Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity
The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2....
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| Veröffentlicht in: | Biochemical pharmacology 1980-12, Vol.29 (23), p.3175-3180 |
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| container_issue | 23 |
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| container_title | Biochemical pharmacology |
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| creator | Mezey, Esteban Potter, James J. Harmon, S.Mitchell Tsitouras, Panayiotis D. |
| description | The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.)
mmoles
hr
as compared with a value of 1.39 ± 0.125
mmoles
hr
in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD
+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination. |
| doi_str_mv | 10.1016/0006-2952(80)90582-1 |
| format | Article |
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mmoles
hr
as compared with a value of 1.39 ± 0.125
mmoles
hr
in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD
+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(80)90582-1</identifier><identifier>PMID: 7004445</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Alcohol Oxidoreductases - metabolism ; Animals ; Castration ; Cytosol - analysis ; Ethanol - metabolism ; Liver - drug effects ; Liver - enzymology ; Male ; Mitochondria, Liver - analysis ; NAD - metabolism ; Rats ; Testosterone - blood ; Testosterone - pharmacology</subject><ispartof>Biochemical pharmacology, 1980-12, Vol.29 (23), p.3175-3180</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-bdb4a5870ea7f353c33c238966805ae734ba731a3b6c3b32945a8ec52a85546b3</citedby><cites>FETCH-LOGICAL-c357t-bdb4a5870ea7f353c33c238966805ae734ba731a3b6c3b32945a8ec52a85546b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006295280905821$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7004445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mezey, Esteban</creatorcontrib><creatorcontrib>Potter, James J.</creatorcontrib><creatorcontrib>Harmon, S.Mitchell</creatorcontrib><creatorcontrib>Tsitouras, Panayiotis D.</creatorcontrib><title>Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.)
mmoles
hr
as compared with a value of 1.39 ± 0.125
mmoles
hr
in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD
+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.</description><subject>Alcohol Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>Castration</subject><subject>Cytosol - analysis</subject><subject>Ethanol - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Mitochondria, Liver - analysis</subject><subject>NAD - metabolism</subject><subject>Rats</subject><subject>Testosterone - blood</subject><subject>Testosterone - pharmacology</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYMotVa_gcKeRA-rySbZzV4EKf6Dghc9h9nsrI1sNzVJC_32prb0KASSzHtvMvkRcsnoHaOsvKeUlnlRy-JG0duaSlXk7IiMmap4KpfqmIwPllNyFsL39qpKNiKjilIhhBwTeOo6NDFkrssMhOghWjdkMLRZxBBdiOjdgBm0CzvYg55WOmW9XaPPoDdu7vqsxfmm9e4LBwgpYaJd27g5Jycd9AEv9vuEfD4_fUxf89n7y9v0cZYbLquYN20jQKqKIlQdl9xwbgqu6rJUVAJWXDRQcQa8KQ1veFELCQqNLEBJKcqGT8j1ru_Su59Vml0vbDDY9zCgWwVdSUFFQetkFDuj8S4Ej51eersAv9GM6i1ZveWkt9i0ovqPrGYpdrXvv2oW2B5Ce5RJf9jpmD65tuh1MBYHg631ibBunf3_gV_HRIkO</recordid><startdate>19801201</startdate><enddate>19801201</enddate><creator>Mezey, Esteban</creator><creator>Potter, James J.</creator><creator>Harmon, S.Mitchell</creator><creator>Tsitouras, Panayiotis D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19801201</creationdate><title>Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity</title><author>Mezey, Esteban ; Potter, James J. ; Harmon, S.Mitchell ; Tsitouras, Panayiotis D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-bdb4a5870ea7f353c33c238966805ae734ba731a3b6c3b32945a8ec52a85546b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Alcohol Oxidoreductases - metabolism</topic><topic>Animals</topic><topic>Castration</topic><topic>Cytosol - analysis</topic><topic>Ethanol - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Mitochondria, Liver - analysis</topic><topic>NAD - metabolism</topic><topic>Rats</topic><topic>Testosterone - blood</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mezey, Esteban</creatorcontrib><creatorcontrib>Potter, James J.</creatorcontrib><creatorcontrib>Harmon, S.Mitchell</creatorcontrib><creatorcontrib>Tsitouras, Panayiotis D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mezey, Esteban</au><au>Potter, James J.</au><au>Harmon, S.Mitchell</au><au>Tsitouras, Panayiotis D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1980-12-01</date><risdate>1980</risdate><volume>29</volume><issue>23</issue><spage>3175</spage><epage>3180</epage><pages>3175-3180</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.)
mmoles
hr
as compared with a value of 1.39 ± 0.125
mmoles
hr
in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD
+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>7004445</pmid><doi>10.1016/0006-2952(80)90582-1</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 1980-12, Vol.29 (23), p.3175-3180 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alcohol Oxidoreductases - metabolism Animals Castration Cytosol - analysis Ethanol - metabolism Liver - drug effects Liver - enzymology Male Mitochondria, Liver - analysis NAD - metabolism Rats Testosterone - blood Testosterone - pharmacology |
| title | Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity |
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