Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States
We used PCR and a novel serologic assay to determine infection and exposure rates to Ehrlichia ewingii in dogs from an area of northeast Oklahoma and northwest Arkansas where Amblyomma americanum ticks are abundant. Of 143 dogs assayed, 13 (9.1%) harbored E. ewingii by PCR and 64 (44.8%) had antibod...
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| Veröffentlicht in: | Veterinary parasitology 2010-09, Vol.172 (3), p.355-360 |
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| creator | Little, Susan E. O’Connor, Thomas P. Hempstead, Julie Saucier, Jill Reichard, Mason V. Meinkoth, Katrina Meinkoth, James H. Andrews, Blaine Ullom, Steve Ewing, Sidney A. Chandrashekar, Ramaswamy |
| description | We used PCR and a novel serologic assay to determine infection and exposure rates to
Ehrlichia ewingii in dogs from an area of northeast Oklahoma and northwest Arkansas where
Amblyomma americanum ticks are abundant. Of 143 dogs assayed, 13 (9.1%) harbored
E. ewingii by PCR and 64 (44.8%) had antibodies to
E. ewingii detected using a peptide-based microtiter plate ELISA. Dogs were more likely (
P
=
0.001) to be positive by PCR if sampled in August (30.8%) but no association was found between seropositive status and month of collection of sample (
P
>
0.05). Additional testing revealed PCR evidence of
Ehrlichia chaffeensis (4/143; 2.8%) and
Anaplasma platys (5/143; 3.5%) as well as antibodies reactive to
E. chaffeensis (25/143; 17.5%),
Ehrlichia canis (2/143; 1.4%), and
Anaplasma spp. (8/143; 5.6%). Testing of another 200 dogs from the area revealed additional PCR and/or serologic evidence of
E. ewingii,
E. canis,
E. chaffeensis, and
A. platys. None of the 343 dogs evaluated had evidence of
Borrelia burgdorferi exposure. These data support the interpretation that
E. ewingii may be the primary agent of canine ehrlichiosis in this region, and suggest that diagnostic evaluation of dogs suspected to have a tick-borne disease should include assays targeting this organism. |
| doi_str_mv | 10.1016/j.vetpar.2010.05.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_753996946</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304401710002852</els_id><sourcerecordid>753996946</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-c71721ec74adcef41c234eb30caac3a55e243272a423f5e10b279a6f94ec9d503</originalsourceid><addsrcrecordid>eNp9kE1vEzEQhq0K1KaBf4DAN06bjr_WuxckVJWCVIlDm0svluOdTRxt1sH2lvbf42gLR04jzTwz8-oh5AODFQNWX-1XT5iPNq44lBaoFUB9Rhas0aLiSsEbsgABspLA9AW5TGkPABJqfU4uOCjJBOcL8nizi4N3O28p_vbj1nvqxx5d9mGkduwoPh9DmiLSaDOmMqRd2Cbax3CgeYc0hSnvHI452oGuR5-xo_f5xL4jb3s7JHz_Wpdk_e3m4fp7dffz9sf117vKiUblymmmOUOnpe0c9pI5LiRuBDhrnbBKIZeCa24lF71CBhuuW1v3rUTXdgrEknye7x5j-DVhyubgk8NhsCOGKRmtRNvWrawLKWfSxZBSxN4coz_Y-GIYmJNUszezVHOSakCZIrWsfXx9MG0O2P1b-muxAJ9moLfB2G30yazvywUBrGnqpgRYki8zgUXEk8dokvM4Oux8LLJNF_z_M_wBPdKUbg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>753996946</pqid></control><display><type>article</type><title>Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Little, Susan E. ; O’Connor, Thomas P. ; Hempstead, Julie ; Saucier, Jill ; Reichard, Mason V. ; Meinkoth, Katrina ; Meinkoth, James H. ; Andrews, Blaine ; Ullom, Steve ; Ewing, Sidney A. ; Chandrashekar, Ramaswamy</creator><creatorcontrib>Little, Susan E. ; O’Connor, Thomas P. ; Hempstead, Julie ; Saucier, Jill ; Reichard, Mason V. ; Meinkoth, Katrina ; Meinkoth, James H. ; Andrews, Blaine ; Ullom, Steve ; Ewing, Sidney A. ; Chandrashekar, Ramaswamy</creatorcontrib><description>We used PCR and a novel serologic assay to determine infection and exposure rates to
Ehrlichia ewingii in dogs from an area of northeast Oklahoma and northwest Arkansas where
Amblyomma americanum ticks are abundant. Of 143 dogs assayed, 13 (9.1%) harbored
E. ewingii by PCR and 64 (44.8%) had antibodies to
E. ewingii detected using a peptide-based microtiter plate ELISA. Dogs were more likely (
P
=
0.001) to be positive by PCR if sampled in August (30.8%) but no association was found between seropositive status and month of collection of sample (
P
>
0.05). Additional testing revealed PCR evidence of
Ehrlichia chaffeensis (4/143; 2.8%) and
Anaplasma platys (5/143; 3.5%) as well as antibodies reactive to
E. chaffeensis (25/143; 17.5%),
Ehrlichia canis (2/143; 1.4%), and
Anaplasma spp. (8/143; 5.6%). Testing of another 200 dogs from the area revealed additional PCR and/or serologic evidence of
E. ewingii,
E. canis,
E. chaffeensis, and
A. platys. None of the 343 dogs evaluated had evidence of
Borrelia burgdorferi exposure. These data support the interpretation that
E. ewingii may be the primary agent of canine ehrlichiosis in this region, and suggest that diagnostic evaluation of dogs suspected to have a tick-borne disease should include assays targeting this organism.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2010.05.006</identifier><identifier>PMID: 20541322</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amblyomma americanum ; Anaplasma phagocytophilum ; Anaplasma platys ; Anaplasmosis ; Animals ; Antibodies, Bacterial - blood ; antibody detection ; Arkansas - epidemiology ; Borrelia burgdorferi ; Canine ; disease prevalence ; disease surveillance ; dog diseases ; Dog Diseases - epidemiology ; Dogs ; Ehrlichia - physiology ; Ehrlichia canis ; Ehrlichia chaffeensis ; Ehrlichia ewingii ; Ehrlichiosis ; Ehrlichiosis - epidemiology ; Ehrlichiosis - veterinary ; epidemiological studies ; microscopy ; molecular epidemiology ; Oklahoma - epidemiology ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; Prevalence ; risk assessment ; screening ; serodiagnosis ; seroprevalence ; Tick-Borne Diseases - epidemiology ; Tick-Borne Diseases - veterinary</subject><ispartof>Veterinary parasitology, 2010-09, Vol.172 (3), p.355-360</ispartof><rights>2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-c71721ec74adcef41c234eb30caac3a55e243272a423f5e10b279a6f94ec9d503</citedby><cites>FETCH-LOGICAL-c385t-c71721ec74adcef41c234eb30caac3a55e243272a423f5e10b279a6f94ec9d503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2010.05.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20541322$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Little, Susan E.</creatorcontrib><creatorcontrib>O’Connor, Thomas P.</creatorcontrib><creatorcontrib>Hempstead, Julie</creatorcontrib><creatorcontrib>Saucier, Jill</creatorcontrib><creatorcontrib>Reichard, Mason V.</creatorcontrib><creatorcontrib>Meinkoth, Katrina</creatorcontrib><creatorcontrib>Meinkoth, James H.</creatorcontrib><creatorcontrib>Andrews, Blaine</creatorcontrib><creatorcontrib>Ullom, Steve</creatorcontrib><creatorcontrib>Ewing, Sidney A.</creatorcontrib><creatorcontrib>Chandrashekar, Ramaswamy</creatorcontrib><title>Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>We used PCR and a novel serologic assay to determine infection and exposure rates to
Ehrlichia ewingii in dogs from an area of northeast Oklahoma and northwest Arkansas where
Amblyomma americanum ticks are abundant. Of 143 dogs assayed, 13 (9.1%) harbored
E. ewingii by PCR and 64 (44.8%) had antibodies to
E. ewingii detected using a peptide-based microtiter plate ELISA. Dogs were more likely (
P
=
0.001) to be positive by PCR if sampled in August (30.8%) but no association was found between seropositive status and month of collection of sample (
P
>
0.05). Additional testing revealed PCR evidence of
Ehrlichia chaffeensis (4/143; 2.8%) and
Anaplasma platys (5/143; 3.5%) as well as antibodies reactive to
E. chaffeensis (25/143; 17.5%),
Ehrlichia canis (2/143; 1.4%), and
Anaplasma spp. (8/143; 5.6%). Testing of another 200 dogs from the area revealed additional PCR and/or serologic evidence of
E. ewingii,
E. canis,
E. chaffeensis, and
A. platys. None of the 343 dogs evaluated had evidence of
Borrelia burgdorferi exposure. These data support the interpretation that
E. ewingii may be the primary agent of canine ehrlichiosis in this region, and suggest that diagnostic evaluation of dogs suspected to have a tick-borne disease should include assays targeting this organism.</description><subject>Amblyomma americanum</subject><subject>Anaplasma phagocytophilum</subject><subject>Anaplasma platys</subject><subject>Anaplasmosis</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>antibody detection</subject><subject>Arkansas - epidemiology</subject><subject>Borrelia burgdorferi</subject><subject>Canine</subject><subject>disease prevalence</subject><subject>disease surveillance</subject><subject>dog diseases</subject><subject>Dog Diseases - epidemiology</subject><subject>Dogs</subject><subject>Ehrlichia - physiology</subject><subject>Ehrlichia canis</subject><subject>Ehrlichia chaffeensis</subject><subject>Ehrlichia ewingii</subject><subject>Ehrlichiosis</subject><subject>Ehrlichiosis - epidemiology</subject><subject>Ehrlichiosis - veterinary</subject><subject>epidemiological studies</subject><subject>microscopy</subject><subject>molecular epidemiology</subject><subject>Oklahoma - epidemiology</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Prevalence</subject><subject>risk assessment</subject><subject>screening</subject><subject>serodiagnosis</subject><subject>seroprevalence</subject><subject>Tick-Borne Diseases - epidemiology</subject><subject>Tick-Borne Diseases - veterinary</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vEzEQhq0K1KaBf4DAN06bjr_WuxckVJWCVIlDm0svluOdTRxt1sH2lvbf42gLR04jzTwz8-oh5AODFQNWX-1XT5iPNq44lBaoFUB9Rhas0aLiSsEbsgABspLA9AW5TGkPABJqfU4uOCjJBOcL8nizi4N3O28p_vbj1nvqxx5d9mGkduwoPh9DmiLSaDOmMqRd2Cbax3CgeYc0hSnvHI452oGuR5-xo_f5xL4jb3s7JHz_Wpdk_e3m4fp7dffz9sf117vKiUblymmmOUOnpe0c9pI5LiRuBDhrnbBKIZeCa24lF71CBhuuW1v3rUTXdgrEknye7x5j-DVhyubgk8NhsCOGKRmtRNvWrawLKWfSxZBSxN4coz_Y-GIYmJNUszezVHOSakCZIrWsfXx9MG0O2P1b-muxAJ9moLfB2G30yazvywUBrGnqpgRYki8zgUXEk8dokvM4Oux8LLJNF_z_M_wBPdKUbg</recordid><startdate>20100920</startdate><enddate>20100920</enddate><creator>Little, Susan E.</creator><creator>O’Connor, Thomas P.</creator><creator>Hempstead, Julie</creator><creator>Saucier, Jill</creator><creator>Reichard, Mason V.</creator><creator>Meinkoth, Katrina</creator><creator>Meinkoth, James H.</creator><creator>Andrews, Blaine</creator><creator>Ullom, Steve</creator><creator>Ewing, Sidney A.</creator><creator>Chandrashekar, Ramaswamy</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100920</creationdate><title>Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States</title><author>Little, Susan E. ; O’Connor, Thomas P. ; Hempstead, Julie ; Saucier, Jill ; Reichard, Mason V. ; Meinkoth, Katrina ; Meinkoth, James H. ; Andrews, Blaine ; Ullom, Steve ; Ewing, Sidney A. ; Chandrashekar, Ramaswamy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-c71721ec74adcef41c234eb30caac3a55e243272a423f5e10b279a6f94ec9d503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amblyomma americanum</topic><topic>Anaplasma phagocytophilum</topic><topic>Anaplasma platys</topic><topic>Anaplasmosis</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>antibody detection</topic><topic>Arkansas - epidemiology</topic><topic>Borrelia burgdorferi</topic><topic>Canine</topic><topic>disease prevalence</topic><topic>disease surveillance</topic><topic>dog diseases</topic><topic>Dog Diseases - epidemiology</topic><topic>Dogs</topic><topic>Ehrlichia - physiology</topic><topic>Ehrlichia canis</topic><topic>Ehrlichia chaffeensis</topic><topic>Ehrlichia ewingii</topic><topic>Ehrlichiosis</topic><topic>Ehrlichiosis - epidemiology</topic><topic>Ehrlichiosis - veterinary</topic><topic>epidemiological studies</topic><topic>microscopy</topic><topic>molecular epidemiology</topic><topic>Oklahoma - epidemiology</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Prevalence</topic><topic>risk assessment</topic><topic>screening</topic><topic>serodiagnosis</topic><topic>seroprevalence</topic><topic>Tick-Borne Diseases - epidemiology</topic><topic>Tick-Borne Diseases - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Little, Susan E.</creatorcontrib><creatorcontrib>O’Connor, Thomas P.</creatorcontrib><creatorcontrib>Hempstead, Julie</creatorcontrib><creatorcontrib>Saucier, Jill</creatorcontrib><creatorcontrib>Reichard, Mason V.</creatorcontrib><creatorcontrib>Meinkoth, Katrina</creatorcontrib><creatorcontrib>Meinkoth, James H.</creatorcontrib><creatorcontrib>Andrews, Blaine</creatorcontrib><creatorcontrib>Ullom, Steve</creatorcontrib><creatorcontrib>Ewing, Sidney A.</creatorcontrib><creatorcontrib>Chandrashekar, Ramaswamy</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Little, Susan E.</au><au>O’Connor, Thomas P.</au><au>Hempstead, Julie</au><au>Saucier, Jill</au><au>Reichard, Mason V.</au><au>Meinkoth, Katrina</au><au>Meinkoth, James H.</au><au>Andrews, Blaine</au><au>Ullom, Steve</au><au>Ewing, Sidney A.</au><au>Chandrashekar, Ramaswamy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2010-09-20</date><risdate>2010</risdate><volume>172</volume><issue>3</issue><spage>355</spage><epage>360</epage><pages>355-360</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>We used PCR and a novel serologic assay to determine infection and exposure rates to
Ehrlichia ewingii in dogs from an area of northeast Oklahoma and northwest Arkansas where
Amblyomma americanum ticks are abundant. Of 143 dogs assayed, 13 (9.1%) harbored
E. ewingii by PCR and 64 (44.8%) had antibodies to
E. ewingii detected using a peptide-based microtiter plate ELISA. Dogs were more likely (
P
=
0.001) to be positive by PCR if sampled in August (30.8%) but no association was found between seropositive status and month of collection of sample (
P
>
0.05). Additional testing revealed PCR evidence of
Ehrlichia chaffeensis (4/143; 2.8%) and
Anaplasma platys (5/143; 3.5%) as well as antibodies reactive to
E. chaffeensis (25/143; 17.5%),
Ehrlichia canis (2/143; 1.4%), and
Anaplasma spp. (8/143; 5.6%). Testing of another 200 dogs from the area revealed additional PCR and/or serologic evidence of
E. ewingii,
E. canis,
E. chaffeensis, and
A. platys. None of the 343 dogs evaluated had evidence of
Borrelia burgdorferi exposure. These data support the interpretation that
E. ewingii may be the primary agent of canine ehrlichiosis in this region, and suggest that diagnostic evaluation of dogs suspected to have a tick-borne disease should include assays targeting this organism.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20541322</pmid><doi>10.1016/j.vetpar.2010.05.006</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary parasitology, 2010-09, Vol.172 (3), p.355-360 |
| issn | 0304-4017 1873-2550 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amblyomma americanum Anaplasma phagocytophilum Anaplasma platys Anaplasmosis Animals Antibodies, Bacterial - blood antibody detection Arkansas - epidemiology Borrelia burgdorferi Canine disease prevalence disease surveillance dog diseases Dog Diseases - epidemiology Dogs Ehrlichia - physiology Ehrlichia canis Ehrlichia chaffeensis Ehrlichia ewingii Ehrlichiosis Ehrlichiosis - epidemiology Ehrlichiosis - veterinary epidemiological studies microscopy molecular epidemiology Oklahoma - epidemiology polymerase chain reaction Polymerase Chain Reaction - veterinary Prevalence risk assessment screening serodiagnosis seroprevalence Tick-Borne Diseases - epidemiology Tick-Borne Diseases - veterinary |
| title | Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United States |
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