Isoelectric focusing and isoelectric points of bovine liver histones
A technique for isoelectric focusing of total histones in very narrow pH gradients is described. The isoelectric focusing was performed in 5% acrylamide gels at the pH range 9–11 in long quartz tubes (24 cm) in a nitrogen atmosphere. The total bovine liver histones separated into five main fractions...
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| Veröffentlicht in: | Analytical biochemistry 1980-05, Vol.104 (2), p.499-505 |
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| creator | Valkonen, Kaija H. Piha, R.S. |
| description | A technique for isoelectric focusing of total histones in very narrow pH gradients is described. The isoelectric focusing was performed in 5% acrylamide gels at the pH range 9–11 in long quartz tubes (24 cm) in a nitrogen atmosphere. The total bovine liver histones separated into five main fractions which were identified as H1, H3, H2B, H2A, and H4 histones, and their apparent isoelectric points were determined. The main fractions were further divided into several subfractions, the maximal number of bands being 12. The isoelectric point for H1 histone in 6.25
m urea solution in the presence of a nitrogen atmosphere was 8.90, and the corresponding values for H3, H2B, H2A, and H4 histones were 9.80, 9.90, 10.10, and 10.25, respectively. The focusing technique described here has a high resolution, reproducibility, and sensitivity. The technique can be used for preparative and quantitative analysis and for studies on specificity and developmental changes of histones. |
| doi_str_mv | 10.1016/0003-2697(80)90107-4 |
| format | Article |
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m urea solution in the presence of a nitrogen atmosphere was 8.90, and the corresponding values for H3, H2B, H2A, and H4 histones were 9.80, 9.90, 10.10, and 10.25, respectively. The focusing technique described here has a high resolution, reproducibility, and sensitivity. The technique can be used for preparative and quantitative analysis and for studies on specificity and developmental changes of histones.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(80)90107-4</identifier><identifier>PMID: 7446976</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Cell Nucleus - analysis ; Electrophoresis, Polyacrylamide Gel ; Histones - isolation & purification ; Hydrogen-Ion Concentration ; Isoelectric Focusing ; Isoelectric Point ; Liver - analysis</subject><ispartof>Analytical biochemistry, 1980-05, Vol.104 (2), p.499-505</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-69069b1b4e47ffddf4a6ca76b1d8a04da563f1858305290ba3393b7bbf5d3feb3</citedby><cites>FETCH-LOGICAL-c357t-69069b1b4e47ffddf4a6ca76b1d8a04da563f1858305290ba3393b7bbf5d3feb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269780901074$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7446976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valkonen, Kaija H.</creatorcontrib><creatorcontrib>Piha, R.S.</creatorcontrib><title>Isoelectric focusing and isoelectric points of bovine liver histones</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A technique for isoelectric focusing of total histones in very narrow pH gradients is described. The isoelectric focusing was performed in 5% acrylamide gels at the pH range 9–11 in long quartz tubes (24 cm) in a nitrogen atmosphere. The total bovine liver histones separated into five main fractions which were identified as H1, H3, H2B, H2A, and H4 histones, and their apparent isoelectric points were determined. The main fractions were further divided into several subfractions, the maximal number of bands being 12. The isoelectric point for H1 histone in 6.25
m urea solution in the presence of a nitrogen atmosphere was 8.90, and the corresponding values for H3, H2B, H2A, and H4 histones were 9.80, 9.90, 10.10, and 10.25, respectively. The focusing technique described here has a high resolution, reproducibility, and sensitivity. The technique can be used for preparative and quantitative analysis and for studies on specificity and developmental changes of histones.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cell Nucleus - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Histones - isolation & purification</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isoelectric Focusing</subject><subject>Isoelectric Point</subject><subject>Liver - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEQx4MotVa_gcKeRA-rkyab3b0IUl-Fghc9hzwmGtluarJb8Nu7tUU8eRqY_2OYHyGnFK4oUHENACyfirq8qOCyBgplzvfImEItcmBQ75Pxr-WQHKX0AUApL8SIjErOh60Yk7t5Ctig6aI3mQumT759y1RrM_9HWAXfdikLLtNh7VvMGr_GmL371IUW0zE5cKpJeLKbE_L6cP8ye8oXz4_z2e0iN6wou1zUIGpNNUdeOmet40oYVQpNbaWAW1UI5mhVVAyKaQ1aMVYzXWrtCsscajYh59veVQyfPaZOLn0y2DSqxdAnWRasElMKg5FvjSaGlCI6uYp-qeKXpCA38OSGjNyQkRXIH3iSD7GzXX-vl2h_Qztag36z1XF4cu0xymQ8tgatjwMpaYP__8A3K8x-rw</recordid><startdate>19800515</startdate><enddate>19800515</enddate><creator>Valkonen, Kaija H.</creator><creator>Piha, R.S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800515</creationdate><title>Isoelectric focusing and isoelectric points of bovine liver histones</title><author>Valkonen, Kaija H. ; Piha, R.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-69069b1b4e47ffddf4a6ca76b1d8a04da563f1858305290ba3393b7bbf5d3feb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cell Nucleus - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Histones - isolation & purification</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isoelectric Focusing</topic><topic>Isoelectric Point</topic><topic>Liver - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valkonen, Kaija H.</creatorcontrib><creatorcontrib>Piha, R.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valkonen, Kaija H.</au><au>Piha, R.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isoelectric focusing and isoelectric points of bovine liver histones</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1980-05-15</date><risdate>1980</risdate><volume>104</volume><issue>2</issue><spage>499</spage><epage>505</epage><pages>499-505</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A technique for isoelectric focusing of total histones in very narrow pH gradients is described. The isoelectric focusing was performed in 5% acrylamide gels at the pH range 9–11 in long quartz tubes (24 cm) in a nitrogen atmosphere. The total bovine liver histones separated into five main fractions which were identified as H1, H3, H2B, H2A, and H4 histones, and their apparent isoelectric points were determined. The main fractions were further divided into several subfractions, the maximal number of bands being 12. The isoelectric point for H1 histone in 6.25
m urea solution in the presence of a nitrogen atmosphere was 8.90, and the corresponding values for H3, H2B, H2A, and H4 histones were 9.80, 9.90, 10.10, and 10.25, respectively. The focusing technique described here has a high resolution, reproducibility, and sensitivity. The technique can be used for preparative and quantitative analysis and for studies on specificity and developmental changes of histones.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7446976</pmid><doi>10.1016/0003-2697(80)90107-4</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Cattle Cell Nucleus - analysis Electrophoresis, Polyacrylamide Gel Histones - isolation & purification Hydrogen-Ion Concentration Isoelectric Focusing Isoelectric Point Liver - analysis |
| title | Isoelectric focusing and isoelectric points of bovine liver histones |
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