Effects of HDL Subclasses on 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Human Fibroblasts
Monolayer cultures of normal human fibroblasts were used to study the effects of the main subclasses of high-density lipoproteins, HDL₂ and HDL₃, on 3-hydroxy-3-methylglutaryl CoA reductase (EC 1.1.1.34) activity. In this system, HDL₃ (d=1.1251.210 g/cm³) specifically induced HMG-CoA reductase activ...
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| Veröffentlicht in: | Nutrition and metabolism (Basel) 1980-01, Vol.24, p.26-33 |
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| container_title | Nutrition and metabolism (Basel) |
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| creator | Daerr, W.H. Gianturco, Sandra H. Patsch, J.R. Smith, L.C. Gotto, A.M. |
| description | Monolayer cultures of normal human fibroblasts were used to study the effects of the main subclasses of high-density lipoproteins, HDL₂ and HDL₃, on 3-hydroxy-3-methylglutaryl CoA reductase (EC 1.1.1.34) activity. In this system, HDL₃ (d=1.1251.210 g/cm³) specifically induced HMG-CoA reductase activity. Evaluation of culture dynamics revealed that enzyme induction was restricted to the stationary phase of growth. When the cells were incubated with HDL₂ (d = 1.063-1.125 g/cm³), suppression of reductase activity was observed. Mixtures of HDL₂ and HDL₃ suppressed reductase activity when HDL₂ was greater than 35% of the total HDL. The suppressive effects of HDL₂, were abolished by treatment with cyclohexanedione and restored by regeneration of the arginyl residues, suggesting an apoprotein-mediated suppressive mechanism. These observations show that the cellular effects of HDL depend upon the stage of cell growth and the ratio of HDL subclasses in HDL as usually isolated. |
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In this system, HDL₃ (d=1.1251.210 g/cm³) specifically induced HMG-CoA reductase activity. Evaluation of culture dynamics revealed that enzyme induction was restricted to the stationary phase of growth. When the cells were incubated with HDL₂ (d = 1.063-1.125 g/cm³), suppression of reductase activity was observed. Mixtures of HDL₂ and HDL₃ suppressed reductase activity when HDL₂ was greater than 35% of the total HDL. The suppressive effects of HDL₂, were abolished by treatment with cyclohexanedione and restored by regeneration of the arginyl residues, suggesting an apoprotein-mediated suppressive mechanism. These observations show that the cellular effects of HDL depend upon the stage of cell growth and the ratio of HDL subclasses in HDL as usually isolated.</description><identifier>ISSN: 0029-6678</identifier><identifier>EISSN: 2571-6506</identifier><identifier>PMID: 7454134</identifier><language>eng</language><publisher>Switzerland: S. Karger</publisher><subject>Cell Cycle ; Cells, Cultured ; Cholesterol - biosynthesis ; Enzyme Induction - drug effects ; Fibroblasts - drug effects ; Fibroblasts - enzymology ; Humans ; Hydroxymethylglutaryl CoA Reductases - biosynthesis ; Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent ; Lipoproteins, HDL - physiology ; Lipoproteins, HDL2 ; Lipoproteins, HDL3</subject><ispartof>Nutrition and metabolism (Basel), 1980-01, Vol.24, p.26-33</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/45098988$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/45098988$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,4024,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7454134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Daerr, W.H.</creatorcontrib><creatorcontrib>Gianturco, Sandra H.</creatorcontrib><creatorcontrib>Patsch, J.R.</creatorcontrib><creatorcontrib>Smith, L.C.</creatorcontrib><creatorcontrib>Gotto, A.M.</creatorcontrib><title>Effects of HDL Subclasses on 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Human Fibroblasts</title><title>Nutrition and metabolism (Basel)</title><addtitle>Nutr Metab</addtitle><description>Monolayer cultures of normal human fibroblasts were used to study the effects of the main subclasses of high-density lipoproteins, HDL₂ and HDL₃, on 3-hydroxy-3-methylglutaryl CoA reductase (EC 1.1.1.34) activity. In this system, HDL₃ (d=1.1251.210 g/cm³) specifically induced HMG-CoA reductase activity. Evaluation of culture dynamics revealed that enzyme induction was restricted to the stationary phase of growth. When the cells were incubated with HDL₂ (d = 1.063-1.125 g/cm³), suppression of reductase activity was observed. Mixtures of HDL₂ and HDL₃ suppressed reductase activity when HDL₂ was greater than 35% of the total HDL. The suppressive effects of HDL₂, were abolished by treatment with cyclohexanedione and restored by regeneration of the arginyl residues, suggesting an apoprotein-mediated suppressive mechanism. These observations show that the cellular effects of HDL depend upon the stage of cell growth and the ratio of HDL subclasses in HDL as usually isolated.</description><subject>Cell Cycle</subject><subject>Cells, Cultured</subject><subject>Cholesterol - biosynthesis</subject><subject>Enzyme Induction - drug effects</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - enzymology</subject><subject>Humans</subject><subject>Hydroxymethylglutaryl CoA Reductases - biosynthesis</subject><subject>Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent</subject><subject>Lipoproteins, HDL - physiology</subject><subject>Lipoproteins, HDL2</subject><subject>Lipoproteins, HDL3</subject><issn>0029-6678</issn><issn>2571-6506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLxDAUhYMo46DzE4Ss3AXSJM1jOYwzVhgRfKxLmtxohz7GJgXrr7fgoHdz4H6HA-ecoSXLVUZkTuU5WlLKDJFS6Uu0ivFA55sJY3KBFkrkIuNiiew2BHAp4j7g4m6PX8bKNTZGmD8d5qSY_NB_TYSTR0gfU_PejMkOU4M3PXTfUwt4jZ_Bjy7ZCLjucDG2tsO7uhr6ag5K8RpdBNtEWJ30Cr3ttq-bguyf7h826z05MKYT4UKL3GVMZFkwQB1kQUjPhecBtMu8z60C5YyRnDJaSSWsYBCs4UCttZ5fodvf3OPQf44QU9nW0UHT2A76MZYqF1QzZWbjzck4Vi348jjU7VypPG3yzw8x9cMfFjk12mjNfwCrG2iV</recordid><startdate>19800101</startdate><enddate>19800101</enddate><creator>Daerr, W.H.</creator><creator>Gianturco, Sandra H.</creator><creator>Patsch, J.R.</creator><creator>Smith, L.C.</creator><creator>Gotto, A.M.</creator><general>S. 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In this system, HDL₃ (d=1.1251.210 g/cm³) specifically induced HMG-CoA reductase activity. Evaluation of culture dynamics revealed that enzyme induction was restricted to the stationary phase of growth. When the cells were incubated with HDL₂ (d = 1.063-1.125 g/cm³), suppression of reductase activity was observed. Mixtures of HDL₂ and HDL₃ suppressed reductase activity when HDL₂ was greater than 35% of the total HDL. The suppressive effects of HDL₂, were abolished by treatment with cyclohexanedione and restored by regeneration of the arginyl residues, suggesting an apoprotein-mediated suppressive mechanism. These observations show that the cellular effects of HDL depend upon the stage of cell growth and the ratio of HDL subclasses in HDL as usually isolated.</abstract><cop>Switzerland</cop><pub>S. Karger</pub><pmid>7454134</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Nutrition and metabolism (Basel), 1980-01, Vol.24, p.26-33 |
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| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Cell Cycle Cells, Cultured Cholesterol - biosynthesis Enzyme Induction - drug effects Fibroblasts - drug effects Fibroblasts - enzymology Humans Hydroxymethylglutaryl CoA Reductases - biosynthesis Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent Lipoproteins, HDL - physiology Lipoproteins, HDL2 Lipoproteins, HDL3 |
| title | Effects of HDL Subclasses on 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Human Fibroblasts |
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