Formation of Cell-Sized Single-Layered Liposomes in a Simple System of Phospholipid, Ethanol and Water
A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50°C for 30min, and then observed by a phasecontrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional...
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| Veröffentlicht in: | The Tohoku Journal of Experimental Medicine 1980, Vol.131(4), pp.399-407 |
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| container_end_page | 407 |
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| container_title | The Tohoku Journal of Experimental Medicine |
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| creator | GOTO, KUNIHIKO SATO, HARUO |
| description | A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50°C for 30min, and then observed by a phasecontrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4, stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. The nature of these liposomes was discussed in relation to the structure and function of the cell membrane. |
| doi_str_mv | 10.1620/tjem.131.399 |
| format | Article |
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Exp. Med.</addtitle><description>A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50°C for 30min, and then observed by a phasecontrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4, stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. The nature of these liposomes was discussed in relation to the structure and function of the cell membrane.</description><subject>cell-sized bilayer</subject><subject>Ethanol</subject><subject>lanthanum nitrate</subject><subject>liposome</subject><subject>Liposomes - chemical synthesis</subject><subject>Microscopy, Phase-Contrast</subject><subject>Phosphatidylcholines</subject><subject>semipermeability</subject><subject>Sonication</subject><subject>Water</subject><issn>0040-8727</issn><issn>1349-3329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM-LEzEYhoMoa3f15lXIydNOza9pkqOUrSsUFKp4DJnMl23KzGRM0kP9601t6SUf4X2-B74XoQ-ULOmKkc_lAOOScrrkWr9CC8qFbjhn-jVaECJIoySTb9F9zgdCuCBydYfuJCGKtWqB_Cam0ZYQJxw9XsMwNLvwF3q8C9PLAM3WniDV7zbMMccRMg4TtjUd5wHw7pQLjOfNH_uY530cwhz6R_xU9naKA7ZTj3_bAukdeuPtkOH9dT6gX5unn-vnZvv967f1l23jWqlLA8JaC9Iz4F5oBdI5ZlsPvaWqo10vHDDRdV0LvGMCtHdKKd3aFaXOrxTjD-jTxTun-OcIuZgxZFevshPEYzayFbyVVFTw8QK6FHNO4M2cwmjTyVBizrWac62m1mpqrRX_ePUeuxH6G3ztseabS37Ixb7ALbepBDfAfxnV1XoWiutbxTfA7W0yMPF_MKKNyQ</recordid><startdate>19800101</startdate><enddate>19800101</enddate><creator>GOTO, KUNIHIKO</creator><creator>SATO, HARUO</creator><general>Tohoku University Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800101</creationdate><title>Formation of Cell-Sized Single-Layered Liposomes in a Simple System of Phospholipid, Ethanol and Water</title><author>GOTO, KUNIHIKO ; SATO, HARUO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-e4aaae7f2e3f498e7cc2a5feda18b1bd4ce24bbb5e3b24e9fc88895a611cf6823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>cell-sized bilayer</topic><topic>Ethanol</topic><topic>lanthanum nitrate</topic><topic>liposome</topic><topic>Liposomes - chemical synthesis</topic><topic>Microscopy, Phase-Contrast</topic><topic>Phosphatidylcholines</topic><topic>semipermeability</topic><topic>Sonication</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GOTO, KUNIHIKO</creatorcontrib><creatorcontrib>SATO, HARUO</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Tohoku Journal of Experimental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GOTO, KUNIHIKO</au><au>SATO, HARUO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of Cell-Sized Single-Layered Liposomes in a Simple System of Phospholipid, Ethanol and Water</atitle><jtitle>The Tohoku Journal of Experimental Medicine</jtitle><addtitle>Tohoku J. Exp. Med.</addtitle><date>1980-01-01</date><risdate>1980</risdate><volume>131</volume><issue>4</issue><spage>399</spage><epage>407</epage><pages>399-407</pages><issn>0040-8727</issn><eissn>1349-3329</eissn><abstract>A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50°C for 30min, and then observed by a phasecontrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4, stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. 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| ispartof | The Tohoku Journal of Experimental Medicine, 1980, Vol.131(4), pp.399-407 |
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| language | eng |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | cell-sized bilayer Ethanol lanthanum nitrate liposome Liposomes - chemical synthesis Microscopy, Phase-Contrast Phosphatidylcholines semipermeability Sonication Water |
| title | Formation of Cell-Sized Single-Layered Liposomes in a Simple System of Phospholipid, Ethanol and Water |
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