Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry
An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2010, Vol.72(8), pp.1063-1066 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1066 |
|---|---|
| container_issue | 8 |
| container_start_page | 1063 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 72 |
| creator | TSUCHIYA, Ryo KOMATSU, Takahiro ISHIKAWA, Takefumi NEO, Sakurako MCCONNELL, Mary F. HISASUE, Masaharu YAMADA, Takatsugu |
| description | An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to detect the PSA proteins. IgM single, IgM/C3 double and IgG/IgM/C3 triple positive platelets were prepared. FITC-conjugated anti-IgG antibody bound strongly only to the triple positive platelets. Binding of FITC-conjugated anti-IgM or anti-C3 antibody to the double and triple positive platelets was specifically blocked by preincubation with the respective non-FITC-conjugated same-origin antibodies. These results confirm that FITC-conjugated antibodies specifically detect PSA proteins and that the control platelets prepared in this study are appropriate positive controls for detection of PSA proteins by flow cytometry. |
| doi_str_mv | 10.1292/jvms.09-0405 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754024236</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3158671181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-44cf8b0ea7ec937ebeeab7903a4e8340b73982d96034b0679f6592591072436c3</originalsourceid><addsrcrecordid>eNpd0U9v0zAYBnALgVg3uHFGljgA0jJe_0kcH0fGxqQhKgFny3HfjFRJ3NnOUL8DHxpX7Xrg8lqyf3ps-SHkDYMLxjX_tH4c4wXoAiSUz8iCCakKJYV-ThagWVUoXsIJOY1xDcCZrPRLcsKBC8lYuSB_lwE3NtjU-4n6ji597FP_iLTxUwp-oMvBJhwwRdr5QK8woXuyjZ36CY-C_phDZx0WlzF61-fNFb29vznP4xu10ypHjpsBR5wS_dCIj7Td0uvB_6HNNvkRU9i-Ii86O0R8fVjPyK_rLz-br8Xd95vb5vKucKWEVEjpuroFtAqdFgpbRNsqDcJKrIWEVgld85WuQMgWKqW7qtS81AwUl6Jy4oy83-dugn-YMSYz9tHhMNgJ_RyNytdwyUWV5bv_5NrPYcqPM_kra8U0aJ3V-V654GMM2JlN6EcbtoaB2ZVkdiUZ0GZXUuZvD6FzO-LqiJ9ayeDzHqxjsvd4BDak3g24T1Pc1LtxSD0eut82GJzEP9xspHY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468719099</pqid></control><display><type>article</type><title>Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>TSUCHIYA, Ryo ; KOMATSU, Takahiro ; ISHIKAWA, Takefumi ; NEO, Sakurako ; MCCONNELL, Mary F. ; HISASUE, Masaharu ; YAMADA, Takatsugu</creator><creatorcontrib>TSUCHIYA, Ryo ; KOMATSU, Takahiro ; ISHIKAWA, Takefumi ; NEO, Sakurako ; MCCONNELL, Mary F. ; HISASUE, Masaharu ; YAMADA, Takatsugu</creatorcontrib><description>An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to detect the PSA proteins. IgM single, IgM/C3 double and IgG/IgM/C3 triple positive platelets were prepared. FITC-conjugated anti-IgG antibody bound strongly only to the triple positive platelets. Binding of FITC-conjugated anti-IgM or anti-C3 antibody to the double and triple positive platelets was specifically blocked by preincubation with the respective non-FITC-conjugated same-origin antibodies. These results confirm that FITC-conjugated antibodies specifically detect PSA proteins and that the control platelets prepared in this study are appropriate positive controls for detection of PSA proteins by flow cytometry.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.09-0405</identifier><identifier>PMID: 20234115</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Blood Platelets - drug effects ; Blood Platelets - immunology ; canine ; Cell Membrane - immunology ; Complement C3 - metabolism ; Dogs ; Female ; Flow Cytometry ; Fluorescein-5-isothiocyanate ; immune-mediated thrombocytopenia ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Lipopolysaccharides - pharmacology ; Male ; Platelet Count - veterinary ; platelet surface-associated immunological proteins ; positive control</subject><ispartof>Journal of Veterinary Medical Science, 2010, Vol.72(8), pp.1063-1066</ispartof><rights>2010 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-44cf8b0ea7ec937ebeeab7903a4e8340b73982d96034b0679f6592591072436c3</citedby><cites>FETCH-LOGICAL-c540t-44cf8b0ea7ec937ebeeab7903a4e8340b73982d96034b0679f6592591072436c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20234115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TSUCHIYA, Ryo</creatorcontrib><creatorcontrib>KOMATSU, Takahiro</creatorcontrib><creatorcontrib>ISHIKAWA, Takefumi</creatorcontrib><creatorcontrib>NEO, Sakurako</creatorcontrib><creatorcontrib>MCCONNELL, Mary F.</creatorcontrib><creatorcontrib>HISASUE, Masaharu</creatorcontrib><creatorcontrib>YAMADA, Takatsugu</creatorcontrib><title>Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to detect the PSA proteins. IgM single, IgM/C3 double and IgG/IgM/C3 triple positive platelets were prepared. FITC-conjugated anti-IgG antibody bound strongly only to the triple positive platelets. Binding of FITC-conjugated anti-IgM or anti-C3 antibody to the double and triple positive platelets was specifically blocked by preincubation with the respective non-FITC-conjugated same-origin antibodies. These results confirm that FITC-conjugated antibodies specifically detect PSA proteins and that the control platelets prepared in this study are appropriate positive controls for detection of PSA proteins by flow cytometry.</description><subject>Animals</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - immunology</subject><subject>canine</subject><subject>Cell Membrane - immunology</subject><subject>Complement C3 - metabolism</subject><subject>Dogs</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>immune-mediated thrombocytopenia</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Platelet Count - veterinary</subject><subject>platelet surface-associated immunological proteins</subject><subject>positive control</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0U9v0zAYBnALgVg3uHFGljgA0jJe_0kcH0fGxqQhKgFny3HfjFRJ3NnOUL8DHxpX7Xrg8lqyf3ps-SHkDYMLxjX_tH4c4wXoAiSUz8iCCakKJYV-ThagWVUoXsIJOY1xDcCZrPRLcsKBC8lYuSB_lwE3NtjU-4n6ji597FP_iLTxUwp-oMvBJhwwRdr5QK8woXuyjZ36CY-C_phDZx0WlzF61-fNFb29vznP4xu10ypHjpsBR5wS_dCIj7Td0uvB_6HNNvkRU9i-Ii86O0R8fVjPyK_rLz-br8Xd95vb5vKucKWEVEjpuroFtAqdFgpbRNsqDcJKrIWEVgld85WuQMgWKqW7qtS81AwUl6Jy4oy83-dugn-YMSYz9tHhMNgJ_RyNytdwyUWV5bv_5NrPYcqPM_kra8U0aJ3V-V654GMM2JlN6EcbtoaB2ZVkdiUZ0GZXUuZvD6FzO-LqiJ9ayeDzHqxjsvd4BDak3g24T1Pc1LtxSD0eut82GJzEP9xspHY</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>TSUCHIYA, Ryo</creator><creator>KOMATSU, Takahiro</creator><creator>ISHIKAWA, Takefumi</creator><creator>NEO, Sakurako</creator><creator>MCCONNELL, Mary F.</creator><creator>HISASUE, Masaharu</creator><creator>YAMADA, Takatsugu</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20100801</creationdate><title>Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry</title><author>TSUCHIYA, Ryo ; KOMATSU, Takahiro ; ISHIKAWA, Takefumi ; NEO, Sakurako ; MCCONNELL, Mary F. ; HISASUE, Masaharu ; YAMADA, Takatsugu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-44cf8b0ea7ec937ebeeab7903a4e8340b73982d96034b0679f6592591072436c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - immunology</topic><topic>canine</topic><topic>Cell Membrane - immunology</topic><topic>Complement C3 - metabolism</topic><topic>Dogs</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>immune-mediated thrombocytopenia</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Platelet Count - veterinary</topic><topic>platelet surface-associated immunological proteins</topic><topic>positive control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TSUCHIYA, Ryo</creatorcontrib><creatorcontrib>KOMATSU, Takahiro</creatorcontrib><creatorcontrib>ISHIKAWA, Takefumi</creatorcontrib><creatorcontrib>NEO, Sakurako</creatorcontrib><creatorcontrib>MCCONNELL, Mary F.</creatorcontrib><creatorcontrib>HISASUE, Masaharu</creatorcontrib><creatorcontrib>YAMADA, Takatsugu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TSUCHIYA, Ryo</au><au>KOMATSU, Takahiro</au><au>ISHIKAWA, Takefumi</au><au>NEO, Sakurako</au><au>MCCONNELL, Mary F.</au><au>HISASUE, Masaharu</au><au>YAMADA, Takatsugu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>72</volume><issue>8</issue><spage>1063</spage><epage>1066</epage><pages>1063-1066</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to detect the PSA proteins. IgM single, IgM/C3 double and IgG/IgM/C3 triple positive platelets were prepared. FITC-conjugated anti-IgG antibody bound strongly only to the triple positive platelets. Binding of FITC-conjugated anti-IgM or anti-C3 antibody to the double and triple positive platelets was specifically blocked by preincubation with the respective non-FITC-conjugated same-origin antibodies. These results confirm that FITC-conjugated antibodies specifically detect PSA proteins and that the control platelets prepared in this study are appropriate positive controls for detection of PSA proteins by flow cytometry.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>20234115</pmid><doi>10.1292/jvms.09-0405</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2010, Vol.72(8), pp.1063-1066 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_754024236 |
| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Blood Platelets - drug effects Blood Platelets - immunology canine Cell Membrane - immunology Complement C3 - metabolism Dogs Female Flow Cytometry Fluorescein-5-isothiocyanate immune-mediated thrombocytopenia Immunoglobulin G - blood Immunoglobulin M - blood Lipopolysaccharides - pharmacology Male Platelet Count - veterinary platelet surface-associated immunological proteins positive control |
| title | Preparation of Positive Control Platelets for Detection of Canine Platelet Surface-Associated IgG, IgM and Complement (C3) by Flow Cytometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T09%3A21%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20of%20Positive%20Control%20Platelets%20for%20Detection%20of%20Canine%20Platelet%20Surface-Associated%20IgG,%20IgM%20and%20Complement%20(C3)%20by%20Flow%20Cytometry&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=TSUCHIYA,%20Ryo&rft.date=2010-08-01&rft.volume=72&rft.issue=8&rft.spage=1063&rft.epage=1066&rft.pages=1063-1066&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.09-0405&rft_dat=%3Cproquest_cross%3E3158671181%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468719099&rft_id=info:pmid/20234115&rfr_iscdi=true |