Localization of photosynthetic membrane components in Rhodopseudomonas sphaeroides by a radioactive labeling procedure

Reduction with [3H]KBH4 of Schiff's bases generated by reaction with pyridoxal 5'-phosphate (which cannot penetrate the intact cytoplasmic membrane) yields tritium-labeled derivatives of both proteins and lipids accessible on the periplasmic side of the cytoplasmic membrane. Application of...

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Veröffentlicht in:	Biochemistry (Easton) 1980-10, Vol.19 (22), p.5104-5111
Hauptverfasser:	Francis, Gordon A, Richards, William R
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques 550301 - Cytology- Tracer Techniques BACTERIA Bacterial Chromatophores - analysis BASIC BIOLOGICAL SCIENCES BIOCHEMISTRY Borohydrides CELL CONSTITUENTS Cell Fractionation Cell Membrane - analysis CELL MEMBRANES CENTRIFUGATION Centrifugation, Density Gradient CHEMICAL ANALYSIS CHEMICAL REACTIONS CHEMISTRY ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel Isotope Labeling - methods LABELLED COMPOUNDS Membrane Proteins - analysis MEMBRANES MICROORGANISMS MORPHOLOGY ORGANIC COMPOUNDS PHOTOCHEMICAL REACTIONS PHOTOSYNTHESIS PROTEINS QUANTITATIVE CHEMICAL ANALYSIS Rhodobacter sphaeroides - analysis RHODOPSEUDOMONAS SEPARATION PROCESSES Space life sciences STRUCTURAL CHEMICAL ANALYSIS SYNTHESIS Tritium TRITIUM COMPOUNDS
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