Electron Paramagnetic Resonance Study of Radiation Damage in Photosynthetic Reaction Center Crystals

Electron paramagnetic resonance (EPR) was used to simultaneously study radiation-induced cofactor reduction and damaging radical formation in single crystals of the bacterial reaction center (RC). Crystals of Fe-removed/Zn-replaced RC protein from Rhodobacter (R.) sphaeroides R26 were irradiated wit...

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Veröffentlicht in:	Biochemistry 2008-09, Vol.47 (35), p.9251-9257
Hauptverfasser:	Utschig, Lisa M, Chemerisov, Sergey D, Tiede, David M, Poluektov, Oleg G
Format:	Artikel
Sprache:	eng
Schlagworte:	BASIC BIOLOGICAL SCIENCES BENZOQUINONES CRYOGENICS DIFFRACTION ELECTRON SPIN RESONANCE Electron Spin Resonance Spectroscopy - methods ELECTRON TRANSFER Free Radicals GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE Iron - chemistry Models, Molecular MONOCRYSTALS Photosynthetic Reaction Center Complex Proteins - chemistry Photosynthetic Reaction Center Complex Proteins - radiation effects PHOTOSYNTHETIC REACTION CENTERS Protein Conformation PROTEINS QUINONES RADIATION DOSES RADIATIONS RADICALS Rhodobacter Rhodobacter sphaeroides - metabolism SATURATION SPECTRA X-RAY DIFFRACTION Zinc - chemistry
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