Residue 752 in DNA polymerase of equine herpesvirus type 1 is non-essential for virus growth in vitro

A single amino acid variation in the equine herpesvirus type 1 (EHV-1) DNA polymerase (Pol) (D752/N752) determines its neuropathogenic potential. Here, an EHV-1 strain RacL11 mutant with a deletion of Pol residue 752 was constructed. The deletion virus was then repaired to encode D752 or N752, respe...

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Veröffentlicht in:Journal of general virology 2010-07, Vol.91 (Pt 7), p.1817-1822
Hauptverfasser: Ma, Guanggang, Lu, Chengping, Osterrieder, Nikolaus
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creator Ma, Guanggang
Lu, Chengping
Osterrieder, Nikolaus
description A single amino acid variation in the equine herpesvirus type 1 (EHV-1) DNA polymerase (Pol) (D752/N752) determines its neuropathogenic potential. Here, an EHV-1 strain RacL11 mutant with a deletion of Pol residue 752 was constructed. The deletion virus was then repaired to encode D752 or N752, respectively. The Delta752 mutant virus replicated with kinetics indistinguishable from those of D752 and N752 viruses. In addition, we could demonstrate that the deletion mutant was significantly more resistant to aphidicolin, a drug targeting Pol, compared with the N752 but not the D752 variant. In equine peripheral blood mononuclear cells, no significant difference was detected between the mutants with respect to cellular tropism or virus replication. The results demonstrated that amino acid residue 752 in EHV-1 Pol is not required for virus growth, and that only the N752 mutation confers a drug-sensitive phenotype to the virus.
doi_str_mv 10.1099/vir.0.018036-0
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subjects Amino Acid Sequence
Animals
Cells, Cultured
DNA-Directed DNA Polymerase - chemistry
DNA-Directed DNA Polymerase - genetics
DNA-Directed DNA Polymerase - metabolism
Equine herpesvirus
Gene Deletion
Herpesvirus 1, Equid - genetics
Herpesvirus 1, Equid - physiology
Horses
Leukocytes, Mononuclear - virology
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Viral Tropism - genetics
Virus Replication
title Residue 752 in DNA polymerase of equine herpesvirus type 1 is non-essential for virus growth in vitro
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