic>Protein Denaturation</topic><topic>Protein Structure, Quaternary</topic><topic>Protein Subunits - chemistry</topic><topic>Protein Subunits - metabolism</topic><topic>Rhodospirillum rubrum</topic><topic>Rhodospirillum rubrum - enzymology</topic><topic>Rhodospirillum rubrum - ultrastructure</topic><topic>Sequence Alignment</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Infrared</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iida, Kouji</creatorcontrib><creatorcontrib>Inagaki, Jun-ichi</creatorcontrib><creatorcontrib>Shinohara, Kiyoshi</creatorcontrib><creatorcontrib>Suemori, Yoshiharu</creatorcontrib><creatorcontrib>Ogawa, Makiko</creatorcontrib><creatorcontrib>Dewa, Takehisa</creatorcontrib><creatorcontrib>Nango, Mamoru</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iida, Kouji</au><au>Inagaki, Jun-ichi</au><au>Shinohara, Kiyoshi</au><au>Suemori, Yoshiharu</au><au>Ogawa, Makiko</au><au>Dewa, Takehisa</au><au>Nango, Mamoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Near-IR Absorption and Fluorescence Spectra and AFM Observation of the Light-Harvesting 1 Complex on a Mica Substrate Refolded from the Subunit Light-Harvesting 1 Complexes of Photosynthetic Bacteria Rhodospirillum rubrum</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2005-03-29</date><risdate>2005</risdate><volume>21</volume><issue>7</issue><spage>3069</spage><epage>3075</epage><pages>3069-3075</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>The subunit light-harvesting 1 (LH 1) complexes isolated from photosynthetic bacteria Rhodospirillum rubrum using n-octyl-β-glucoside were reassociated and adsorbed on a mica substrate using spin-coat methods with the aim of using this LH complex in a nanodevice. The near-IR absorption and fluorescence spectra of the LH 1 complexes indicated that the LH 1 complex on the mica was stable, and efficient energy transfer from a carotenoid to a bacteriochlorophyll a was observed. Atomic force microscopy of the reassociated LH 1 complexes, under air, showed the expected ringlike structure. The outer and inner diameters of the ringlike structure of the LH 1 complex were approximately 30 and 8 nm, respectively, and the ringlike structure protruded by 0.2−0.6 nm.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15779986</pmid><doi>10.1021/la047460g</doi><tpages>7</tpages></addata></record> |
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| subjects | Aluminum Silicates Amino Acid Sequence Carotenoids Chemistry Conserved Sequence Exact sciences and technology General and physical chemistry Light-Harvesting Protein Complexes - chemistry Light-Harvesting Protein Complexes - metabolism Light-Harvesting Protein Complexes - ultrastructure Micelles Microscopy, Atomic Force Models, Molecular Molecular Sequence Data Photosynthesis Photosystem I Protein Complex - chemistry Photosystem I Protein Complex - metabolism Photosystem I Protein Complex - ultrastructure Protein Binding Protein Denaturation Protein Structure, Quaternary Protein Subunits - chemistry Protein Subunits - metabolism Rhodospirillum rubrum Rhodospirillum rubrum - enzymology Rhodospirillum rubrum - ultrastructure Sequence Alignment Spectrometry, Fluorescence Spectrophotometry, Infrared Substrate Specificity |
| title | Near-IR Absorption and Fluorescence Spectra and AFM Observation of the Light-Harvesting 1 Complex on a Mica Substrate Refolded from the Subunit Light-Harvesting 1 Complexes of Photosynthetic Bacteria Rhodospirillum rubrum |
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