4′,5′-dimethoxy-6-carboxyfluorescein: A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays
A new nonfluorescent dye, 4′,5′-dimethoxy-6-carboxyfluorescein has been prepared which is a useful energy acceptor for energy transfer measurements from fluorescein in the study of distances within macromolecular assemblies. The R 0 value of 62 Å for the new dye-fluorescein pair is exceptionally hig...
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| Veröffentlicht in: | Analytical biochemistry 1980-10, Vol.108 (1), p.156-161 |
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| container_title | Analytical biochemistry |
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| creator | Khanna, Pyare L. Ullman, Edwin F. |
| description | A new nonfluorescent dye, 4′,5′-dimethoxy-6-carboxyfluorescein has been prepared which is a useful energy acceptor for energy transfer measurements from fluorescein in the study of distances within macromolecular assemblies. The
R
0 value of 62 Å for the new dye-fluorescein pair is exceptionally high. The utility of this dye pair has been demonstrated in a fluorescence excitation transfer immunoassay for morphine. |
| doi_str_mv | 10.1016/0003-2697(80)90706-X |
| format | Article |
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R
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0 value of 62 Å for the new dye-fluorescein pair is exceptionally high. The utility of this dye pair has been demonstrated in a fluorescence excitation transfer immunoassay for morphine.</description><subject>Antibodies</subject><subject>Energy Transfer</subject><subject>Fluoresceins</subject><subject>Fluorescent Antibody Technique</subject><subject>Indicators and Reagents</subject><subject>Morphine - analysis</subject><subject>Proteins - analysis</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Spectrophotometry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-KFDEQxoMo67j6Bgo5iYLRSnc6Pe1BWBb_wYIXhb2FdKWike5Om3Qvzs1n8FF8JJ_EjDMMePFSVVDfV0n9irGHEp5LkPoFANSi0l37ZAtPO2hBi-tbbCOh0wJq6G6zzUlyl93L-SuAlKrRZ-ysBdCqqTbsp_r949ezpgThwkjLl_h9J7RAm_pS-WGNiTJSmF7yCz7FGxq4C3McSBwSx7jOAzl-kk5InCZKn3d8SXbKnhK3iDQvMfE1k18H7kv5jyGM4zpFm7Pd5fvsjrdDpgfHfM4-vXn98fKduPrw9v3lxZXAumkXgbpT0mOPsoe-6rxqfQu2brHBbaU9NoosSdcrp10rreoqXSMCVVTUru7rc_b4MHdO8dtKeTFjKN8ZBjtRXLNpG1VwdXURqoMQU8w5kTdzCqNNOyPB7E9h9pzNnrPZgvl7CnNdbI-O89d-JHcyHdmX_qtDn8qSN4GSyRj2NFxIhItxMfz_gT_LHJ7l</recordid><startdate>198010</startdate><enddate>198010</enddate><creator>Khanna, Pyare L.</creator><creator>Ullman, Edwin F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198010</creationdate><title>4′,5′-dimethoxy-6-carboxyfluorescein: A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays</title><author>Khanna, Pyare L. ; Ullman, Edwin F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-c6941fcbc1b0b29f47f70a37c5c826fc54eae1db4d6d71a49263cc0e2eb29d3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Antibodies</topic><topic>Energy Transfer</topic><topic>Fluoresceins</topic><topic>Fluorescent Antibody Technique</topic><topic>Indicators and Reagents</topic><topic>Morphine - analysis</topic><topic>Proteins - analysis</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khanna, Pyare L.</creatorcontrib><creatorcontrib>Ullman, Edwin F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khanna, Pyare L.</au><au>Ullman, Edwin F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>4′,5′-dimethoxy-6-carboxyfluorescein: A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1980-10</date><risdate>1980</risdate><volume>108</volume><issue>1</issue><spage>156</spage><epage>161</epage><pages>156-161</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A new nonfluorescent dye, 4′,5′-dimethoxy-6-carboxyfluorescein has been prepared which is a useful energy acceptor for energy transfer measurements from fluorescein in the study of distances within macromolecular assemblies. The
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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1980-10, Vol.108 (1), p.156-161 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75411493 |
| source | ScienceDirect; MEDLINE |
| subjects | Antibodies Energy Transfer Fluoresceins Fluorescent Antibody Technique Indicators and Reagents Morphine - analysis Proteins - analysis Spectrometry, Fluorescence - methods Spectrophotometry |
| title | 4′,5′-dimethoxy-6-carboxyfluorescein: A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays |
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