Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats
The conjugation of 4-methylumbelliferone, p-nitrophenol and o-aminophenol in isolated rat liver cells was studied. It was found that for maximum sulphate conjugation to take place a very high sulphate concentration (50 mM) was needed, indicating that the rate limiting step may be the formation of ‘a...
Gespeichert in:
| Veröffentlicht in: | Biochemical pharmacology 1980-11, Vol.29 (21), p.2963-2968 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2968 |
|---|---|
| container_issue | 21 |
| container_start_page | 2963 |
| container_title | Biochemical pharmacology |
| container_volume | 29 |
| creator | Suolinna, Else-Maj Mäntylä, Eero |
| description | The conjugation of 4-methylumbelliferone,
p-nitrophenol and
o-aminophenol in isolated rat liver cells was studied. It was found that for maximum sulphate conjugation to take place a very high sulphate concentration (50 mM) was needed, indicating that the rate limiting step may be the formation of ‘active sulphate’. Under these conditions only a slight increase in the glucuronide/sulphate ratio with increasing substrate concentration was seen. Inducers of drug metabolism. PCB and phenobarbital, increased only glucuronidation. PCB was also found to cause qualitative changes in the metabolism of 4-methylumbelliferone. |
| doi_str_mv | 10.1016/0006-2952(80)90045-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75427944</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006295280900453</els_id><sourcerecordid>75427944</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-7dc606aad3f93c8aa12f2cb9d88525274b952986831b91348b42929e4d3e52723</originalsourceid><addsrcrecordid>eNp9kEtr3DAUhUVpSSdp_0EKWpVm4VYv29Km0A55FALNIl0LWbpOFDSWK8mB_vvKM0OWAYHQPefcq_shdE7JV0po940Q0jVMteyLJBeKENE2_A3aUNnzWu7kW7R5sbxHpzk_rU_Z0RN00vW9koxu0HwdFrukOHkH2EwO5yXMj6YAtnF6Wh5M8XHCvp4cQy07HPwzJGwhhIzHFHersaQY9un5EaY4mDT4YkKDY8J3259NSbCPJlPyB_RuNCHDx-N9hv5cXd5vb5rb39e_tj9uG8t6Vpre2Y50xjg-Km6lMZSNzA7KSdmylvViqFsp2UlOB0W5kINgiikQjkOVGT9Dnw995xT_LpCL3vm8_tpMEJes-1awXglRjeJgtCnmnGDUc_I7k_5pSvQKWq_Y9EpRS6L3oDWvsU_H_suwA_cSOpKt-veDDnXJZw9JZ-thsuB8Alu0i_71Af8B0vyNKQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75427944</pqid></control><display><type>article</type><title>Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Suolinna, Else-Maj ; Mäntylä, Eero</creator><creatorcontrib>Suolinna, Else-Maj ; Mäntylä, Eero</creatorcontrib><description>The conjugation of 4-methylumbelliferone,
p-nitrophenol and
o-aminophenol in isolated rat liver cells was studied. It was found that for maximum sulphate conjugation to take place a very high sulphate concentration (50 mM) was needed, indicating that the rate limiting step may be the formation of ‘active sulphate’. Under these conditions only a slight increase in the glucuronide/sulphate ratio with increasing substrate concentration was seen. Inducers of drug metabolism. PCB and phenobarbital, increased only glucuronidation. PCB was also found to cause qualitative changes in the metabolism of 4-methylumbelliferone.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(80)90045-3</identifier><identifier>PMID: 6779821</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Aminophenols - metabolism ; Animals ; Glucuronates - metabolism ; Hymecromone - metabolism ; In Vitro Techniques ; Liver - drug effects ; Liver - metabolism ; Male ; Nitrophenols - metabolism ; Phenobarbital - pharmacology ; Polychlorinated Biphenyls - pharmacology ; Rats ; Sulfates - metabolism</subject><ispartof>Biochemical pharmacology, 1980-11, Vol.29 (21), p.2963-2968</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-7dc606aad3f93c8aa12f2cb9d88525274b952986831b91348b42929e4d3e52723</citedby><cites>FETCH-LOGICAL-c272t-7dc606aad3f93c8aa12f2cb9d88525274b952986831b91348b42929e4d3e52723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-2952(80)90045-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6779821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suolinna, Else-Maj</creatorcontrib><creatorcontrib>Mäntylä, Eero</creatorcontrib><title>Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>The conjugation of 4-methylumbelliferone,
p-nitrophenol and
o-aminophenol in isolated rat liver cells was studied. It was found that for maximum sulphate conjugation to take place a very high sulphate concentration (50 mM) was needed, indicating that the rate limiting step may be the formation of ‘active sulphate’. Under these conditions only a slight increase in the glucuronide/sulphate ratio with increasing substrate concentration was seen. Inducers of drug metabolism. PCB and phenobarbital, increased only glucuronidation. PCB was also found to cause qualitative changes in the metabolism of 4-methylumbelliferone.</description><subject>Aminophenols - metabolism</subject><subject>Animals</subject><subject>Glucuronates - metabolism</subject><subject>Hymecromone - metabolism</subject><subject>In Vitro Techniques</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Nitrophenols - metabolism</subject><subject>Phenobarbital - pharmacology</subject><subject>Polychlorinated Biphenyls - pharmacology</subject><subject>Rats</subject><subject>Sulfates - metabolism</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3DAUhUVpSSdp_0EKWpVm4VYv29Km0A55FALNIl0LWbpOFDSWK8mB_vvKM0OWAYHQPefcq_shdE7JV0po940Q0jVMteyLJBeKENE2_A3aUNnzWu7kW7R5sbxHpzk_rU_Z0RN00vW9koxu0HwdFrukOHkH2EwO5yXMj6YAtnF6Wh5M8XHCvp4cQy07HPwzJGwhhIzHFHersaQY9un5EaY4mDT4YkKDY8J3259NSbCPJlPyB_RuNCHDx-N9hv5cXd5vb5rb39e_tj9uG8t6Vpre2Y50xjg-Km6lMZSNzA7KSdmylvViqFsp2UlOB0W5kINgiikQjkOVGT9Dnw995xT_LpCL3vm8_tpMEJes-1awXglRjeJgtCnmnGDUc_I7k_5pSvQKWq_Y9EpRS6L3oDWvsU_H_suwA_cSOpKt-veDDnXJZw9JZ-thsuB8Alu0i_71Af8B0vyNKQ</recordid><startdate>19801101</startdate><enddate>19801101</enddate><creator>Suolinna, Else-Maj</creator><creator>Mäntylä, Eero</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19801101</creationdate><title>Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats</title><author>Suolinna, Else-Maj ; Mäntylä, Eero</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-7dc606aad3f93c8aa12f2cb9d88525274b952986831b91348b42929e4d3e52723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Aminophenols - metabolism</topic><topic>Animals</topic><topic>Glucuronates - metabolism</topic><topic>Hymecromone - metabolism</topic><topic>In Vitro Techniques</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Nitrophenols - metabolism</topic><topic>Phenobarbital - pharmacology</topic><topic>Polychlorinated Biphenyls - pharmacology</topic><topic>Rats</topic><topic>Sulfates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suolinna, Else-Maj</creatorcontrib><creatorcontrib>Mäntylä, Eero</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suolinna, Else-Maj</au><au>Mäntylä, Eero</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1980-11-01</date><risdate>1980</risdate><volume>29</volume><issue>21</issue><spage>2963</spage><epage>2968</epage><pages>2963-2968</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>The conjugation of 4-methylumbelliferone,
p-nitrophenol and
o-aminophenol in isolated rat liver cells was studied. It was found that for maximum sulphate conjugation to take place a very high sulphate concentration (50 mM) was needed, indicating that the rate limiting step may be the formation of ‘active sulphate’. Under these conditions only a slight increase in the glucuronide/sulphate ratio with increasing substrate concentration was seen. Inducers of drug metabolism. PCB and phenobarbital, increased only glucuronidation. PCB was also found to cause qualitative changes in the metabolism of 4-methylumbelliferone.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>6779821</pmid><doi>10.1016/0006-2952(80)90045-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2952 |
| ispartof | Biochemical pharmacology, 1980-11, Vol.29 (21), p.2963-2968 |
| issn | 0006-2952 1873-2968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75427944 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Aminophenols - metabolism Animals Glucuronates - metabolism Hymecromone - metabolism In Vitro Techniques Liver - drug effects Liver - metabolism Male Nitrophenols - metabolism Phenobarbital - pharmacology Polychlorinated Biphenyls - pharmacology Rats Sulfates - metabolism |
| title | Glucuronide and sulphate conjugation in isolated liver cells from control and phenobarbital- or PCB-treated rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T18%3A16%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glucuronide%20and%20sulphate%20conjugation%20in%20isolated%20liver%20cells%20from%20control%20and%20phenobarbital-%20or%20PCB-treated%20rats&rft.jtitle=Biochemical%20pharmacology&rft.au=Suolinna,%20Else-Maj&rft.date=1980-11-01&rft.volume=29&rft.issue=21&rft.spage=2963&rft.epage=2968&rft.pages=2963-2968&rft.issn=0006-2952&rft.eissn=1873-2968&rft_id=info:doi/10.1016/0006-2952(80)90045-3&rft_dat=%3Cproquest_cross%3E75427944%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75427944&rft_id=info:pmid/6779821&rft_els_id=0006295280900453&rfr_iscdi=true |