Selection and Characterization of Cellulose-Deficient Derivates of Shiga Toxin-Producing Escherichia coli
Shiga toxin-producing Escherichia coli (STEC) is known to have several defense mechanisms, one of which is the production of extracellular substances including cellulose. The goal of this study was to prepare pairs of STEC cultures for use in future studies designed to address the role of cellulose...
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| Veröffentlicht in: | Journal of food protection 2010-06, Vol.73 (6), p.1038-1046 |
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| creator | BYONG KWON YOO STEWART, Tod GUARD-BOULDIN, Jean MUSGROVE, Michael GAST, Richard JINRU CHEN |
| description | Shiga toxin-producing Escherichia coli (STEC) is known to have several defense mechanisms, one of which is the production of extracellular substances including cellulose. The goal of this study was to prepare pairs of STEC cultures for use in future studies designed to address the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions. Cells of STEC deficient in cellulose production were separated from cellulose-proficient wild-type cells. The identities of the two types of cells were confirmed using serotyping and pulsed-field gel electrophoresis (PFGE). Selected growth characteristics of the two types of cells were determined using three phenotype microarray plates, PM9, PM10, and PM11. The cellulose-deficient and cellulose-proficient cells in each STEC pair shared the same serotype and PFGE profile. The deficiency in cellulose production did not significantly (P > 0.05) affect the growth characteristics of STEC cells under 191 of the 210 tested growth conditions. Significant differences in growth between the two types of cells were observed only in the presence of two antibiotics, a short chain fatty acid, and high concentrations of osmolytes, as well as under extreme acidic and alkaline pH. These results suggest that deficiency in cellulose production did not alter the serological property, PFGE profile, and growth characteristics of selected STEC strains under optimal growth conditions. The STEC strains and their cellulose-deficient derivates could be useful for studying the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions. |
| doi_str_mv | 10.4315/0362-028X-73.6.1038 |
| format | Article |
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The goal of this study was to prepare pairs of STEC cultures for use in future studies designed to address the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions. Cells of STEC deficient in cellulose production were separated from cellulose-proficient wild-type cells. The identities of the two types of cells were confirmed using serotyping and pulsed-field gel electrophoresis (PFGE). Selected growth characteristics of the two types of cells were determined using three phenotype microarray plates, PM9, PM10, and PM11. The cellulose-deficient and cellulose-proficient cells in each STEC pair shared the same serotype and PFGE profile. The deficiency in cellulose production did not significantly (P > 0.05) affect the growth characteristics of STEC cells under 191 of the 210 tested growth conditions. Significant differences in growth between the two types of cells were observed only in the presence of two antibiotics, a short chain fatty acid, and high concentrations of osmolytes, as well as under extreme acidic and alkaline pH. These results suggest that deficiency in cellulose production did not alter the serological property, PFGE profile, and growth characteristics of selected STEC strains under optimal growth conditions. The STEC strains and their cellulose-deficient derivates could be useful for studying the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-73.6.1038</identifier><identifier>PMID: 20537258</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association for Food Protection</publisher><subject>Antibiotics ; Biofilms ; Biological and medical sciences ; Cellulose ; Cellulose - biosynthesis ; Colony Count, Microbial - methods ; Defense mechanisms ; E coli ; Electrophoresis, Gel, Pulsed-Field ; Environmental conditions ; Epoxy resins ; Escherichia coli ; Ethanol ; Food contamination & poisoning ; Food industries ; Food Microbiology ; Food safety ; Food science ; Fundamental and applied biological sciences. Psychology ; Glycerol ; Growth conditions ; Microbial Viability ; Morphology ; Particulate matter ; Pathogens ; Phylogeny ; Shiga-Toxigenic Escherichia coli - classification ; Shiga-Toxigenic Escherichia coli - growth & development ; Shiga-Toxigenic Escherichia coli - metabolism ; Species Specificity ; Toxins</subject><ispartof>Journal of food protection, 2010-06, Vol.73 (6), p.1038-1046</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright International Association for Food Protection Jun 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-ee059bb314cc0f4331578acdd099fe4485836b5890c7aa521c8aa008a42da98d3</citedby><cites>FETCH-LOGICAL-c438t-ee059bb314cc0f4331578acdd099fe4485836b5890c7aa521c8aa008a42da98d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/366113167?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22852947$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20537258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BYONG KWON YOO</creatorcontrib><creatorcontrib>STEWART, Tod</creatorcontrib><creatorcontrib>GUARD-BOULDIN, Jean</creatorcontrib><creatorcontrib>MUSGROVE, Michael</creatorcontrib><creatorcontrib>GAST, Richard</creatorcontrib><creatorcontrib>JINRU CHEN</creatorcontrib><title>Selection and Characterization of Cellulose-Deficient Derivates of Shiga Toxin-Producing Escherichia coli</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>Shiga toxin-producing Escherichia coli (STEC) is known to have several defense mechanisms, one of which is the production of extracellular substances including cellulose. The goal of this study was to prepare pairs of STEC cultures for use in future studies designed to address the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions. Cells of STEC deficient in cellulose production were separated from cellulose-proficient wild-type cells. The identities of the two types of cells were confirmed using serotyping and pulsed-field gel electrophoresis (PFGE). Selected growth characteristics of the two types of cells were determined using three phenotype microarray plates, PM9, PM10, and PM11. The cellulose-deficient and cellulose-proficient cells in each STEC pair shared the same serotype and PFGE profile. The deficiency in cellulose production did not significantly (P > 0.05) affect the growth characteristics of STEC cells under 191 of the 210 tested growth conditions. Significant differences in growth between the two types of cells were observed only in the presence of two antibiotics, a short chain fatty acid, and high concentrations of osmolytes, as well as under extreme acidic and alkaline pH. These results suggest that deficiency in cellulose production did not alter the serological property, PFGE profile, and growth characteristics of selected STEC strains under optimal growth conditions. The STEC strains and their cellulose-deficient derivates could be useful for studying the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions.</description><subject>Antibiotics</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Cellulose</subject><subject>Cellulose - biosynthesis</subject><subject>Colony Count, Microbial - methods</subject><subject>Defense mechanisms</subject><subject>E coli</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Environmental conditions</subject><subject>Epoxy resins</subject><subject>Escherichia coli</subject><subject>Ethanol</subject><subject>Food contamination & poisoning</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Food safety</subject><subject>Food science</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol</subject><subject>Growth conditions</subject><subject>Microbial Viability</subject><subject>Morphology</subject><subject>Particulate matter</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Shiga-Toxigenic Escherichia coli - classification</subject><subject>Shiga-Toxigenic Escherichia coli - growth & development</subject><subject>Shiga-Toxigenic Escherichia coli - metabolism</subject><subject>Species Specificity</subject><subject>Toxins</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0d9LHDEQB_BQKnpa_4JCWQqlT3vNz03yWE5rBUFBBd_C3GzWi-xtbLJb2v71ZutVoU-B4TND5juEvGd0KQVTX6hoeE25uau1WDZLRoV5QxbMSllbavVbsngRB-Qw5wdKKbe82ScHnCqhuTILEq5973EMcahgaKvVBhLg6FP4A3-LsatWvu-nPmZfn_guYPDDWJ0U8RNGn2dwvQn3UN3EX2Gor1JsJwzDfXWacVMUbgJUGPvwjux10Gd_vHuPyO2305vV9_ri8ux89fWiRinMWHtPlV2vBZOItJOibKoNYNtSazsvpVFGNGtlLEUNoDhDA0CpAclbsKYVR-Tz89zHFH9MPo9uGzKWHWDwccpOK6mEZJwX-fE_-RCnNJTPOdE0jAnW6ILEM8IUc06-c48pbCH9doy6-Q5uTtnNKTstXOPmO5SuD7vR03rr25eef8EX8GkHICP0XYIBQ3513ChupRZPmoWQaQ</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>BYONG KWON YOO</creator><creator>STEWART, Tod</creator><creator>GUARD-BOULDIN, Jean</creator><creator>MUSGROVE, Michael</creator><creator>GAST, Richard</creator><creator>JINRU CHEN</creator><general>International Association for Food Protection</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7WY</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>883</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0F</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20100601</creationdate><title>Selection and Characterization of Cellulose-Deficient Derivates of Shiga Toxin-Producing Escherichia coli</title><author>BYONG KWON YOO ; STEWART, Tod ; GUARD-BOULDIN, Jean ; MUSGROVE, Michael ; GAST, Richard ; JINRU CHEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-ee059bb314cc0f4331578acdd099fe4485836b5890c7aa521c8aa008a42da98d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antibiotics</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Cellulose</topic><topic>Cellulose - biosynthesis</topic><topic>Colony Count, Microbial - methods</topic><topic>Defense mechanisms</topic><topic>E coli</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Environmental conditions</topic><topic>Epoxy resins</topic><topic>Escherichia coli</topic><topic>Ethanol</topic><topic>Food contamination & poisoning</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Food safety</topic><topic>Food science</topic><topic>Fundamental and applied biological sciences. 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The goal of this study was to prepare pairs of STEC cultures for use in future studies designed to address the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions. Cells of STEC deficient in cellulose production were separated from cellulose-proficient wild-type cells. The identities of the two types of cells were confirmed using serotyping and pulsed-field gel electrophoresis (PFGE). Selected growth characteristics of the two types of cells were determined using three phenotype microarray plates, PM9, PM10, and PM11. The cellulose-deficient and cellulose-proficient cells in each STEC pair shared the same serotype and PFGE profile. The deficiency in cellulose production did not significantly (P > 0.05) affect the growth characteristics of STEC cells under 191 of the 210 tested growth conditions. Significant differences in growth between the two types of cells were observed only in the presence of two antibiotics, a short chain fatty acid, and high concentrations of osmolytes, as well as under extreme acidic and alkaline pH. These results suggest that deficiency in cellulose production did not alter the serological property, PFGE profile, and growth characteristics of selected STEC strains under optimal growth conditions. The STEC strains and their cellulose-deficient derivates could be useful for studying the role of cellulose in protecting the cells of STEC for survival under adverse environmental conditions.</abstract><cop>Des Moines, IA</cop><pub>International Association for Food Protection</pub><pmid>20537258</pmid><doi>10.4315/0362-028X-73.6.1038</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Biofilms Biological and medical sciences Cellulose Cellulose - biosynthesis Colony Count, Microbial - methods Defense mechanisms E coli Electrophoresis, Gel, Pulsed-Field Environmental conditions Epoxy resins Escherichia coli Ethanol Food contamination & poisoning Food industries Food Microbiology Food safety Food science Fundamental and applied biological sciences. Psychology Glycerol Growth conditions Microbial Viability Morphology Particulate matter Pathogens Phylogeny Shiga-Toxigenic Escherichia coli - classification Shiga-Toxigenic Escherichia coli - growth & development Shiga-Toxigenic Escherichia coli - metabolism Species Specificity Toxins |
| title | Selection and Characterization of Cellulose-Deficient Derivates of Shiga Toxin-Producing Escherichia coli |
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