Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer
Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1−5) were selected as...
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| Veröffentlicht in: | Environmental science & technology 2009-12, Vol.43 (24), p.9335-9340 |
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| creator | Kim, Mina Um, Hyun-Ju Bang, SunBaek Lee, Sang-Hee Oh, Suk-Jung Han, Ji-Hye Kim, Kyoung-Woong Min, Jiho Kim, Yang-Hoon |
| description | Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1−5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K d) of 4.95 ± 0.31 and 7.05 ± 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 μg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3. |
| doi_str_mv | 10.1021/es902407g |
| format | Article |
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On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1−5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K d) of 4.95 ± 0.31 and 7.05 ± 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 μg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es902407g</identifier><identifier>PMID: 20000526</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal, plant and microbial ecology ; Applied ecology ; Applied sciences ; Aptamers, Nucleotide - chemistry ; Arsenic ; Arsenic - chemistry ; Arsenic removal ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; Ecotoxicology, biological effects of pollution ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Groundwater ; Humans ; Molecular Structure ; Nucleic Acid Conformation ; Pollution ; Remediation and Control Technologies ; Resins ; Rivers ; Rivers - chemistry ; SELEX Aptamer Technique ; Surface Plasmon Resonance - methods ; Vietnam ; Water Pollutants, Chemical - chemistry ; Water Purification - instrumentation ; Water Purification - methods ; Water Supply - analysis</subject><ispartof>Environmental science & technology, 2009-12, Vol.43 (24), p.9335-9340</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Dec 15, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a403t-af5332976bfe6a85d74c2e17540d5c3e8fa8186550100ebd86e705130f5362423</citedby><cites>FETCH-LOGICAL-a403t-af5332976bfe6a85d74c2e17540d5c3e8fa8186550100ebd86e705130f5362423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es902407g$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es902407g$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22236094$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20000526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Mina</creatorcontrib><creatorcontrib>Um, Hyun-Ju</creatorcontrib><creatorcontrib>Bang, SunBaek</creatorcontrib><creatorcontrib>Lee, Sang-Hee</creatorcontrib><creatorcontrib>Oh, Suk-Jung</creatorcontrib><creatorcontrib>Han, Ji-Hye</creatorcontrib><creatorcontrib>Kim, Kyoung-Woong</creatorcontrib><creatorcontrib>Min, Jiho</creatorcontrib><creatorcontrib>Kim, Yang-Hoon</creatorcontrib><title>Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1−5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K d) of 4.95 ± 0.31 and 7.05 ± 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 μg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.</description><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Applied sciences</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Arsenic</subject><subject>Arsenic - chemistry</subject><subject>Arsenic removal</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Groundwater</subject><subject>Humans</subject><subject>Molecular Structure</subject><subject>Nucleic Acid Conformation</subject><subject>Pollution</subject><subject>Remediation and Control Technologies</subject><subject>Resins</subject><subject>Rivers</subject><subject>Rivers - chemistry</subject><subject>SELEX Aptamer Technique</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Vietnam</subject><subject>Water Pollutants, Chemical - chemistry</subject><subject>Water Purification - instrumentation</subject><subject>Water Purification - methods</subject><subject>Water Supply - analysis</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0V1LwzAUBuAgipvTC_-AFEHEi-rJZ9vLOXUKQ1GceFey9nR29GMmreK_N2NzA73xKiE878lJDiGHFM4pMHqBNgImIJhukS6VDHwZSrpNugCU-xFXrx2yZ-0MABiHcJd0mNuCZKpLHvvGYpUn3hOW9YcuvMzUpfeSY1PpEi16Q1O3VfqpGzTe2ObV1Gve0Ful_Mu8ShdnV_d9rz9vXMTsk51MFxYPVmuPjG-unwe3_uhheDfoj3wtgDe-ziTnLArUJEOlQ5kGImFIAykglQnHMNMhDZWUQAFwkoYKA5CUg8spJhjvkdNl3bmp31u0TVzmNsGi0BXWrY1dJSl4qP4huWBSCSGdPP4lZ3VrKveM2P0cFaB45NDZEiWmttZgFs9NXmrzFVOIF_OI1_Nw9mhVsJ2UmK7lzwAcOFkBbRNdZEZXSW43jjGuIBIbpxO7aervhd--UJsV</recordid><startdate>20091215</startdate><enddate>20091215</enddate><creator>Kim, Mina</creator><creator>Um, Hyun-Ju</creator><creator>Bang, SunBaek</creator><creator>Lee, Sang-Hee</creator><creator>Oh, Suk-Jung</creator><creator>Han, Ji-Hye</creator><creator>Kim, Kyoung-Woong</creator><creator>Min, Jiho</creator><creator>Kim, Yang-Hoon</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7QH</scope><scope>7TM</scope><scope>7TV</scope><scope>7UA</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20091215</creationdate><title>Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer</title><author>Kim, Mina ; Um, Hyun-Ju ; Bang, SunBaek ; Lee, Sang-Hee ; Oh, Suk-Jung ; Han, Ji-Hye ; Kim, Kyoung-Woong ; Min, Jiho ; Kim, Yang-Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a403t-af5332976bfe6a85d74c2e17540d5c3e8fa8186550100ebd86e705130f5362423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>Applied sciences</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Arsenic</topic><topic>Arsenic - chemistry</topic><topic>Arsenic removal</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Groundwater</topic><topic>Humans</topic><topic>Molecular Structure</topic><topic>Nucleic Acid Conformation</topic><topic>Pollution</topic><topic>Remediation and Control Technologies</topic><topic>Resins</topic><topic>Rivers</topic><topic>Rivers - chemistry</topic><topic>SELEX Aptamer Technique</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Vietnam</topic><topic>Water Pollutants, Chemical - chemistry</topic><topic>Water Purification - instrumentation</topic><topic>Water Purification - methods</topic><topic>Water Supply - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Mina</creatorcontrib><creatorcontrib>Um, Hyun-Ju</creatorcontrib><creatorcontrib>Bang, SunBaek</creatorcontrib><creatorcontrib>Lee, Sang-Hee</creatorcontrib><creatorcontrib>Oh, Suk-Jung</creatorcontrib><creatorcontrib>Han, Ji-Hye</creatorcontrib><creatorcontrib>Kim, Kyoung-Woong</creatorcontrib><creatorcontrib>Min, Jiho</creatorcontrib><creatorcontrib>Kim, Yang-Hoon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Nucleic Acids Abstracts</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Mina</au><au>Um, Hyun-Ju</au><au>Bang, SunBaek</au><au>Lee, Sang-Hee</au><au>Oh, Suk-Jung</au><au>Han, Ji-Hye</au><au>Kim, Kyoung-Woong</au><au>Min, Jiho</au><au>Kim, Yang-Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2009-12-15</date><risdate>2009</risdate><volume>43</volume><issue>24</issue><spage>9335</spage><epage>9340</epage><pages>9335-9340</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1−5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K d) of 4.95 ± 0.31 and 7.05 ± 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 μg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20000526</pmid><doi>10.1021/es902407g</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal, plant and microbial ecology Applied ecology Applied sciences Aptamers, Nucleotide - chemistry Arsenic Arsenic - chemistry Arsenic removal Biological and medical sciences Deoxyribonucleic acid DNA Ecotoxicology, biological effects of pollution Exact sciences and technology Fundamental and applied biological sciences. Psychology Groundwater Humans Molecular Structure Nucleic Acid Conformation Pollution Remediation and Control Technologies Resins Rivers Rivers - chemistry SELEX Aptamer Technique Surface Plasmon Resonance - methods Vietnam Water Pollutants, Chemical - chemistry Water Purification - instrumentation Water Purification - methods Water Supply - analysis |
| title | Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer |
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