The antiepileptogenic effect of electrical stimulation at different low frequencies is accompanied with change in adenosine receptors gene expression in rats
Summary Purpose: Previous studies have shown that the anticonvulsant effects of low‐frequency stimulation (LFS) can be affected by activation of adenosine receptors. In the present study, the effect of LFS at different frequencies on kindling rate and adenosine receptors gene expression was investi...
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| Veröffentlicht in: | Epilepsia (Copenhagen) 2009-07, Vol.50 (7), p.1768-1779 |
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| creator | Jahanshahi, A. Mirnajafi‐Zadeh, Javad Javan, Mohammad Mohammad‐Zadeh, Mohammad Rohani, Razieh |
| description | Summary
Purpose: Previous studies have shown that the anticonvulsant effects of low‐frequency stimulation (LFS) can be affected by activation of adenosine receptors. In the present study, the effect of LFS at different frequencies on kindling rate and adenosine receptors gene expression was investigated.
Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner. LFS (0.5, 1, and 5 Hz) was applied after termination of each kindling stimulation. Seizure severity was measured according to behavioral and electrophysiologic parameters. At the end of the experiments, adenosine A1 and A2A receptor gene expression were measured.
Results: The inhibitory effect of LFS on kindling acquisition was observed at all frequencies. In addition, the inhibitory action of LFS on enhancement of field excitatory postsynaptic potential slope and population spike amplitude during kindling acquisition was not affected by the LFS frequency. However, the effects of LFS on paired‐pulse recordings were greater at frequency of 5 Hz. Application of LFS during kindling acquisition also prevented the kindling induced decrease in the A1 receptor gene expression and attenuated the level of A2A receptor gene expression in the dentate gyrus. These effects were also greater at the frequency of 5 Hz.
Discussion: According to these data, it may be suggested that the antiepileptogenic effects of LFS, developed through inhibition of synaptic transmission in the dentate gyrus, is mediated somehow through preventing the decrease of A1 receptor and through attenuating the A2A receptor gene expression. These effects might be dependent on the frequency of LFS. |
| doi_str_mv | 10.1111/j.1528-1167.2009.02088.x |
| format | Article |
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Purpose: Previous studies have shown that the anticonvulsant effects of low‐frequency stimulation (LFS) can be affected by activation of adenosine receptors. In the present study, the effect of LFS at different frequencies on kindling rate and adenosine receptors gene expression was investigated.
Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner. LFS (0.5, 1, and 5 Hz) was applied after termination of each kindling stimulation. Seizure severity was measured according to behavioral and electrophysiologic parameters. At the end of the experiments, adenosine A1 and A2A receptor gene expression were measured.
Results: The inhibitory effect of LFS on kindling acquisition was observed at all frequencies. In addition, the inhibitory action of LFS on enhancement of field excitatory postsynaptic potential slope and population spike amplitude during kindling acquisition was not affected by the LFS frequency. However, the effects of LFS on paired‐pulse recordings were greater at frequency of 5 Hz. Application of LFS during kindling acquisition also prevented the kindling induced decrease in the A1 receptor gene expression and attenuated the level of A2A receptor gene expression in the dentate gyrus. These effects were also greater at the frequency of 5 Hz.
Discussion: According to these data, it may be suggested that the antiepileptogenic effects of LFS, developed through inhibition of synaptic transmission in the dentate gyrus, is mediated somehow through preventing the decrease of A1 receptor and through attenuating the A2A receptor gene expression. These effects might be dependent on the frequency of LFS.</description><identifier>ISSN: 0013-9580</identifier><identifier>EISSN: 1528-1167</identifier><identifier>DOI: 10.1111/j.1528-1167.2009.02088.x</identifier><identifier>PMID: 19453712</identifier><identifier>CODEN: EPILAK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenosine receptors ; Animals ; Anticonvulsants. Antiepileptics. Antiparkinson agents ; Biological and medical sciences ; Dentate gyrus ; Dentate Gyrus - physiology ; Disease Models, Animal ; Electric Stimulation - methods ; Excitatory Postsynaptic Potentials - physiology ; Gene Expression ; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy ; Kindling, Neurologic - physiology ; Low‐frequency stimulation ; Male ; Medical sciences ; Nervous system (semeiology, syndromes) ; Neurology ; Neuropharmacology ; Perforant Pathway - physiology ; Pharmacology. Drug treatments ; Rats ; Rats, Wistar ; Receptors, Purinergic P1 - genetics ; Receptors, Purinergic P1 - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Seizure ; Seizures - etiology ; Seizures - physiopathology ; Seizures - prevention & control ; Synaptic Transmission - physiology</subject><ispartof>Epilepsia (Copenhagen), 2009-07, Vol.50 (7), p.1768-1779</ispartof><rights>Wiley Periodicals, Inc. © 2009 International League Against Epilepsy</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4798-74faab21a297fb4a836862fcaed6950c268616bd7a8b9959b898760e2c9eec33</citedby><cites>FETCH-LOGICAL-c4798-74faab21a297fb4a836862fcaed6950c268616bd7a8b9959b898760e2c9eec33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1528-1167.2009.02088.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1528-1167.2009.02088.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21693434$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19453712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jahanshahi, A.</creatorcontrib><creatorcontrib>Mirnajafi‐Zadeh, Javad</creatorcontrib><creatorcontrib>Javan, Mohammad</creatorcontrib><creatorcontrib>Mohammad‐Zadeh, Mohammad</creatorcontrib><creatorcontrib>Rohani, Razieh</creatorcontrib><title>The antiepileptogenic effect of electrical stimulation at different low frequencies is accompanied with change in adenosine receptors gene expression in rats</title><title>Epilepsia (Copenhagen)</title><addtitle>Epilepsia</addtitle><description>Summary
Purpose: Previous studies have shown that the anticonvulsant effects of low‐frequency stimulation (LFS) can be affected by activation of adenosine receptors. In the present study, the effect of LFS at different frequencies on kindling rate and adenosine receptors gene expression was investigated.
Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner. LFS (0.5, 1, and 5 Hz) was applied after termination of each kindling stimulation. Seizure severity was measured according to behavioral and electrophysiologic parameters. At the end of the experiments, adenosine A1 and A2A receptor gene expression were measured.
Results: The inhibitory effect of LFS on kindling acquisition was observed at all frequencies. In addition, the inhibitory action of LFS on enhancement of field excitatory postsynaptic potential slope and population spike amplitude during kindling acquisition was not affected by the LFS frequency. However, the effects of LFS on paired‐pulse recordings were greater at frequency of 5 Hz. Application of LFS during kindling acquisition also prevented the kindling induced decrease in the A1 receptor gene expression and attenuated the level of A2A receptor gene expression in the dentate gyrus. These effects were also greater at the frequency of 5 Hz.
Discussion: According to these data, it may be suggested that the antiepileptogenic effects of LFS, developed through inhibition of synaptic transmission in the dentate gyrus, is mediated somehow through preventing the decrease of A1 receptor and through attenuating the A2A receptor gene expression. These effects might be dependent on the frequency of LFS.</description><subject>Adenosine receptors</subject><subject>Animals</subject><subject>Anticonvulsants. Antiepileptics. Antiparkinson agents</subject><subject>Biological and medical sciences</subject><subject>Dentate gyrus</subject><subject>Dentate Gyrus - physiology</subject><subject>Disease Models, Animal</subject><subject>Electric Stimulation - methods</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>Gene Expression</subject><subject>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</subject><subject>Kindling, Neurologic - physiology</subject><subject>Low‐frequency stimulation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Neuropharmacology</subject><subject>Perforant Pathway - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Purinergic P1 - genetics</subject><subject>Receptors, Purinergic P1 - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Seizure</subject><subject>Seizures - etiology</subject><subject>Seizures - physiopathology</subject><subject>Seizures - prevention & control</subject><subject>Synaptic Transmission - physiology</subject><issn>0013-9580</issn><issn>1528-1167</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P1CAYgInRuOPqXzBc1FMrH22BgwezWXWTTfQwd0Lpyw6TllZgMrM_xv8qdSbrzciFl_C8H_AghCmpaVkf9zVtmawo7UTNCFE1YUTK-vQMbZ4unqMNIZRXqpXkCr1KaU8IEZ3gL9EVVU3LBWUb9Gu7A2xC9rD4EZY8P0DwFoNzYDOeHYaxBNFbM-KU_XQYTfZzwCbjwRcoQsh4nI_YRfh5gGA9JOwTNtbO02KChwEffd5huzPhAbAvqQOEOfkAOIJdW8aES1fAcFoipLSWL1g0Ob1GL5wZE7y57Ndo--V2e_Otuv_-9e7m831lG6FkJRpnTM-oYUq4vjGSd7JjzhoYOtUSy8qRdv0gjOyValUvlRQdAWYVgOX8Gn04l13iXB6Rsp58sjCOJsB8SFq0DSEdIbKQ7_9JMkIVF6IpoDyDNs4pRXB6iX4y8VFToleHeq9XVXpVpVeH-o9DfSqpby89Dv0Ew9_Ei7QCvLsAJhUvLpry7emJY7RTvOHrDJ_O3LGoffzvAfTtj7s14r8BrM-7UQ</recordid><startdate>200907</startdate><enddate>200907</enddate><creator>Jahanshahi, A.</creator><creator>Mirnajafi‐Zadeh, Javad</creator><creator>Javan, Mohammad</creator><creator>Mohammad‐Zadeh, Mohammad</creator><creator>Rohani, Razieh</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200907</creationdate><title>The antiepileptogenic effect of electrical stimulation at different low frequencies is accompanied with change in adenosine receptors gene expression in rats</title><author>Jahanshahi, A. ; Mirnajafi‐Zadeh, Javad ; Javan, Mohammad ; Mohammad‐Zadeh, Mohammad ; Rohani, Razieh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4798-74faab21a297fb4a836862fcaed6950c268616bd7a8b9959b898760e2c9eec33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenosine receptors</topic><topic>Animals</topic><topic>Anticonvulsants. Antiepileptics. Antiparkinson agents</topic><topic>Biological and medical sciences</topic><topic>Dentate gyrus</topic><topic>Dentate Gyrus - physiology</topic><topic>Disease Models, Animal</topic><topic>Electric Stimulation - methods</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>Gene Expression</topic><topic>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</topic><topic>Kindling, Neurologic - physiology</topic><topic>Low‐frequency stimulation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Neuropharmacology</topic><topic>Perforant Pathway - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Purinergic P1 - genetics</topic><topic>Receptors, Purinergic P1 - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Seizure</topic><topic>Seizures - etiology</topic><topic>Seizures - physiopathology</topic><topic>Seizures - prevention & control</topic><topic>Synaptic Transmission - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jahanshahi, A.</creatorcontrib><creatorcontrib>Mirnajafi‐Zadeh, Javad</creatorcontrib><creatorcontrib>Javan, Mohammad</creatorcontrib><creatorcontrib>Mohammad‐Zadeh, Mohammad</creatorcontrib><creatorcontrib>Rohani, Razieh</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Epilepsia (Copenhagen)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jahanshahi, A.</au><au>Mirnajafi‐Zadeh, Javad</au><au>Javan, Mohammad</au><au>Mohammad‐Zadeh, Mohammad</au><au>Rohani, Razieh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The antiepileptogenic effect of electrical stimulation at different low frequencies is accompanied with change in adenosine receptors gene expression in rats</atitle><jtitle>Epilepsia (Copenhagen)</jtitle><addtitle>Epilepsia</addtitle><date>2009-07</date><risdate>2009</risdate><volume>50</volume><issue>7</issue><spage>1768</spage><epage>1779</epage><pages>1768-1779</pages><issn>0013-9580</issn><eissn>1528-1167</eissn><coden>EPILAK</coden><abstract>Summary
Purpose: Previous studies have shown that the anticonvulsant effects of low‐frequency stimulation (LFS) can be affected by activation of adenosine receptors. In the present study, the effect of LFS at different frequencies on kindling rate and adenosine receptors gene expression was investigated.
Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner. LFS (0.5, 1, and 5 Hz) was applied after termination of each kindling stimulation. Seizure severity was measured according to behavioral and electrophysiologic parameters. At the end of the experiments, adenosine A1 and A2A receptor gene expression were measured.
Results: The inhibitory effect of LFS on kindling acquisition was observed at all frequencies. In addition, the inhibitory action of LFS on enhancement of field excitatory postsynaptic potential slope and population spike amplitude during kindling acquisition was not affected by the LFS frequency. However, the effects of LFS on paired‐pulse recordings were greater at frequency of 5 Hz. Application of LFS during kindling acquisition also prevented the kindling induced decrease in the A1 receptor gene expression and attenuated the level of A2A receptor gene expression in the dentate gyrus. These effects were also greater at the frequency of 5 Hz.
Discussion: According to these data, it may be suggested that the antiepileptogenic effects of LFS, developed through inhibition of synaptic transmission in the dentate gyrus, is mediated somehow through preventing the decrease of A1 receptor and through attenuating the A2A receptor gene expression. These effects might be dependent on the frequency of LFS.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19453712</pmid><doi>10.1111/j.1528-1167.2009.02088.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine receptors Animals Anticonvulsants. Antiepileptics. Antiparkinson agents Biological and medical sciences Dentate gyrus Dentate Gyrus - physiology Disease Models, Animal Electric Stimulation - methods Excitatory Postsynaptic Potentials - physiology Gene Expression Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy Kindling, Neurologic - physiology Low‐frequency stimulation Male Medical sciences Nervous system (semeiology, syndromes) Neurology Neuropharmacology Perforant Pathway - physiology Pharmacology. Drug treatments Rats Rats, Wistar Receptors, Purinergic P1 - genetics Receptors, Purinergic P1 - physiology Reverse Transcriptase Polymerase Chain Reaction Seizure Seizures - etiology Seizures - physiopathology Seizures - prevention & control Synaptic Transmission - physiology |
| title | The antiepileptogenic effect of electrical stimulation at different low frequencies is accompanied with change in adenosine receptors gene expression in rats |
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