Exploitation of the Coil−Globule Plasmid DNA Transition Induced by Small Changes in Temperature, pH Salt, and Poly(ethylene glycol) Compositions for Directed Partitioning in Aqueous Two-Phase Systems

In this study, the interplay of two linked equilibria is examined, one concerning an aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and salt employed to partition plasmid DNA (pDNA), and the other a potential structural transition of pDNA depending on PEG and salt concentrat...

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Veröffentlicht in:Langmuir 2006-04, Vol.22 (9), p.4282-4290
Hauptverfasser: Frerix, Andreas, Schönewald, Michael, Geilenkirchen, Petra, Müller, Markus, Kula, Maria-Regina, Hubbuch, Jürgen
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container_end_page 4290
container_issue 9
container_start_page 4282
container_title Langmuir
container_volume 22
creator Frerix, Andreas
Schönewald, Michael
Geilenkirchen, Petra
Müller, Markus
Kula, Maria-Regina
Hubbuch, Jürgen
description In this study, the interplay of two linked equilibria is examined, one concerning an aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and salt employed to partition plasmid DNA (pDNA), and the other a potential structural transition of pDNA depending on PEG and salt concentration and other system parameters. The boundary conditions for pDNA partitioning are set by PEG and salt concentrations, PEG molecular weight, pH, and temperature. While investigating these parameters, it was found that a small increase/decrease of the respective values led to a drastic and significant change in pDNA behavior. This behavior could be attributed to a coil−globule transition of the pDNA triggered by the respective phase conditions. The combination of this structural change, aggregation effects linked to the transition process, and the electrostatic potential difference found in PEG−salt systems thus offers a sensitive way to separate nucleic acid forms on the basis of their unique property to undergo coil−globule transitions under distinct system properties.
doi_str_mv 10.1021/la052745u
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subjects Chemistry
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
Exact sciences and technology
General and physical chemistry
Hydrogen-Ion Concentration
In Vitro Techniques
Nucleic Acid Conformation
Plasmids - chemistry
Plasmids - isolation & purification
Polyethylene Glycols
RNA, Bacterial - chemistry
RNA, Bacterial - isolation & purification
Sodium Chloride
Solubility
Temperature
Water
title Exploitation of the Coil−Globule Plasmid DNA Transition Induced by Small Changes in Temperature, pH Salt, and Poly(ethylene glycol) Compositions for Directed Partitioning in Aqueous Two-Phase Systems
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