Proteomic and activity profiles of ascorbate–glutathione cycle enzymes in germinating barley embryo

Enzymes involved in redox control are important during seed germination and seedling growth. Ascorbate–glutathione cycle enzymes in barley embryo extracts were monitored both by 2D-gel electrophoresis and activity measurements from 4 to 144 h post imbibition (PI). Strikingly different activity profi...

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Veröffentlicht in:	Phytochemistry (Oxford) 2010-10, Vol.71 (14), p.1650-1656
Hauptverfasser:	Bønsager, Birgit C, Shahpiri, Azar, Finnie, Christine, Svensson, Birte
Format:	Artikel
Sprache:	eng
Schlagworte:	ascorbate peroxidase Ascorbate Peroxidases Ascorbic Acid - analysis Ascorbic Acid - metabolism barley Biological and medical sciences Cereal grain proteome Chemical constitution Electrophoresis, Gel, Two-Dimensional Enzyme assays Fundamental and applied biological sciences. Psychology Germination - physiology Glutathione - analysis Glutathione - metabolism glutathione dehydrogenase (ascorbate) Glutathione Reductase - metabolism glutathione-disulfide reductase Hordeum - embryology Hordeum - enzymology Hordeum - metabolism Hordeum vulgare subsp. vulgare mass spectrometry monodehydroascorbate reductase (NADH) Oxidoreductases - metabolism Peroxidases - metabolism Plant physiology and development proteomics Redox regulation Seed germination seedling growth Seeds - enzymology Seeds - metabolism Two-dimensional gel electrophoresis
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